Somatic embryogenesis and plant regeneration from callus and suspension cultures of Iphiona mucronata (Forssk)
Date
2013
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
European Scientific Journal
Series Info
European Scientific Journal;Volume: 9 Issue: 27 Pages: 37-49
Doi
Scientific Journal Rankings
Abstract
A protocol was designed for plant regeneration of Iphiona mucronata
from embryogenic callus via somatic embryogenesis to enable micro
propagation of this endangered plant. The embryogenic callus was induced
using seedling cultured for nine months on Murashig and Skoog medium
(MS) supplemented with 0.1 mg l-1 naphthalene acetic acid (NAA), 0.1 mg l1 kinetin (Kn) and 5 mg l-1 ascorbic acid and incubated in the dark followed
by growing on hormone free medium. Transfer of developed embryos to MS
medium supplemented with 0.5 mg l-1 kinetin induced shoot formation. Four
treatments were further tried for plant development by using indole acetic
acid (IAA) or indole butyric acid (IBA) alone or in combination with kinetin.
The results showed that 2 mg l-1 IAA was the best for in vitro plantlet
regeneration. Embryogenic suspension was induced by transfer of
embryogenic callus to liquid medium having the same composition followed
by hormone free medium where different stages of embryos were monitored.
Shoots were developed upon transfer to liquid medium supplemented with
0.5 mg l-1 Kn. However, no further development appeared upon transfer to
semi solid medium containing different phytohormones. Embryogenic callus
showed the highest phenolic contents when compared with embryogenic
suspension, regenerated plantlets and the parent plant while flavonoids were
detected only in embryogenic callus.
Description
MSA Google Scholar
Keywords
University of Iphiona mucronata, callus and suspension culture, somatic embryogenesis, plant regeneration
Citation
Ahmed, A. A. & Mabry, T. J. (1987). Flavonoids of Iphiona scabra. Phytochemistry, 26, 1517-1518. Ahmed, A. A.; Melek, F. R.; Seif El-Din, A. A. & Mabry, T. J. (1988). Polysulphated flavonoids from Iphiona mucronata. Rev. Latinoamer Quim., 19 (3), 107-109. Al-Gendy, A. A.; El-Gindi, O. D. & Bakr, R. O. (2008). Production of flavonoids in callus cultures of Iphiona mucronata, Astraceae. Egypt. J. Biomed. Sci., 28, 142-150. Al-Khayri, J. M. & Al-Bahrany, A. M. (2001). In vitro micropropagation of Citrus aurantifolia (lime). Curr. Sci, 81(9, 10), 1242-1246. Amoo, S. O. & Ayisire, B. E. (2005). Induction of callus and somatic embryogenesis from cotyledon explants of Parkia biglobosa (Jacq.) Benth. Afr. J. Biotechnol., 4 (1), 68-71. Anderberg, A. (1985). The genus Iphiona (Compositae-Inuleae). Nord J Bot, 5, 169-194. Antognoni, F.; Zheng, S.; Pagnucco, C.; Baraldi, R.; Poli, F. & Biondi, S. (2007). Induction of flavonoid production by UV-B radiation in Passiflora quadrangularis callus cultures. Fitoterapia, 78, 345–352. Beruto, M.; Curir, P. & Debergh, P. (1996). Callus growth and somatic embryogenesis in thalamus tissue of Ranunculus asiaticus L. cultivated in vitro: Cytokinin effect and phenol metabolism. In Vitro Cell Dev Biol – Plant, 32 (3), 154-160. Bhojwani, S. S. & Razdan, M. K. (1996). Plant tissue culture: theory and practical, a revised edition. Elsevier science, Netherlands, 148–150. Carman, J. G. (1990). Embryogenic cells in plant tissue cultures: occurrence and behavior. In Vitro Cell Dev Biol, 26, 746-753. Cockell, C. S. & Knowland, J. (1999). Ultraviolet radiation screening compounds. Biol Rev, 74, 311–345. Godoy-Hernández, G. & Vázquez-Flota, F. (2006). Growth measurements estimation of cell division and cell expansion. In: Loyola-Vargas VM, Vázquez-Flota F (ed) “ Plant Cell Culture Protocols”. 