Tecoma stans: Alkaloid profile and antimicrobial activity

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Date

2019

Journal Title

Journal ISSN

Volume Title

Type

Article

Publisher

Wolters Kluwer Medknow Publications

Series Info

Journal of Pharmacy and Bioallied Sciences
11

Abstract

Aim: Tecoma stans (L.) Kunth is a promising species in the trumpet creeper family Bignoniaceae. This study aimed at showing the antibacterial and antifungal potentials of T. stans methanolic leaf extract (TSME) correlated to its phytoconstituents. Materials and Methods: The antimicrobial potential of TSME was evaluated using agar diffusion method. The main alkaloids were separated on silica gel column and identified using nuclear magnetic resonance spectral analysis. Molecular docking was performed for the isolated compounds against MurD ligase, penicillin-binding protein, and dihydropteroate synthase enzyme to rationalize the observed antibacterial effect. Results and Discussion: TSME showed significant antibacterial effect against all tested microorganisms with comparable minimum inhibitory concentration (MIC) to the ampicillin and gentamicin with MIC values ranging between 0.98 and 1.95 ?g/mL, in addition to a promising antifungal effect when compared to amphotericin with MIC values 3.9 and 15.63 ?g/mL for Aspergillus flavus and Candida albicans, respectively. Several alkaloids were separated, purified, and identified as tecostanine, 4-OH tecomanine, 5-hydroxyskytanthine, and tecomanine, which were previously isolated from T. stans. The docking study showed that the alkaloids bind in a similar fashion to the co-crystallized ligands of the crystal structures of MurD ligase. The binding poses and scores in the case of penicillin-binding protein and dihydropteroate synthase did not match the co-crystallized ligands in their crystal structures. The in silico results suggest an antibacterial mechanism that involves the inhibition of MurD ligase. Conclusion: T. stans alkaloids could represent the basic skeleton for a powerful antimicrobial agent. � 2019 Journal of Pharmacy and Bioallied Sciences.

Description

Scopus

Keywords

October University for Modern Sciences and Arts, جامعة أكتوبر للعلوم الحديثة والآداب, University of Modern Sciences and Arts, MSA University, Alkaloid, antibacterial, molecular docking, Tecoma stans, 4 hydroxytecomanine, 5 hydroxyskytanthine, alkaloid, amphotericin B, ampicillin, antibiotic agent, antifungal agent, bacterial protein, dihydropteroate synthase, gentamicin, ligase, MurD protein, penicillin binding protein, plant extract, silica gel, Tecoma stans extract, tecomanine, tecostanine, unclassified drug, vancomycin, agar diffusion, antibacterial activity, antifungal activity, Article, Aspergillus flavus, Bacillus subtilis, Candida albicans, clinical evaluation, computer model, crystal structure, crystallization, drug isolation, drug mechanism, drug purification, enzyme inhibition, Klebsiella pneumoniae, minimum inhibitory concentration, molecular docking, nonhuman, nuclear magnetic resonance spectroscopy, phase separation, plant leaf, priority journal, Pseudomonas aeruginosa, Staphylococcus aureus, Tecoma stans

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