Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorWasfi R.
dc.contributor.authorAbd El-Rahman O.A.
dc.contributor.authorZafer M.M.
dc.contributor.authorAshour H.M.
dc.contributor.otherDepartment of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA)
dc.contributor.otherGiza
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAl-Azhar University (Girls)
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAhram Canadian University (ACU)
dc.contributor.otherGiza
dc.contributor.otherEgypt; Department of Biological Sciences
dc.contributor.otherCollege of Arts and Sciences
dc.contributor.otherUniversity of South Florida St. Petersburg
dc.contributor.otherSt. Petersburg
dc.contributor.otherFL
dc.contributor.otherUnited States; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:59Z
dc.date.available2020-01-09T20:40:59Z
dc.date.issued2018
dc.descriptionScopus
dc.descriptionMSA Google Scholar
dc.description.abstractStreptococcus mutans contributes significantly to dental caries, which arises from homoeostasic imbalance between host and microbiota. We hypothesized that Lactobacillus sp. inhibits growth, biofilm formation and gene expression of Streptococcus mutans. Antibacterial (agar diffusion method) and antibiofilm (crystal violet assay) characteristics of probiotic Lactobacillus sp. against Streptococcus mutans (ATCC 25175) were evaluated. We investigated whether Lactobacillus casei (ATCC 393), Lactobacillus reuteri (ATCC 23272), Lactobacillus plantarum (ATCC 14917) or Lactobacillus salivarius (ATCC 11741) inhibit expression of Streptococcus mutans genes involved in biofilm formation, quorum sensing or stress survival using quantitative real-time polymerase chain reaction (qPCR). Growth changes (OD600) in the presence of pH-neutralized, catalase-treated or trypsin-treated Lactobacillus sp. supernatants were assessed to identify roles of organic acids, peroxides and bacteriocin. Susceptibility testing indicated antibacterial (pH-dependent) and antibiofilm activities of Lactobacillus sp. against Streptococcus mutans. Scanning electron microscopy revealed reduction in microcolony formation and exopolysaccharide structural changes. Of the oral normal flora, L.salivarius exhibited the highest antibiofilm and peroxide-dependent antimicrobial activities. All biofilm-forming cells treated with Lactobacillus sp. supernatants showed reduced expression of genes involved in exopolysaccharide production, acid tolerance and quorum sensing. Thus, Lactobacillus sp. can inhibit tooth decay by limiting growth and virulence properties of Streptococcus mutans. � 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=15852&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1111/jcmm.13496
dc.identifier.doiPubMed ID 29316223
dc.identifier.issn15821838
dc.identifier.otherhttps://doi.org/10.1111/jcmm.13496
dc.identifier.otherPubMed ID 29316223
dc.identifier.urihttps://t.ly/OXMmM
dc.language.isoEnglishen_US
dc.publisherBlackwell Publishing Inc.en_US
dc.relation.ispartofseriesJournal of Cellular and Molecular Medicine
dc.relation.ispartofseries22
dc.subjectbiofilmen_US
dc.subjectdental cariesen_US
dc.subjectprobiotic Lactobacillusen_US
dc.subjectStreptococcus mutansen_US
dc.subjectbacteriocinen_US
dc.subjectexopolysaccharideen_US
dc.subjectgamma interferonen_US
dc.subjectinterleukin 10en_US
dc.subjectprobiotic agenten_US
dc.subjectbacterial polysaccharideen_US
dc.subjectbacteriocinen_US
dc.subjectcatalaseen_US
dc.subjectgamma interferonen_US
dc.subjectIL10 protein, humanen_US
dc.subjectinterleukin 10en_US
dc.subjectperoxideen_US
dc.subjectprobiotic agenten_US
dc.subjecttrypsinen_US
dc.subjectagar diffusionen_US
dc.subjectantibiotic sensitivityen_US
dc.subjectantimicrobial activityen_US
dc.subjectArticleen_US
dc.subjectbacterial growthen_US
dc.subjectbiofilmen_US
dc.subjectcolony formationen_US
dc.subjectcolony forming uniten_US
dc.subjectcontrolled studyen_US
dc.subjectenzyme linked immunosorbent assayen_US
dc.subjectgene expressionen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectLactobacillusen_US
dc.subjectLactobacillus caseien_US
dc.subjectLactobacillus plantarumen_US
dc.subjectLactobacillus reuterien_US
dc.subjectLactobacillus salivariusen_US
dc.subjectnonhumanen_US
dc.subjectnormal humanen_US
dc.subjectpriority journalen_US
dc.subjectquorum sensingen_US
dc.subjectreal time polymerase chain reactionen_US
dc.subjectscanning electron microscopyen_US
dc.subjectStreptococcus mutansen_US
dc.subjectzone of inhibitionen_US
dc.subjectantibiosisen_US
dc.subjectbiofilmen_US
dc.subjectbiosynthesisen_US
dc.subjectchemistryen_US
dc.subjectculture mediumen_US
dc.subjectcytologyen_US
dc.subjectdrug effecten_US
dc.subjectgeneticsen_US
dc.subjectgrowth, development and agingen_US
dc.subjectimmunologyen_US
dc.subjectmetabolismen_US
dc.subjectmicrobial sensitivity testen_US
dc.subjectmicrobiologyen_US
dc.subjectmononuclear cellen_US
dc.subjectpathogenicityen_US
dc.subjectpHen_US
dc.subjectprimary cell cultureen_US
dc.subjectStreptococcus mutansen_US
dc.subjectAntibiosisen_US
dc.subjectBacteriocinsen_US
dc.subjectBiofilmsen_US
dc.subjectCatalaseen_US
dc.subjectCulture Mediaen_US
dc.subjectHumansen_US
dc.subjectHydrogen-Ion Concentrationen_US
dc.subjectInterferon-gammaen_US
dc.subjectInterleukin-10en_US
dc.subjectLactobacillus caseien_US
dc.subjectLactobacillus plantarumen_US
dc.subjectLactobacillus reuterien_US
dc.subjectLactobacillus salivariusen_US
dc.subjectLeukocytes, Mononuclearen_US
dc.subjectMicrobial Sensitivity Testsen_US
dc.subjectPeroxidesen_US
dc.subjectPolysaccharides, Bacterialen_US
dc.subjectPrimary Cell Cultureen_US
dc.subjectProbioticsen_US
dc.subjectQuorum Sensingen_US
dc.subjectStreptococcus mutansen_US
dc.subjectTrypsinen_US
dc.titleProbiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutansen_US
dc.typeArticleen_US
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