Liquid chromatography–tandem MS/MS method for simultaneous quantification of paracetamol, chlorzoxazone and aceclofenac in human plasma: An application to a clinical pharmacokinetic study
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Date
2018
Authors
Mohamed D.
Hegazy M.A.
Elshahed M.S.
Toubar S.S.
Helmy M.I.
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
John Wiley and Sons Ltd
Series Info
Biomedical Chromatography
32
32
Scientific Journal Rankings
Abstract
A facile, fast and specific method based on liquid chromatography�tandem mass spectrometry (LC�MS/MS) for the simultaneous quantitation of paracetamol, chlorzoxazone and aceclofenac in human plasma was developed and validated. Sample preparation was achieved by liquid�liquid extraction. The analysis was performed on a reversed-phase C18 HPLC column (5 ?m, 4.6 � 50 mm) using acetonitrile�10 mM ammonium formate pH 3.0 (65:35, v/v) as the mobile phase where atrovastatin was used as an internal standard. A very small injection volume (3 ?L) was applied and the run time was 2.0 min. The detection was carried out by electrospray positive and negative ionization mass spectrometry in the multiple-reaction monitoring mode. The developed method was capable of determining the analytes over the concentration ranges of 0.03�30.0, 0.015�15.00 and 0.15�15.00 ?g/mL for paracetamol, chlorzoxazone and aceclofenac, respectively. Intraday and interday precisions (as coefficient of variation) were found to be ?12.3% with an accuracy (as relative error) of �5.0%. The method was successfully applied to a pharmacokinetic study of the three analytes after being orally administered to six healthy volunteers. Copyright � 2018 John Wiley & Sons, Ltd.
Description
Scopus
Keywords
October University for Modern Sciences and Arts, جامعة أكتوبر للعلوم الحديثة والآداب, University of Modern Sciences and Arts, MSA University, aceclofenac, chlorzoxazone, HPLC�MS/MS, human plasma, paracetamol, pharmacokinetic study, aceclofenac, acetonitrile, ammonium formate, atorvastatin, chlorzoxazone, paracetamol, aceclofenac, chlorzoxazone, diclofenac, paracetamol, Article, controlled study, drug blood level, human, human experiment, liquid chromatography-mass spectrometry, liquid liquid extraction, normal human, single drug dose, analogs and derivatives, blood, chemistry, limit of detection, liquid chromatography, male, procedures, reproducibility, statistical model, tandem mass spectrometry, Acetaminophen, Chlorzoxazone, Chromatography, Liquid, Diclofenac, Humans, Limit of Detection, Linear Models, Male, Reproducibility of Results, Tandem Mass Spectrometry