Evaluation of multivariate calibration models with different pre-processing and processing algorithms for a novel resolution and quantitation of spectrally overlapped quaternary mixture in syrup

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Date

2016

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Journal ISSN

Volume Title

Type

Article

Publisher

Elsevier

Series Info

Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
154

Abstract

A novel approach for the resolution and quantitation of severely overlapped quaternary mixture of carbinoxamine maleate (CAR), pholcodine (PHL), ephedrine hydrochloride (EPH) and sunset yellow (SUN) in syrup was demonstrated utilizing different spectrophotometric assisted multivariate calibration methods. The applied methods have used different processing and pre-processing algorithms. The proposed methods were partial least squares (PLS), concentration residuals augmented classical least squares (CRACLS), and a novel method; continuous wavelet transforms coupled with partial least squares (CWT-PLS). These methods were applied to a training set in the concentration ranges of 40-100 ?g/mL, 40-160 ?g/mL, 100-500 ?g/mL and 8-24 ?g/mL for the four components, respectively. The utilized methods have not required any preliminary separation step or chemical pretreatment. The validity of the methods was evaluated by an external validation set. The selectivity of the developed methods was demonstrated by analyzing the drugs in their combined pharmaceutical formulation without any interference from additives. The obtained results were statistically compared with the official and reported methods where no significant difference was observed regarding both accuracy and precision. 2015 Elsevier B.V. All rights reserved.

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Scopus

Keywords

Carbinoxamine, Continuous wavelet transforms coupled with partial least squares (CWT-PLS), Cyrinol� syrup, Ephedrine, Multivariate calibration methods, Pholcodine, Algorithms, Calibration, Drug products, Mixtures, Multivariable systems, Wavelet transforms, Carbinoxamine, Ephedrine, Multivariate calibration methods, Partial least square (PLS), Pholcodine, Least squares approximations, azo compound, carbinoxamine, codeine, ephedrine, FD & C Yellow No. 6, morpholine derivative, pholcodine, pyridine derivative, algorithm, analogs and derivatives, calibration, evaluation study, least square analysis, multivariate analysis, procedures, spectrophotometry, Algorithms, Azo Compounds, Calibration, Codeine, Ephedrine, Least-Squares Analysis, Morpholines, Multivariate Analysis, Pyridines, Spectrophotometry

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