IL-10 and TGF-?: Roles in chondroprotective effects of Glucosamine in experimental Osteoarthritis?

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Date

2017

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Journal ISSN

Volume Title

Type

Article

Publisher

Elsevier B.V.

Series Info

Pathophysiology
24

Abstract

Objective Osteoarthritis (OA) is a complex disease of the whole joint. Glucosamine (GlcN) treatment may have a chondroprotective effect on OA. We investigated the mechanism of action of glucosamine treatment through interleukin-10 (Il-10) and transforming growth factor ?-1 (TGF ?-1). Methods Thirty male albino rats were used. A single intraarticular (i.a.) injection of 2�mg of Monosodium Iodoacetate (MIA) was injected into the knee joint of anesthetized rats. GlcN (50 or 100�mg/kg/day, p.o. for 2 month) was administered orally. Serum levels of Il-10 and TGF-?1 were determined by ELISA. Histopathological changes in treated and control joints were examined using hematoxylin-eosin (H & E) staining. Results The mean serum level of IL-10 significantly decreased in the OA group compared to control group (P value�<�0.0001). On the other hand, mean serum level of IL-10 significantly increased in GlcN treated groups when compared to the OA group (P value�<�0.0001). Serum level of TGF ?-1 was significantly elevated in OA group compared to control group (P value�<�0.0001). On the other hand, the mean serum level of TGF ?-1 was significantly decreased in the GlcN treated groups when compared to the OA group (P value�<�0.0001). Histopathological evaluation of GlcN treated groups showed different grades of healing, according to Osteoarthritis Research Society International (OARSI) grading system. Conclusion Our results showed that IL-10 and TGF-?1 possibly mediate GlcN chondroprotective effects in OA. Both serum biomarkers can be useful in the follow-up of articular cartilage damage in clinical settings. � 2017 Elsevier B.V.

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Scopus

Keywords

Glucosamine, IL-10�TGF-?, Osteoarthritis, biological marker, glucosamine, interleukin 10, transforming growth factor beta, animal experiment, animal model, Article, chondroprotection, controlled study, drug mechanism, enzyme linked immunosorbent assay, experimental osteoarthritis, healing, histopathology, male, nonhuman, protein blood level, rat

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