COLONIZATION OF MULTI-DRUG RESISTANT (MDR) ACINETOBACTER BAUMANNII ISOLATED FROM TERTIARY HOSPITALS IN EGYPT AND THE POSSIBLE ROLE OF THE OUTER MEMBRANE PROTEIN (OMPA)
| dc.Affiliation | October University for modern sciences and Arts (MSA) | |
| dc.contributor.author | Ismail Fahmy, Lamiaa | |
| dc.contributor.author | Magdy Amin, Heba | |
| dc.contributor.author | Fahmy Mohamed, Aly | |
| dc.contributor.author | Hashem, Abdel Gawad | |
| dc.date.accessioned | 2020-02-22T08:48:37Z | |
| dc.date.available | 2020-02-22T08:48:37Z | |
| dc.date.issued | 2018 | |
| dc.description | MSA Google Scholar | en_US |
| dc.description.abstract | This study aimed to investigate the virulence (adhesion and invasion) of clinically isolated multidrug resistant (MDR) A. baumannii on different mammalian cell lines. It also determined the role of the outer membrane protein (OmpA) extracted from such isolates in cytotoxicity to these cell lines. In this study, hundred thirty- seven clinical isolates were obtained from intensive care units (ICUs) at tertiary hospitals in Cairo, Egypt and were identified by API system. Identification was confirmed by polymerase chain reaction (PCR) amplification of the oxa-51 gene. Based on their susceptibility to different antimicrobial agents, tested by disc diffusion method, isolates were classified into different groups. Representative isolates of each group were assayed for adherence and invasion on HEp-2, A549 and Hela cells. OmpA was then extracted from selected strains. The cytotoxicity and caspase activation of the extracted OmpA was determined on different cell lines. The isolates were found to harbor the oxa-51 gene. Multidrug resistance was accompanied by higher binding capabilities on HEp-2 and A549 cells than Hela cells. A relation between cytotoxicity and Caspase activation was suspected among the isolates. These results suggested that apoptosis induced by OmpA of MDR A. baumannii might be attributed to caspase activation, though other mechanism could not be ruled out. In conclusion, A. baumannii has the ability to cause adhesion and invasion on respiratory cell other than other types of cells. OmpA has a vital role in the pathogenesis as it causes apoptosis to the respiratory cells. | en_US |
| dc.description.sponsorship | INTERNATIONAL RESEARCH JOURNAL OF PHARMACY | en_US |
| dc.identifier.citation | 1. Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, Bonomo RA. Global challenge of multidrug-resistant Acinetobacter baumannii. Antimicrobial agents and chemotherapy. 2007;51(10):3471-84. https://doi.org/10.1128 /AAC.01464-06 PMid:17646423 PMCid:PMC2043292 2. Mohajeri P, Farahani A, Feizabadi MM, Ketabi H, Abiri R, Najafi F. Antimicrobial susceptibility profiling and genomic diversity of Acinetobacter baumannii isolates: A study in western Iran. Iranian journal of microbiology. 2013;5(3):195- 202. PMid:24475323 PMCid:PMC3895554 3. Smith SG, Mahon V, Lambert MA, Fagan RP. A molecular Swiss army knife: OmpA structure, function and expression. FEMS microbiology letters. 2007;273(1):1-11. https:// doi.org/10.1111/j.1574-6968.2007.00778.x PMCid:PMC3644883 4. Choi CH, Lee EY, Lee YC, Park TI, Kim HJ, Hyun SH, et al. Outer membrane protein 38 of Acinetobacter baumannii localizes to the mitochondria and induces apoptosis of epithelial cells. Cellular microbiology. 2005;7(8):1127-38. https://doi.org/10.1111/j.1462-5822.2005.00538.x PMid:16008580 5. Rumbo C, Tomas M, Fernandez Moreira E, Soares NC, Carvajal M, Santillana E, et al. The Acinetobacter baumannii Omp33-36 porin is a virulence factor that induces apoptosis and modulates autophagy in human cells. Infection and immunity. 2014;82(11):4666-80. https://doi.org/10.1128/IAI .02034-14 PMid:25156738 PMCid:PMC4249306 6. Turton JF, Woodford N, Glover J, Yarde S, Kaufmann ME, Pitt TL. Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. Journal of clinical microbiology. 2006;44(8):2974-6. https://doi.org/10.1128/JCM.01021-06 PMid:16891520 PMCid:PMC1594603 7. CLSI. Performance standard for antimicrobial susceptibility testing. Twenty-First Informational Supplement. 2015;M100-S21:31(1). Lamiaa Ismail Fahmy et al. Int. Res. J. Pharm. 2018, 9 (7) 110 8. Brossard KA, Campagnari AA. The Acinetobacter baumannii biofilm-associated protein plays a role in adherence to human epithelial cells. Infection and immunity. 2012;80(1):228-33. https://doi.org/10.1128/IAI.05913-11 PMid:22083703 PMCid:PMC3255684 9. Jitendraa Vashistt VT, Arti Kapil and Rajeswari R Moganty. Differential expression of Outer membrane proteins in early stages of meropenem-resistance in Acinetobacter baumannii. JIOMICS. 2011;1(2):280-6. 