Antiviral potential of green synthesized silver nanoparticles of lampranthus coccineus and malephora lutea
Date
2019
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Dove Medical Press Ltd.
Series Info
International Journal of Nanomedicine
14
14
Scientific Journal Rankings
Abstract
Background: Viral and microbial infections constitute one of the most important life-threatening problems. The emergence of new viral and bacterial infectious diseases increases the demand for new therapeutic drugs. Purpose: The objective of this study was to use the aqueous and hexane extracts of Lampranthus coccineus and Malephora lutea F. Aizoaceae for the synthesis of silver nanoparticles, and to investigate its possible antiviral activity. In addition to the investigation of the phytochemical composition of the crude methanolic extracts of the two plants through UPLC-MS metabolomic profiling, and it was followed by molecular docking in order to explore the chemical compounds that might contribute to the antiviral potential. Methods: The formation of SNPs was further confirmed using a transmission electron microscope (TEM), UV-Visible spectroscopy and Fourier transform infrared spectroscopy. The antiviral activity of the synthesized nanoparticles was evaluated using MTT assay against HSV-1, HAV-10 virus and Coxsackie B4 virus. Metabolomics profiling was performed using UPLC-MS and molecular docking was performed via Autodock4 and visualization was done using the Discovery studio. Results: The early signs of SNPs synthesis were detected by a color change from yellow to reddish brown color. The TEM analysis of SNPs showed spherical nanoparticles with mean size ranges between 10.12 nm to 27.89 nm, and 8.91 nm 14.48 nm for Lampranthus coccineus and Malephora lutea aqueous and hexane extracts respectively. The UV-Visible spectrophotometric analysis showed an absorption peak at ?max of 417 nm.The green synthesized SNPs of L. coccineus and M. lutea showed remarkable antiviral activity against HSV-1, HAV-10, and CoxB4 virus. Metabolomics profiling of the methanolic extract of L. coccineus and M. lutea resulted in identifying 12 compounds. The docking study predicted the patterns of interactions between the compounds of L. coccineus and M. lutea with herpes simplex thymidine kinase, hepatitis A 3c proteinase, and Coxsackievirus B4 3c protease, which was similar to those of the co-crystal inhibitors and this can provide a supposed explanation for the antiviral activity of the aqueous and nano extracts of L. coccineus and M. lutea. Conclusion: These results highlight that SNPs of L. coccineus and M. lutea could have antiviral activity against HSV-1, HAV-10, and CoxB4 virus. � 2019 Haggag et al. This work is published and licensed by Dove Medical Press Limited.
Description
Scopus
Keywords
Antiviral, Lampranthus coccineus, Malephora lutea, Metabolomics profiling, Silver nanoparticles, hexane, Lampranthus coccineus extract, Malephora lutea extract, plant extract, proteinase, silver nanoparticle, thymidine kinase, unclassified drug, virus 3c proteinase, antivirus agent, ligand, metal nanoparticle, plant extract, silver, aerial plant part, Aizoaceae, animal cell, antiviral activity, aqueous solution, Article, controlled study, Coxsackievirus B4, crystal structure, drug structure, drug synthesis, Fourier transform infrared spectroscopy, green chemistry, Hepatitis A virus, Hepatitis A virus 10, Human alphaherpesvirus 1, IC50, Lampranthus coccineus, liquid chromatography-mass spectrometry, Malephora lutea, metabolomics, molecular docking, MTT assay, nonhuman, particle size, phytochemistry, solvent extraction, transmission electron microscopy, ultraviolet visible spectroscopy, Vero cell line, virus cell interaction, animal, cell death, chemistry, Chlorocebus aethiops, drug effect, infrared spectroscopy, ultrastructure, ultraviolet spectrophotometry, Aizoaceae, Animals, Antiviral Agents, Cell Death, Cercopithecus aethiops, Green Chemistry Technology, Ligands, Metabolomics, Metal Nanoparticles, Molecular Docking Simulation, Plant Extracts, Silver, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, Vero Cells