MSA Repository "MSAR"
MSAR University's Digital Repository is a documentation and digitization of all university outcomes that are of effective value in the scientific and academic community and reflects the university's image, work, and effective contribution to society Through MSAR Digital Repository, the university managed to collect, store, archive and publish digital content - including documents, audio files, images and data sets - all in a safe place. MSAR is one of the strongest University Digital Repositories in Egypt and documented in the DSPACE community with its latest versions.
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Recent Submissions
β-Galactosidase inhibition explored by biochemical methods and in silico 1 studies for plant polyphenols
(Academic Press Inc., 2025-07-25) Ahmed Zayed; Karima Sayah; Kalicharan Sharma; Rasha Ali Radwan; Shahira M. Ezzat
β-Galactosidase is a lysosomal enzyme whose deficiency is associated with genetic disorders such as GM1 gangliosidosis, prompting the search for novel enzyme modulators with therapeutic potential. The current study evaluated the inhibitory potential of selected natural polyphenols against β-galactosidase using a combined approach of biochemical assays and computational modeling. Sixteen plant-derived compounds were initially screened through molecular docking against Aspergillus oryzae β-galactosidase. Among these, hesperidin, rutin, and chlorogenic acid exhibited the most favorable interactions and were subsequently assessed through in vitro enzyme inhibition assays and MM/GBSA binding energy calculations. These compounds showed potential inhibitory effects and stable binding within the enzyme’s active site. Although classical pharmacological chaperone activity was not directly demonstrated, the observed modulation of enzyme function suggests potential for further development of these polyphenols as structurally distinct glycosidase inhibitors. The findings provide a basis for future investigations aimed at natural product-based strategies to manage lysosomal storage disorders such as GM1 gangliosidosis.
Pulp response following direct pulp capping with Tideglusib and mineral trioxide aggregate: an animal study
(BioMed Central Ltd, 2025-07-19) Momen M. Mhmod; Ehab E. Hassanien; Ashraf M. Abu-Seida; Salma H. EL Ashry; Mohamed M. Nagy; Sara H. Fahmy; Elhassan E.E. Hassanein
Background Several biomaterials have been employed for direct pulp capping (DPC) with varying degrees of
success. This study evaluated the pulp response following DPC with a new material developed from glycogen
synthase kinase-3 inhibitors (Tideglusib) and mineral trioxide aggregate (MTA).
Methods Class V cavities with pulp exposure were conducted on 56 teeth in two adult male mongrel dogs. Based
on the evaluation periods, these teeth were divided into two major groups at random (28 teeth/dog each). Groups
A and B underwent histopathology evaluations three and eight weeks following DPC, respectively. Depending on
the capping material used, each main group was further divided into two equal subgroups (14 teeth each). ProRoot
white MTA was applied directly to the exposed vital pulps in subgroup 1. While subgroup 2’s exposed pulps were
immediately capped with resorbable collagen that had been soaked in a freshly made 50 nM Tideglusib drug
solution. Glass ionomer filling was then used to seal the access cavities. Every specimen underwent histological
evaluation and was scored according to the number of inflammatory cells, the disorganization of the pulp tissue, and
the formation of calcific bridges. All data were statistically examined.
Results In both groups A and B, subgroup 2 showed a statistically significant increase in the number of inflammatory
cells and pulp tissue disorganization compared to subgroup 1 (P<0.05). In both groups A and B, there was no
statistically significant difference in the formation of new hard tissue between subgroups 1 and 2 (P=0.157).
Conclusion When used as direct vital pulp capping materials in a dog model, Tideglusib causes more soft tissue
disorganization and an inflammatory response inside the pulp cavity than ProRoot white MTA.
Filamentous prophages in the genomes of Acinetobacter baumannii from egypt: impact on biofilm formation and the potential to induce enterotoxicity
(BioMed Central Ltd, 2025-07-23) Samira M. Hamed; Amira Abdel-Daim; Samer A. Tadros; Mai M. Zafer
Filamentous phages (FPs) have been recently isolated from Acinetobacter baumannii. While FPs are known to
modulate the virulence of some Gram-negative pathogens, their role in A. baumannii has not been fully explored.
This study analyzed 18 clinical isolates of A. baumannii from global clones (GC), with draft genomes generated
by Illumina sequencing. All isolates were screened for filamentous prophage (FPP) genomes using the Zonula
occludens toxin (Zot)-coding gene as a marker. Nine out of the 18 isolates were found to carry zot genes. The
complete sequences of four FPPs were predicted. FPPs were exclusively found within GC1, GC7, and GC9 strains.
Among the A. baumannii genomes deposited in the NCBI genomic database, FPPs were found to be disseminated
in 42 Pasteur STs spanning at least six GCs, most commonly GC1. The impact of FPs on biofilm formation in A.
baumannii was investigated using crystal violet assay. None of the zot-negative isolates formed strong biofilms,
while six (66.6%) zot-positive isolates did. The biofilm indices of zot-positive isolates were significantly higher
compared to zot-negative isolates. The potential enterotoxicity of the zot-positive strains was also assessed using in
silico and experimental methods. The cytotoxic effect of cell-free supernatants (CFSs) on Caco-2 cells was measured
by the MTT assay. Cells treated with CFSs from zot-positive strains exhibited significantly higher cytotoxicity than
those treated with CFSs from zot-negative strains. Upon injecting the CFS of a zot-positive strain intraperitoneally
into BALB/c mice, severe diarrhea was observed within 6 h. Histological examination of the intestinal tissue 24 h
post-injection revealed significant changes. In conclusion, this study suggests that FPPs are widely disseminated
in A. baumannii GCs and may enhance biofilm formation and enterotoxicity, potentially contributing to the
pathogen’s virulence.
