Validated HPLC Method for Simultaneous Determination of Aripiprazole and Co-Administered Clonazepam in Spiked Human Plasma

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Date

2017

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Volume Title

Type

Article

Publisher

Journal of Pharmaceutical and Applied Chemistry An International Journal

Series Info

J. Pharm. Appl. Chem;3, 1, 57-61

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Abstract

A sensitive and selective high performance liquid chromatographic method was developed and validated for the simultaneous quantification of Aripiprazole and co-administered Clonazepam in spiked human plasma. Aripiprazole, Clonazepam and the internal standard Citalopram were extracted from plasma and analyzed on a reversed-phase C18 column using a mobile phase consisting of acetonitrile and 0.05M potassium dihydrogen orthophosphate (pH= 3.6) in a ratio of (40: 60, v/v). The ultraviolet (UV) detection was at 220 nm. The method was linear over concentration range (20- 1000 ng mL-1 ) for both drugs. The method is very simple and allows obtaining a very good recovery of the analytes.

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Keywords

University of Aripiprazole, Clonazepam

Citation

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