Phenolic profile, anti-inflammatory, antinociceptive, anti-ulcerogenic and hepatoprotective activities of Pimenta racemosa leaves

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorMoharram F.A.-E.
dc.contributor.authorAl-Gendy A.A.
dc.contributor.authorEl-Shenawy S.M.
dc.contributor.authorIbrahim B.M.
dc.contributor.authorZarka M.A.
dc.contributor.otherHelwan University
dc.contributor.otherDepartment of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo
dc.contributor.other11795
dc.contributor.otherEgypt; Zagazig University
dc.contributor.otherDepartment of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherZagazig
dc.contributor.other44519
dc.contributor.otherEgypt; National Research Center
dc.contributor.otherDepartment of Pharmacology
dc.contributor.otherDokki
dc.contributor.otherCairo
dc.contributor.other12622
dc.contributor.otherEgypt; Modern Sciences and Arts University
dc.contributor.otherDepartment of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.other6 October City
dc.contributor.otherEgypt; Modern University for Technology and Information
dc.contributor.otherDepartment of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:54Z
dc.date.available2020-01-09T20:40:54Z
dc.date.issued2018
dc.descriptionScopus
dc.description.abstractBackground: Pimenta racemosa tree has many traditional uses where its leaves are used as herbal tea for treatment of flatulence, gastric disorder, osteoarthritis, colds and fever in addition to its analgesic and anti-inflammatory activities. So, this study aimed to isolate phenolic constituents of 80% aqueous methanol extract (AME) of leaves and evaluate its biological activities. Methods: The defatted AME was chromatographed and structures of the isolated compounds were elucidated using UV, NMR spectroscopy and UPLC-ESI-MS analysis. Antioxidant activity was investigated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity. Anti-inflammatory activity was evaluated using carrageenan - induced paw oedema, while antinociceptive activity was determined by chemical and thermal stimuli. Anti-ulcerogenic effect of AME against gastric damage induced by ethanol in Wister male albino rats was evaluated. Also, hepatoprotective activity was investigated through determination of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) following oral administration of paracetamol. Both of Anti-ulcerogenic and hepatoprotective activities (125, 250 and 500 mg/kg b.wt.) were supported by histopathological examinations. Results: Gallic acid (1), methyl gallate (2), avicularin (3), quercetin 3-O-?-D-arbinopyranoside (4), quercetin 3-O-?-D-glucopyranoside (5), quercetrin (6), cynaroside (7), strictinin (8), castalagin (9), grandinin (10) quercetin (11) and ellagic acid (12) were isolated. AME showed significant radical scavenging activity (SC50 = 4.6 ?g/mL), promising anti-inflammatory effect through inhibition of oedema and antinociceptive activity by reduction in number of writhes after acetic acid injection and prolongation of reaction time towards the thermal stimulus. AME reduced the gastric mucosal lesions compared with ethanol control and ranitidine groups, ALT at the three doses and AST only at 125 and 250 mg/kg b.wt., when compared with paracetamol group. The results were confirmed by histopathological studies. Conclusion:P. racemosa leaves are rich in phenolic compounds and showed significant biological activities. � 2018 The Author(s).en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=34441&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1186/s12906-018-2260-3
dc.identifier.doiPubMed ID 29976187
dc.identifier.issn14726882
dc.identifier.otherhttps://doi.org/10.1186/s12906-018-2260-3
dc.identifier.otherPubMed ID 29976187
dc.identifier.urihttps://t.ly/LZK2Y
dc.language.isoEnglishen_US
