Liquid chromatography–tandem MS/MS method for simultaneous quantification of paracetamol, chlorzoxazone and aceclofenac in human plasma: An application to a clinical pharmacokinetic study

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorMohamed D.
dc.contributor.authorHegazy M.A.
dc.contributor.authorElshahed M.S.
dc.contributor.authorToubar S.S.
dc.contributor.authorHelmy M.I.
dc.contributor.otherAnalytical Chemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherHelwan University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Pharmaceutical Analytical Chemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts
dc.contributor.other6 October City
dc.contributor.otherEgypt; Analytical Chemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:54Z
dc.date.available2020-01-09T20:40:54Z
dc.date.issued2018
dc.descriptionScopus
dc.description.abstractA facile, fast and specific method based on liquid chromatography�tandem mass spectrometry (LC�MS/MS) for the simultaneous quantitation of paracetamol, chlorzoxazone and aceclofenac in human plasma was developed and validated. Sample preparation was achieved by liquid�liquid extraction. The analysis was performed on a reversed-phase C18 HPLC column (5 ?m, 4.6 � 50 mm) using acetonitrile�10 mM ammonium formate pH 3.0 (65:35, v/v) as the mobile phase where atrovastatin was used as an internal standard. A very small injection volume (3 ?L) was applied and the run time was 2.0 min. The detection was carried out by electrospray positive and negative ionization mass spectrometry in the multiple-reaction monitoring mode. The developed method was capable of determining the analytes over the concentration ranges of 0.03�30.0, 0.015�15.00 and 0.15�15.00 ?g/mL for paracetamol, chlorzoxazone and aceclofenac, respectively. Intraday and interday precisions (as coefficient of variation) were found to be ?12.3% with an accuracy (as relative error) of �5.0%. The method was successfully applied to a pharmacokinetic study of the three analytes after being orally administered to six healthy volunteers. Copyright � 2018 John Wiley & Sons, Ltd.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=23942&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1002/bmc.4232
dc.identifier.doiPubMed ID 29505100
dc.identifier.issn2693879
dc.identifier.otherhttps://doi.org/10.1002/bmc.4232
dc.identifier.otherPubMed ID 29505100
dc.identifier.urihttps://t.ly/2dPbO
dc.language.isoEnglishen_US
dc.publisherJohn Wiley and Sons Ltden_US
dc.relation.ispartofseriesBiomedical Chromatography
dc.relation.ispartofseries32
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectUniversity of Modern Sciences and Arts
dc.subjectMSA University
dc.subjectaceclofenacen_US
dc.subjectchlorzoxazoneen_US
dc.subjectHPLC�MS/MSen_US
dc.subjecthuman plasmaen_US
dc.subjectparacetamolen_US
dc.subjectpharmacokinetic studyen_US
dc.subjectaceclofenacen_US
dc.subjectacetonitrileen_US
dc.subjectammonium formateen_US
dc.subjectatorvastatinen_US
dc.subjectchlorzoxazoneen_US
dc.subjectparacetamolen_US
dc.subjectaceclofenacen_US
dc.subjectchlorzoxazoneen_US
dc.subjectdiclofenacen_US
dc.subjectparacetamolen_US
dc.subjectArticleen_US
dc.subjectcontrolled studyen_US
dc.subjectdrug blood levelen_US
dc.subjecthumanen_US
dc.subjecthuman experimenten_US
dc.subjectliquid chromatography-mass spectrometryen_US
dc.subjectliquid liquid extractionen_US
dc.subjectnormal humanen_US
dc.subjectsingle drug doseen_US
dc.subjectanalogs and derivativesen_US
dc.subjectblooden_US
dc.subjectchemistryen_US
dc.subjectlimit of detectionen_US
dc.subjectliquid chromatographyen_US
dc.subjectmaleen_US
dc.subjectproceduresen_US
dc.subjectreproducibilityen_US
dc.subjectstatistical modelen_US
dc.subjecttandem mass spectrometryen_US
dc.subjectAcetaminophenen_US
dc.subjectChlorzoxazoneen_US
dc.subjectChromatography, Liquiden_US
dc.subjectDiclofenacen_US
dc.subjectHumansen_US
dc.subjectLimit of Detectionen_US
dc.subjectLinear Modelsen_US
dc.subjectMaleen_US
dc.subjectReproducibility of Resultsen_US
dc.subjectTandem Mass Spectrometryen_US
dc.titleLiquid chromatography–tandem MS/MS method for simultaneous quantification of paracetamol, chlorzoxazone and aceclofenac in human plasma: An application to a clinical pharmacokinetic studyen_US
dc.typeArticleen_US
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