Molecular Characterization of Tunisian Barley (Hordeum Vulgare L.) Genotypes using Microsatellites (SSRs) Markers
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Date
2009
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
European Journal of Scientific Research
Series Info
European Journal of Scientific Research;Vol.36 No.1 (2009), pp.6-15
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Abstract
High level of polymorphism make simple sequence repeats (SSRs) the molecular
marker of choice for diversity analysis in plant species. In this study 18 simple sequence
repeats (SSRs) markers were used to characterize six Tunisian barley varieties (Faïz,
Manel, Martin, Rihane, Roho, and Tej) as well as six landraces from different growing
regions in Tunisia (Djerba, Gabes, Jendouba, kairouan, Kebili, Kerkennah). Amplification
of SSRs loci were obtained for 17 primer pairs and only 11 among them showed clear
polymorphic patterns. These 11 primers produced a total of 31 alleles. The number of
alleles per marker ranged from 1 to 5 with an average of 2.81 alleles per locus. The data
generated by these 11 primers were sufficient to discriminate the analysed barley
genotypes. The UPGMA cluster analysis of the similarity data grouped the 12 studied
genotypes into two groups according to their spike type (two-row and six-row barley),
except for Manel verity witch is a six row type but it clustered among the two row group.
These results will be useful for barley germplasm management in terms of biodiversity
protection and design of new crosses
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Keywords
Simple Sequence Repeats (SSRs), barley, varieties, landraces, similarity
Citation
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