Simultaneous Determination of Five Coccidiostats in Veterinary Powders, Feed Premixes, and Baby Food by Micellar Electrokinetic Chromatography: Application to Chicken Tissues and Liver

Abstract

A new, simple, and reliable micellar electrokinetic chromatographic method was developed and validated for the simultaneous determination of amprolium, ethopabate, diaveridine, sulphadimidine, and sulphaquinoxaline. The separation was achieved using 50�mM tris buffer (pH 8.5) with 50�mM SDS and 15% acetonitrile (v/v) at 28�kV and the components were detected at 200�nm. Different factors affecting the electrophoretic mobility of the five investigated drugs were studied and optimized. Method validation showed that calibration plots were linear within the range from 0.5 to 100�?g/mL with a correlation coefficient > 0.998. Intraday and interday precision and accuracy evaluated by relative standard deviation were lower than 2%. The limits of detection were in the ranges of 0.02 to 0.07�?g/mL. The new method with simple sample pretreatment based on aqueous methanol extraction has been successfully applied for analysis of these drugs in powder preparations, feed premixes, baby food, chicken tissues, and liver samples with the recoveries of 97�101%. The present method is suitable and favorable for the analysis of the five coccidiostats drugs on account of its cost effectiveness, simplicity, rapidity, and sensitivity. � 2018, Springer Science+Business Media, LLC, part of Springer Nature.