Molecular Characterization of Tunisian Barley (Hordeum Vulgare L.) Genotypes using Microsatellites (SSRs) Markers

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorChaabane, Ramzi
dc.contributor.authorEl Felah, Mouldi
dc.contributor.authorBen Salah, Hammadi
dc.contributor.authorBen Naceur, M’Barek
dc.contributor.authorAbdelly, Chedly
dc.contributor.authorRamla, Dalila
dc.contributor.authorNada, Ahmad
dc.contributor.authorSaker, Mahmoud
dc.date.accessioned2020-02-02T07:48:45Z
dc.date.available2020-02-02T07:48:45Z
dc.date.issued2009
dc.descriptionMSA Google Scholaren_US
dc.description.abstractHigh level of polymorphism make simple sequence repeats (SSRs) the molecular marker of choice for diversity analysis in plant species. In this study 18 simple sequence repeats (SSRs) markers were used to characterize six Tunisian barley varieties (Faïz, Manel, Martin, Rihane, Roho, and Tej) as well as six landraces from different growing regions in Tunisia (Djerba, Gabes, Jendouba, kairouan, Kebili, Kerkennah). Amplification of SSRs loci were obtained for 17 primer pairs and only 11 among them showed clear polymorphic patterns. These 11 primers produced a total of 31 alleles. The number of alleles per marker ranged from 1 to 5 with an average of 2.81 alleles per locus. The data generated by these 11 primers were sufficient to discriminate the analysed barley genotypes. The UPGMA cluster analysis of the similarity data grouped the 12 studied genotypes into two groups according to their spike type (two-row and six-row barley), except for Manel verity witch is a six row type but it clustered among the two row group. These results will be useful for barley germplasm management in terms of biodiversity protection and design of new crossesen_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=4400151716&tip=sid&clean=0
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dc.identifier.issn1450-216X
dc.identifier.urihttps://t.ly/p8rZ7
dc.language.isoenen_US
dc.publisherEuropean Journal of Scientific Researchen_US
dc.relation.ispartofseriesEuropean Journal of Scientific Research;Vol.36 No.1 (2009), pp.6-15
dc.subjectSimple Sequence Repeats (SSRs)en_US
dc.subjectbarleyen_US
dc.subjectvarietiesen_US
dc.subjectlandracesen_US
dc.subjectsimilarityen_US
dc.titleMolecular Characterization of Tunisian Barley (Hordeum Vulgare L.) Genotypes using Microsatellites (SSRs) Markersen_US
dc.typeArticleen_US

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