Validated HPLC Method for Simultaneous Determination of Aripiprazole and Co-Administered Clonazepam in Spiked Human Plasma
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Date
2017
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Journal of Pharmaceutical and Applied Chemistry An International Journal
Series Info
J. Pharm. Appl. Chem;3, 1, 57-61
Scientific Journal Rankings
Abstract
A sensitive and selective high performance liquid chromatographic method was developed and validated for the
simultaneous quantification of Aripiprazole and co-administered Clonazepam in spiked human plasma. Aripiprazole,
Clonazepam and the internal standard Citalopram were extracted from plasma and analyzed on a reversed-phase C18
column using a mobile phase consisting of acetonitrile and 0.05M potassium dihydrogen orthophosphate (pH= 3.6) in a
ratio of (40: 60, v/v). The ultraviolet (UV) detection was at 220 nm. The method was linear over concentration range (20-
1000 ng mL-1
) for both drugs. The method is very simple and allows obtaining a very good recovery of the analytes.
Description
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Keywords
University of Aripiprazole, Clonazepam
Citation
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