Polymorphisms in gap junction proteins and their role in predisposition of acute myocardial infarction in Egyptians

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorEl Tahry F.A.
dc.contributor.authorHashad I.M.
dc.contributor.authorRahman M.F.A.
dc.contributor.authorGad M.Z.
dc.contributor.otherClinical Biochemistry Unit
dc.contributor.otherFaculty of Pharmacy and Biotechnology
dc.contributor.otherGerman University in Cairo
dc.contributor.otherCairo
dc.contributor.otherEgypt; Biochemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Science and Arts
dc.contributor.other6th of October City
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:20Z
dc.date.available2020-01-09T20:41:20Z
dc.date.issued2017
dc.descriptionScopus
dc.description.abstractBackground: Connexin (Cx) proteins are the building blocks of gap junctions. Among these, Cx37 and Cx40 are expressed on vascular system and reported to have cardioprotective role. Linking polymorphisms in genes coding for Cx and coronary artery disease (CAD) risk showed conflicting results in different populations. None has been studied before in Egyptians. Therefore, the aims of this study were to investigate the influence of Cx37 C1019T and Cx40 A71G polymorphisms on the predisposition of acute myocardial infarction (AMI) in Egyptians, to study linkage disequilibrium (LD) and combined effects of single nucleotide polymorphisms (SNPs) and to correlate the genotypes with sVCAM-1 serum levels. Methods: Total of 201 Egyptian subjects were recruited for the study. They were divided into 104 AMI patients and 97 healthy controls. Genotypes for each participant were determined using a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Serum sVCAM-1was measured by ELISA. Results: Allele frequencies for both Cx37 and Cx40 were not significantly different between AMI and Controls (p=0.93 and p=0.26 respectively). Moreover, studying the dominant and recessive models concluded that none of the genotypes was a risk factor. Both SNPs were not in LD (R2=0.0027). Serum analysis showed higher levels of sVCAM-1 in AMI patients (p<0.0001). sVCAM-1 levels were not significantly different among SNPs (Cx37; p=0.244 and Cx40; p=0.266). � 2017 Bentham Science Publishers.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=15581&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.2174/1389201018666171002125432
dc.identifier.doiPubMed ID 28969560
dc.identifier.issn13892010
dc.identifier.otherhttps://doi.org/10.2174/1389201018666171002125432
dc.identifier.otherPubMed ID 28969560
dc.identifier.urihttps://t.ly/mb8jA
dc.language.isoEnglishen_US
dc.publisherBentham Science Publishers B.V.en_US
dc.relation.ispartofseriesCurrent Pharmaceutical Biotechnology
dc.relation.ispartofseries18
dc.subjectA71G polymorphismen_US
dc.subjectC1019T polymorphismen_US
dc.subjectConnexin 37en_US
dc.subjectConnexin 40en_US
dc.subjectEgyptiansen_US
dc.subjectGap junctionsen_US
dc.subjectMyocardial infarctionen_US
dc.subjectSVCAM-1en_US
dc.subjectcholesterolen_US
dc.subjectgap junction proteinen_US
dc.subjecttriacylglycerolen_US
dc.subjectvascular cell adhesion molecule 1en_US
dc.subjectconnexin 37en_US
dc.subjectconnexin 40en_US
dc.subjectgap junction proteinen_US
dc.subjectvascular cell adhesion molecule 1en_US
dc.subjectacute heart infarctionen_US
dc.subjectadulten_US
dc.subjectArticleen_US
dc.subjectcontrolled studyen_US
dc.subjectDNA purificationen_US
dc.subjectenzyme linked immunosorbent assayen_US
dc.subjectfemaleen_US
dc.subjectgene frequencyen_US
dc.subjectgene linkage disequilibriumen_US
dc.subjectgenetic predispositionen_US
dc.subjectgenetic variabilityen_US
dc.subjectgenotypeen_US
dc.subjecthumanen_US
dc.subjectmajor clinical studyen_US
dc.subjectmaleen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectrestriction fragment length polymorphismen_US
dc.subjectsingle nucleotide polymorphismen_US
dc.subjectblooden_US