dc.publisherBioMed Central Ltd.en_US
dc.relation.ispartofseriesBMC Complementary and Alternative Medicine
dc.relation.ispartofseries18
dc.subjectAnti-inflammatoryen_US
dc.subjectAnti-ulcerogenicen_US
dc.subjectAntinociceptiveen_US
dc.subjectHepatoprotectiveen_US
dc.subjectMyrtaceaeen_US
dc.subjectPhenolic compoundsen_US
dc.subjectPimenta racemosaen_US
dc.subject1,1 diphenyl 2 picrylhydrazylen_US
dc.subjectacetic aciden_US
dc.subjectacetylsalicylic aciden_US
dc.subjectalanine aminotransferaseen_US
dc.subjectalcoholen_US
dc.subjectaspartate aminotransferaseen_US
dc.subjectavicularineen_US
dc.subjectcastalaginen_US
dc.subjectellagic aciden_US
dc.subjectflavonoiden_US
dc.subjectgallic aciden_US
dc.subjectgallic acid methyl esteren_US
dc.subjectgrandininen_US
dc.subjectindometacinen_US
dc.subjectluteolin 7 glucosideen_US
dc.subjectmethanolen_US
dc.subjectparacetamolen_US
dc.subjectphenol derivativeen_US
dc.subjectPimenta racemosa extracten_US
dc.subjectplant extracten_US
dc.subjectplant medicinal producten_US
dc.subjectquercetinen_US
dc.subjectquercetin 3 o beta dextro arabinopyranosideen_US
dc.subjectquercetin 3 o beta dextro glucopyranosideen_US
dc.subjectquercetrinen_US
dc.subjectranitidineen_US
dc.subjectsilymarinen_US
dc.subjectstrictininen_US
dc.subjecttramadolen_US
dc.subjectunclassified drugen_US
dc.subjectanalgesic agenten_US
dc.subjectantiinflammatory agenten_US
dc.subjectphenol derivativeen_US
dc.subjectplant extracten_US
dc.subjectprotective agenten_US
dc.subjectadulten_US
dc.subjectalanine aminotransferase blood levelen_US
dc.subjectanalgesic activityen_US
dc.subjectanimal experimenten_US
dc.subjectanimal modelen_US
dc.subjectanimal tissueen_US
dc.subjectantiinflammatory activityen_US
dc.subjectantioxidant activityen_US
dc.subjectantiulcer activityen_US
dc.subjectaqueous solutionen_US
dc.subjectArticleen_US
dc.subjectaspartate aminotransferase blood levelen_US
dc.subjectcarrageenan-induced paw edemaen_US
dc.subjectchromatographyen_US
dc.subjectcontrolled studyen_US
dc.subjectDPPH radical scavenging assayen_US
dc.subjectdrug dose comparisonen_US
dc.subjectdrug isolationen_US
dc.subjectdrug structureen_US
dc.subjectelectrospray mass spectrometryen_US
dc.subjectethanol-induced gastric ulceren_US
dc.subjectfemaleen_US
dc.subjectheat stressen_US
dc.subjecthistopathologyen_US
dc.subjecthot plate testen_US
dc.subjectliver diseaseen_US
dc.subjectliver protectionen_US
dc.subjectmaleen_US
dc.subjectmouseen_US
dc.subjectnonhumanen_US
dc.subjectnuclear magnetic resonance spectroscopyen_US
dc.subjectnumber of writhesen_US
dc.subjectphysical chemistryen_US
dc.subjectphytochemistryen_US
dc.subjectPimentaen_US
dc.subjectPimenta racemosaen_US
dc.subjectplant leafen_US
dc.subjectraten_US
dc.subjectresponse timeen_US
dc.subjectstomach mucosa lesionen_US
dc.subjectultra performance liquid chromatographyen_US
dc.subjectultraviolet spectroscopyen_US
dc.subjectWistar raten_US
dc.subjectwrithing testen_US
dc.subjectanimalen_US
dc.subjectanimal behavioren_US
dc.subjectchemistryen_US
dc.subjectdrug effecten_US
dc.subjectedemaen_US
dc.subjectliveren_US
dc.subjectpathologyen_US
dc.subjectpathophysiologyen_US
dc.subjectplant leafen_US
dc.subjectstomachen_US
dc.subjectAnalgesicsen_US
dc.subjectAnimalsen_US
dc.subjectAnti-Inflammatory Agentsen_US
dc.subjectBehavior, Animalen_US
dc.subjectEdemaen_US
dc.subjectFemaleen_US
dc.subjectLiveren_US
dc.subjectMaleen_US
dc.subjectPhenolsen_US
dc.subjectPimentaen_US
dc.subjectPlant Extractsen_US
dc.subjectPlant Leavesen_US
dc.subjectProtective Agentsen_US
dc.subjectRatsen_US
dc.subjectRats, Wistaren_US
dc.subjectStomachen_US
dc.titlePhenolic profile, anti-inflammatory, antinociceptive, anti-ulcerogenic and hepatoprotective activities of Pimenta racemosa leavesen_US
dc.typeArticleen_US
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