Characterization of a Novel α-L-fucosidase from Truepera sp. for efficient transfucosylation and 2’-fucosyllactose biosynthesis
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American Chemical Society
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Journal of Agricultural and Food Chemistry ; Volume 73 , Issue 39 , Pages 24830 - 24844
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Abstract
Fucose is a key deoxyhexose found in polysaccharides, glycolipids, and glycoproteins. 2′-Fucosyllactose (2′FL), a major human milk oligosaccharide with health benefits for infants, faces production challenges due to the limited availability of efficient α-l-fucosidases. Here, we present a novel α-l-fucosidase, True-Fuc, from Truepera sp. heterologously expressed for 2′FL biosynthesis. True-Fuc, a GH29A family enzyme (50 kDa), showed optimal activity at 50 °C and pH 8.0, hydrolyzing para-nitrophenyl-α-l-fucopyranoside (pNP-α-Fuc), Lewisa, and Lewisxsubstrates. It catalyzed 2′FL synthesis via transfucosylation using pNP-α-Fuc and lactose with minimal degradation of 2′FL and 3-fucosyllactose (3FL). Molecular dynamics simulations revealed that loops 1–4 surrounding the substrate pocket mediate substrate recognition, while the flexible C-terminal loop 5 plays a minor role. These results establish True-Fuc as a promising tool for cost-effective 2′FL production and novel glycoside synthesis. © 2025 American Chemical Society
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SJR 2024
1.215
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H-Index
358
Citation
Hamouda, H. I., El-Sayed, M. H., Nassar, H. N., El-Gendy, N. S., Meawad, S. B., Wang, W., Li, T., & Yin, H. (2025). Characterization of a Novel α-l-Fucosidase from Truepera sp. for Efficient Transfucosylation and 2′-Fucosyllactose Biosynthesis. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/acs.jafc.5c06107