DEVELOPMENT AND VALIDATION OF CHROMATOGRAPHIC AND SPECTROSCOPIC METHODS FOR ESTIMATION OF REPAGLINIDE AND METFORMIN HCl IN COMBINED DOSAGE FORM
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Date
2014
Authors
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Journal of Global Trends in Pharmaceutical Sciences
Series Info
JGTPS,;Vol. 5(3): 1844- 1848
Doi
Scientific Journal Rankings
Abstract
Two simple, accurate and reproducible methods have been developed for the
determination of repaglinide and metformin as bulk or simultaneously in their combined
dosage form. The first is a reversed phase HPLC method using C18 column, mobile
phase consisting of methanol-0.2% heptane sulphonate sodium (70:30 v/v) at a flow rate
of 1ml/min and UV detection at 240 nm. Beer's plot shows good correlations in the
concentration range of 1-10 μgml-1 for both drugs. The second method is a derivative
spectrophotometry, in which the two drugs were quantified using second derivative
responses at 287 nm for repaglinide and at 234 or 252 nm for metformin with a linearity
range of 5- 40 or 2-12 μgml-1 for the two drugs, respectively. The proposed methods were
validated and successfully applied to the determination of both drugs in their
pharmaceutical formulation with mean recoveries of 99.58-100.45 ± 0.386-0.931. The
results obtained were statistically compared to those obtained from a reference method
and were found to be in good agreement.
Description
Keywords
Repaglinide, metformin,, HPLC,, second-derivative, spectrophotometry,, pharmaceutical formulation.
Citation
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