dc.subjectcase control studyen_US
dc.subjectEgypten_US
dc.subjectgenetic predispositionen_US
dc.subjectgeneticsen_US
dc.subjectheart infarctionen_US
dc.subjectmiddle ageden_US
dc.subjectrisk factoren_US
dc.subjectAdulten_US
dc.subjectCase-Control Studiesen_US
dc.subjectConnexinsen_US
dc.subjectEgypten_US
dc.subjectFemaleen_US
dc.subjectGene Frequencyen_US
dc.subjectGenetic Predisposition to Diseaseen_US
dc.subjectHumansen_US
dc.subjectLinkage Disequilibriumen_US
dc.subjectMaleen_US
dc.subjectMiddle Ageden_US
dc.subjectMyocardial Infarctionen_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjectPolymorphism, Single Nucleotideen_US
dc.subjectRisk Factorsen_US
dc.subjectVascular Cell Adhesion Molecule-1en_US
dc.titlePolymorphisms in gap junction proteins and their role in predisposition of acute myocardial infarction in Egyptiansen_US
dc.typeArticleen_US
dcterms.isReferencedByMese, G., Richard, G., White, T.W., Gap junctions: Basic structure and function (2007) J. Invest. Dermatol, 127 (11), pp. 2516-2524; Zhou, J.Z., Jiang, J.X., Gap junction and hemichannel-independent actions of connexins on cell and tissue functions--an update (2014) FEBS Lett, 588 (8), pp. 1186-1192; Bosco, D., Haefliger, J.A., Meda, P., Connexins: Key mediators of endocrine function (2011) Physiol. Rev, 91 (4), pp. 1393-1445; Meens, M.J., Pfenniger, A., Kwak, B.R., Risky communication in atherosclerosis and thrombus formation (2012) Swiss Med. Wkly, 142, pp. w13553; Scheckenbach, K.E., Crespin, S., Kwak, B.R., Chanson, M., Connexin channel-dependent signaling pathways in inflammation (2011) J. Vasc. Res, 48 (2), pp. 91-103; Chanson, M., Kwak, B.R., Connexin37: A potential modifier gene of inflammatory disease (2007) J. Mol. Med (Berl), 85 (8), pp. 787-795; Pfenniger, A., Van Der Laan, S.W., Foglia, B., Dunoyer-Geindre, S., Haefliger, J.A., Winnik, S., Mach, F., Kwak, B.R., Lack of association between connexin40 polymorphisms and coronary artery disease (2012) Atherosclerosis, 222 (1), pp. 148-153; Wong, C.W., Christen, T., Roth, I., Chadjichristos, C.E., Derouette, J.P., Foglia, B.F., Chanson, M., Kwak, B.R., Connexin37 protects against atherosclerosis by regulating monocyte adhesion (2006) Nat. Med, 12 (8), pp. 950-954; Angelillo-Scherrer, A., Fontana, P., Burnier, L., Roth, I., Sugamele, R., Brisset, A., Morel, S., Kwak, B.R., Connexin 37 limits thrombus propensity by downregulating platelet reactivity (2011) Circulation, 124 (8), pp. 930-939; Chadjichristos, C.E., Scheckenbach, K.E., Van Veen, T.A., Richani, S.M.Z., De Wit, C., Yang, Z., Roth, I., Kwak, B.R., Endothelial-specific deletion of connexin40 promotes atherosclerosis by increasing CD73- dependent leukocyte adhesion (2010) Circulation, 121 (1), pp. 123-131; Wen, D., Du, X., Nie, S.P., Dong, J.Z., Ma, C.S., Association of Connexin37 C1019T with myocardial infarction and coronary artery disease: A meta-analysis (2014) Exp. Gerontol, 58, pp. 203-207; Pfenniger, A., Derouette, J.P., Verma, V., Lin, X., Foglia, B., Coombs, W., Roth, I., Delmar, M., Gap junction protein Cx37 interacts with endothelial nitric oxide synthase in endothelial cells (2010) Arterioscler. Thromb. Vasc. Biol, 30 (4), pp. 827-834; Severs, N.J., Dupont, E., Coppen, S.R., Halliday, D., Inett, E., Baylis, D., Rothery, S., Remodelling of gap junctions and connexin expression in heart disease (2004) Biochim. Biophys. Acta, 1662 (1-2), pp. 138-148; Morel, S., Braunersreuther, V., Chanson, M., Bouis, D., Rochemont, V., Foglia, B., Pelli, G., Kwak, B.R., Endothelial Cx40 limits myocardial ischaemia/reperfusion injury in mice (2014) Cardiovasc. Res, 102 (2), pp. 329-337; Schmidt, K., Kaiser, F.J., Erdmann, J., Wit, C., Two polymorphisms in the Cx40 promoter are associated with hypertension and left ventricular hypertrophy preferentially in men (2015) Clin. Experiment. Hypert, 37 (7), pp. 580-586; Okugawa, Y., Miki, C., Toiyama, Y., Koike, Y., Inoue, Y., Kusunoki, M., Serum