Vitamin D Levels, Periodontal Parameters, and VDR Gene Polymorphism in Dental Implant Osseointegration Outcomes: A Case-Control Study in Egyptians
(Bentham Science Publishers, 2025-07-20) Al-Hassan Diab; Mihad Ibrahim; Amal Ahmed Mohamed; Omnia Esmail; Mahmoud Mohammed Metwally; Ahmed Raslan Mahmoud Alwan; Mohammad Abd Elhameed Ahmed Alwaseef; Ahmed Abdelhameed Abozeed; Rehab Elhusseini Mohamed Elwakeel; Abbas Abbas; Heba H. El-Osaily; Ayat G. Ali; Hanan Hegazy; Rasha Salama; Sherief AbdElsalam; Karima Nasraldin
Introduction: Failure of dental implants due to inadequate osseointegration continues to pose a major clinical issue, with increasing evidence suggesting that systemic and genetic factors play a role in the outcomes. It is proposed that vitamin D deficiency and genetic variations, such as those in the vitamin D receptor (VDR) gene, may affect bone metabolism and the success of implants. This case-control study aimed to evaluate the association of serum vitamin D levels and VDR gene polymorphism (SNP rs228570) with dental implant osseointegration success or failure. Methods: A case-control study with 42 cases of implant failure and 42 controls with successful osseointegration was conducted in patients aged 31–60 years. Sociodemographic, clinical, and periodontal parameters were analyzed, and VDR SNP rs228570 was genotyped using real-time PCR, and serum vitamin D, TNF, and IL-6 levels were measured. Statistical analysis was performed using SPSS v28.0, with univariate and multivariable logistic regression with significance (p < 0.05). Results: No significant association was found between VDR gene polymorphism (SNP rs228570) and implant failure (p > 0.05). However, there is a significant association between higher vitamin D levels and successful osseointegration. Vitamin D levels were significantly higher in the successful group (36.85 ng/ml ± 11.55) compared to the failed group (17.03 ng/ml ± 9.16) (p < 0.001). Clinical parameters revealed significant differences, with the successful group showing lower bleeding on probing (BOP) at 25.15% (SD ± 5.31) compared to 42.46% (SD ± 7.59) in the failed group (p < 0.001) and a shallower probing depth of 2.21 mm (SD ± 0.85) compared to 5.62 mm (SD ± 1.32) in the failed group (p < 0.001). Biochemical markers such as TNF and IL-6 did not show significant differences between the groups, P-value = 0.181 and 0.186, respectively. Discussion: The study highlights the importance of vitamin D levels and clinical parameters such as BOP and PD in predicting osseointegration outcomes. Conclusion: The study highlights the importance of vitamin D levels and clinical parameters such as BOP and PD in predicting osseointegration outcomes. Although VDR gene polymorphism showed no significant association with implant failure, higher vitamin D levels were positively correlated with successful osseointegration. These findings suggest that optimizing vitamin D levels and managing peri-implant health may improve dental implant success rates, emphasizing clinical over genetic predictors. © 2025 The Author(s). Published by Bentham Open
Gamma-irradiated chitosan-HPMC/Ulva lactuca based hydrogel for heavy metal remediation from aqueous solutions
(Publisher Elsevier B.V., 2025-07-18) Asmaa Sayed; Thomas Emad; Sara Badawi; Ghada A. Mahmoud; Manar El-Sayed Abdel-Raouf
This study focuses on the novel bio-composite hydrogel made from chitosan (CS), hydroxypropyl methylcellulose (HPMC), polyacrylamide (PAAm), and green algae Ulva lactuca (Ul), which is synthesized to effectively remove Cu(II) and Co(II) ions from simulated aqueous solutions using gamma irradiation. The hydrogels, whether or not they had Ulva lactuca, were labeled CS/HPMC/PAAm and CS/HPMC/PAAm/Ul. The process was tested by changing the acrylamide concentration, the dose of gamma irradiation, and adding Ulva. Analyzing the Ulva with BET and EDX helped prove that its addition to the hydrogel increases surface area, porosity, tolerance to heat, and roughness of the surface. In both metal systems, the hydrogel strongly favored Co(II) over Fe(II). Kinetic analysis favored the pseudo-second-order (PSO) model, indicating chemisorption as the rate-controlling step. The Jovanovic and Redlich–Peterson isotherm models provided the best fit to the experimental data. The results suggested that the adsorption process involves a combination of physical and chemical interactions. Reusability tests over five cycles confirmed the hydrogel's durability and regeneration potential. The CS/HPMC/PAAm/Ul hydrogel is an eco-friendly and efficient sorbent, making it a strong candidate for heavy metal remediation in environmental applications. © 2025 Elsevier B.V.