10. Kim YH, Cho K, Yun SH, Kim JY, Kwon KH, Yoo JS, et al. Analysis of aromatic catabolic pathways in Pseudomonas putida KT 2440 using a combined proteomic approach: 2- DE/MS and cleavable isotope-coded affinity tag analysis. Proteomics. 2006;6(4):1301-18. https://doi.org/10.1002/ pmic.200500329 PMid:16470664 11. Fotakis G, Timbrell JA. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicology letters. 2006;160(2):171-7. https://doi.org/ 10.1016/j.toxlet.2005.07.001 PMid:16111842 12. Lee JC, Koerten H, van den Broek P, Beekhuizen H, Wolterbeek R, van den Barselaar M, et al. Adherence of Acinetobacter baumannii strains to human bronchial epithelial cells. Research in microbiology. 2006;157(4):360- 6. https://doi.org/10.1016/j.resmic.2005.09.011 PMid:16326077 13. Gaddy JA, Actis LA. Regulation of Acinetobacter baumannii biofilm formation. Future microbiology. 2009;4(3):273-8. https://doi.org/10.2217/fmb.09.5 PMid:19327114 PMCid:PMC2724675 14. McConnell MJ, Pachon J. Expression, purification, and refolding of biologically active Acinetobacter baumannii OmpA from Escherichia coli inclusion bodies. Protein expression and purification. 2011;77(1):98-103. https:// doi.org/10.1016/j.pep.2010.11.019 PMid:21130880 15. Doi Y, Murray GL, Peleg AY. Acinetobacter baumannii: evolution of antimicrobial resistance-treatment options. Seminars in respiratory and critical care medicine. 2015;36(1):85-98. https://doi.org/10.1055/s-0034-1398388 PMid:25643273 PMCid:PMC4465586 16. Asadollahi P, Akbari M, Soroush S, Taherikalani M, Asadollahi K, Sayehmiri K, et al. Antimicrobial resistance patterns and their encoding genes among Acinetobacter baumannii strains isolated from burned patients. Burns : journal of the International Society for Burn Injuries. 2012;38(8):1198-203. https://doi.org/10.1016/ j.burns.2012.04.008 PMid:22579564 17. Rafei R, Dabboussi F, Hamze M, Eveillard M, Lemarie C, Gaultier MP, et al. Molecular analysis of Acinetobacter baumannii strains isolated in Lebanon using four different typing methods. PloS one. 2014;9(12):e115969. https://doi.org/10.1371/journal.pone.0115969 PMid:25541711 PMCid:PMC4277430 18. Mortensen E, Trivedi KK, Rosenberg J, Cody SH, Long J, Jensen BJ, et al. Multidrug-resistant Acinetobacter baumannii infection, colonization, and transmission related to a longterm care facility providing subacute care. Infection control and hospital epidemiology. 2014;35(4):406-11. https://doi.org/10.1086/675612 PMid:24602946 19. Krzyminska S, Frackowiak H, Kaznowski A. Acinetobacter calcoaceticus-baumannii complex strains induce caspasedependent and caspase-independent death of human epithelial cells. Current microbiology. 2012;65(3):319-29. https://doi.org/10.1007/s00284-012-0159-7 PMid:22684803 PMCid:PMC3401494 20. Lee HW, Koh YM, Kim J, Lee JC, Lee YC, Seol SY, et al. Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 2008;14(1):49-54. https://doi.org/10.1111/j.1469-0691.2007.01842.x PMid:18005176 21. Choi CH, Hyun SH, Lee JY, Lee JS, Lee YS, Kim SA, et al. Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity. Cellular microbiology. 2008;10(2):309-19. PMid:17760880 22. Jin JS, Kwon SO, Moon DC, Gurung M, Lee JH, Kim SI, Lee JC. Acinetobacter baumannii secretes cytotoxic outer membrane protein A via outer membrane vesicles. PloS one. 2011;6(2):e17027. https://doi.org/10.1371/journal.pone.0017027 PMid:21386968 PMCid:PMC3046175 23. Zhivotovsky B. Apoptosis, necrosis and between. Cell cycle. 2004;3(1):64-6. https://doi.org/10.4161/cc.3.1.606 PMid:14657668 24. Bratu S, Landman D, Martin DA, Georgescu C, Quale J. Correlation of antimicrobial resistance with beta-lactamases, the OmpA-like porin, and efflux pumps in clinical isolates of Acinetobacter baumannii endemic to New York City. Antimicrobial agents and chemotherapy. 2008;52(9):2999- 3005. https://doi.org/10.1128/AAC.01684-07 PMid:185912 75 PMCid:PMC2533509 | en_US |
| dc.identifier.doi | https://doi.org/10.7897/2230-8407.097133 | |
| dc.identifier.issn | 2230-8407 | |
| dc.identifier.other | https://doi.org/10.7897/2230-8407.097133 | |
| dc.identifier.uri | https://t.ly/7OMn2 | |
| dc.language.iso | en | en_US |
| dc.publisher | International Research Journal of Pharmacy | en_US |
| dc.relation.ispartofseries | INTERNATIONAL RESEARCH JOURNAL OF PHARMACY;Volume: 9 Issue: 7 Pages: 103-110 | |
| dc.subject | University of Adhesion, Invasion, A. baumannii, OmpA, cytotoxicity, caspase | en_US |
| dc.title | COLONIZATION OF MULTI-DRUG RESISTANT (MDR) ACINETOBACTER BAUMANNII ISOLATED FROM TERTIARY HOSPITALS IN EGYPT AND THE POSSIBLE ROLE OF THE OUTER MEMBRANE PROTEIN (OMPA) | en_US |
| dc.type | Article | en_US |
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