level of soluble vascular cell adhesion molecule 1 is a valuable prognostic marker in colorectal carcinoma (2009) Dis. Colon Rectum, 52 (7), pp. 1330-1336; Ballantyne, C.M., Entman, M.L., Soluble adhesion molecules and the search for biomarkers for atherosclerosis (2002) J. Amer. Heart Assoc, 106, pp. 766-767; Li, Y.Y., Qian, Y., Zhou, C.W., Lack of association between the C1019T gene polymorphism and coronary artery disease in a Chinese population: Meta-analysis of 2,206 subjects (2013) Biomed. Rep, 1 (3), pp. 464-468; Listi, F., Lio, D., Russo, M., Colonna-Romano, G., Caruso, M., Hoffmann, E., Caruso, C., Association between C1019T polymorphism of connexin37 and acute myocardial infarction: A study in patients from Sicily (2005) Int. J. Cardiol, 102 (2), pp. 269-271; Han, Y., Xi, S., Zhang, X., Yan, C., Yang, Y., Kang, J., Association of connexin 37 gene polymorphisms with risk of coronary artery disease in northern Han Chinese (2008) Cardiology, 110 (4), pp. 260-265; Wong, C.W., Christen, T., Pfenniger, A., James, R.W., Kwak, B.R., Do allelic variants of the connexin37 1019 gene polymorphism differentially predict for coronary artery disease and myocardial infarction? (2007) Atherosclerosis, 191 (2), pp. 355-361; Horan, P.G., Allen, A.R., Patterson, C.C., Spence, M.S., McGlinchey, P.G., McKeown, P.P., The connexin 37 gene polymorphism and coronary artery disease in Ireland (2006) Heart, 92 (3), pp. 395-396; Wirka, R.C., Gore, S., Van Wagoner, D.R., Arking, D.E., Lubitz, S.A., Lunetta, K.L., Benjamin, E.J., Smith, J.D., A common connexin-40 gene promoter variant affects connexin-40 expression in human atria and is associated with atrial fibrillation (2011) Circ. Arrhythm. Electrophysiol, 4 (1), pp. 87-93; Seifi, M., Fallah, S., Ghasemi, A., Aghajani, H., Razaghi, M., Danaei, N., Mutations of the connexin 37 and 40 gap-junction genes in patients with acute myocardial infarction (2013) Clin. Lab, 59 (3-4), pp. 343-348; Ni, X., Valente, J., Azevedo, M.H., Pato, M.T., Pato, C.N., Kennedy, J.L., Connexin 50 gene on human chromosome 1q21 is associated with schizophrenia in matched case control and familybased studies (2007) J. Med. Genet, 44 (8), pp. 532-536; Collings, A., Islam, M.S., Juonala, M., Rontu, R., K�h�nen, M., Hutri-K�h�nen, N., Laitinen, T., Lehtim�ki, T.J., Associations between connexin37 gene polymorphism and markers of subclinical atherosclerosis: The cardiovascular risk in young Finns study (2007) Atherosclerosis, 195 (2), pp. 379-384; Wu, Z., Lou, Y., Jin, W., Liu, Y., Lu, L., Chen, Q., Zhang, R., The Connexin37 gene C1019T polymorphism and risk of coronary artery disease: A meta-analysis (2014) Arch. Med. Res, 45 (1), pp. 21-30; Feng, Y., Sun, J., Wang, L., Hou, X.L., A study of the association between the connexin 40 rs35594137 polymorphism and atrial fibrillation in Xinjiang Chinese Han and Uygur populations (2015) Genet. Mol. Res, 14 (4), pp. 15705-15712; Bossowska, A., Kiersnowska-Rogowska, B., Bossowski, A., Galar, B., Sowi?ski, P., Assessment of serum levels of adhesion molecules (SICAM-1, sVCAM-1, sE-selectin) in stable and unstable angina and acute myocardial infarction (2003) Przegl. Lek, 60 (7), pp. 445-450; Hwang, S.J., Ballantyne, C.M., Sharrett, A.R., Smith, L.C., Davis, C.E., Gottom, A.M., Jr., Boerwinkle, E., Circulating adhesion molecules VCAM-1, ICAM-1, and E-selectin in carotid atherosclerosis and incident coronary heart disease cases: The Atherosclerosis Risk In Communities (ARIC) study (1997) Circulation, 96 (12), pp. 4219-4925; Nakai, K., Itoh, C., Kawazoe, K., Miura, Y., Sotoyanagi, H., Hotta, K., Itoh, T., Hiramori, K., Concentration of soluble vascular cell adhesion molecule-1 (VCAM-1) correlated with expression of VCAM-1 mRNA in the human atherosclerotic aorta (1995) Coron. Artery Dis, 6 (6), pp. 497-502
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