2nd edn. Humana Press Inc. Hrubcová, M; Cvikrová, M. & Eder, J. (1994). Peroxidase activities and contents of phenolic acids in embryogenic and non embryogenic alfalfa cell suspension. Biol Plantarum, 36 (2), 175-182. European Scientific Journal September 2013 edition vol.9, No.27 ISSN: 1857 – 7881 (Print) e - ISSN 1857- 7431 48 Kouakou, T. H.; Waffo-Téguo, P.; Kouadio, Y. J.; Valls, J.; Richard, T.; Decendit, A. & Mérillon, J. M. (2007). Phenolic compounds and somatic embryogenesis in cotton (Gossypium hirsutum L.). Plant Cell Tiss Organ Cult, 90, 25-29. Kosalec, I.; Bakmaz, M.; Pepeljnjak, S. & Vladimir-Knezevic, S. (2004). Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acat pharma, 54, 65-72. Lucchesini, M.; Bertoli, A.; Mensuali-Sodi, A. & Pistelli, L. (2009). Establishment of in vitro tissue cultures from Echinacea angustifolia D.C. adult plants for the production of phytochemical compounds. Sci Hort 122 (3), 484-490. Luo, J.; Jia, J.; Gu, Y. & Liu, J. (1999). High frequency somatic embryogenesis and plant regeneration in callus cultures of Astragalus adsurgens Pall. Plant Sci, 143, 93-99. May, R. A. & Trigiano, R. N. (1991). Somatic embryogenesis and plant regeneration from leaves of Dendranthema grandiflora. J Am Soc Hort Sci, 116 (2), 366-371. Merxmuller, H.; Leins, P. & Roesseler, H. (1977). Inuleae: systematic review. In: Heywood JB, Harborne JB, Turner BL (ed) The Biology and Chemistry of Compositae. Academic press, New York, pp 590-593. Monnier, M. (1990). Induction of embryogenesis in suspension culture. In: Pollard JW, Walker JM (ed). Methods in molecular biology. Plant cell and tissue culture. The Humana Press. Vol. 6, 149-157. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant, 15(3), 473-497. Naik, P. M. & Murthy, H. N. (2010). Somatic embryogenesis and plant regeneration from cell suspension culture of niger (Guizotia abyssinica Cass.). Acta Physiol Plant, 32, 75–79. Narasimhulu, S. B. & Reddy, G. M. (1983). Plantlet regeneration from different callus cultures of Arachis hypogaea L. Plant Sci Lett, 31(2-3), 157- 163. Noor, S.; Ali, G. M.; Rashid, U.; Arshad, M.; Ali, S. & Zafar, Y. (2009). Optimization of callus induction and regeneration system for Pakistani wheat cultivars Kohsar and Khyber-87. Afr. J. Biotechnol., 8 (20), 5565-5569. Parsaeimehr, A.; Sargsyan, E. & Javidnia, K. (2010). A comparative study of the antibacterial, antifungal and antioxidant activity and total content of phenolic compounds of cell cultures and wild plants of three endemic species of Ephedra. Molecules, 15(3), 1668-1678. Pescador, R.; Kerbauy, G. B.; Viviani, D. & Kraus, J. E. (2008). Anomalous somatic embryos in Acca sellowiana (O. Berg) Burret (Myrtaceae). Revista Brasil Bot, 31 (1), 155-164. European Scientific Journal September 2013 edition vol.9, No.27 ISSN: 1857 – 7881 (Print) e - ISSN 1857- 7431 49 Sellappan, S. & Akoh, C. C. (2002). Flavonoids and antioxidant capacity of Georgia-grown Vidalia onions. J Agric Food Chem 50, 5338-5342. Shohael, A. M.; Hahn, E. J. & Paek, K. Y. (2007). Somatic embryogenesis and secondary metabolite production through bioreactor culture of siberian ginseng (Eleutherococcus senticosus). Acta Hort. (ISHS) 764, 181-186. Verpoorte, R.; Van der Heijden, R.; ten Hoopen, H. J. G. & Memmelink, G. (1998). Metabolic engineering for the improvement of plant secondary metabolite production. Plant Tiss. Cult. Biotechnol, 4, 3 -19. Yantcheva, A.; Vlahova, M. & Antanassov, A. (1998). Direct somatic embryogenesis and plant regeneration of carnation (Dianthus caryophyllus L.). Plant Cell Rep, 18, 148–153. Zahran, M. A. & Willis, A. J. (2009). The vegetation of Egypt, 2nd edition, Springer.