DEVELOPMENT AND VALIDATION OF CHROMATOGRAPHIC AND SPECTROSCOPIC METHODS FOR ESTIMATION OF REPAGLINIDE AND METFORMIN HCl IN COMBINED DOSAGE FORM
| dc.Affiliation | October University for modern sciences and Arts (MSA) | |
| dc.contributor.author | M. Fouad, Manal | |
| dc.contributor.author | S. Rashed, Noha | |
| dc.date.accessioned | 2019-10-19T08:47:47Z | |
| dc.date.available | 2019-10-19T08:47:47Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Two simple, accurate and reproducible methods have been developed for the determination of repaglinide and metformin as bulk or simultaneously in their combined dosage form. The first is a reversed phase HPLC method using C18 column, mobile phase consisting of methanol-0.2% heptane sulphonate sodium (70:30 v/v) at a flow rate of 1ml/min and UV detection at 240 nm. Beer's plot shows good correlations in the concentration range of 1-10 μgml-1 for both drugs. The second method is a derivative spectrophotometry, in which the two drugs were quantified using second derivative responses at 287 nm for repaglinide and at 234 or 252 nm for metformin with a linearity range of 5- 40 or 2-12 μgml-1 for the two drugs, respectively. The proposed methods were validated and successfully applied to the determination of both drugs in their pharmaceutical formulation with mean recoveries of 99.58-100.45 ± 0.386-0.931. The results obtained were statistically compared to those obtained from a reference method and were found to be in good agreement. | en_US |
| dc.description.uri | https://www.scimagojr.com/journalsearch.php?q=21100853545&tip=sid&clean=0 | |
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| dc.identifier.doi | https://doi.org/ | |
| dc.identifier.issn | 2230-7346 | |
| dc.identifier.other | https://doi.org/ | |
| dc.identifier.uri | http://central-library.msa.edu.eg:8009/xmlui/handle/123456789/396 | |
| dc.language.iso | en | en_US |
| dc.publisher | Journal of Global Trends in Pharmaceutical Sciences | en_US |
| dc.relation.ispartofseries | JGTPS,;Vol. 5(3): 1844- 1848 | |
| dc.subject | Repaglinide, metformin, | en_US |
| dc.subject | HPLC, | en_US |
| dc.subject | second-derivative | en_US |
| dc.subject | spectrophotometry, | en_US |
| dc.subject | pharmaceutical formulation. | en_US |
| dc.title | DEVELOPMENT AND VALIDATION OF CHROMATOGRAPHIC AND SPECTROSCOPIC METHODS FOR ESTIMATION OF REPAGLINIDE AND METFORMIN HCl IN COMBINED DOSAGE FORM | en_US |
| dc.type | Article | en_US |