Investigating miRNA-661 and ATG4-B mRNA expression as potential biomarkers for hepatocellular carcinoma

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorAli M.A.
dc.contributor.authorMatboli M.
dc.contributor.authorEl-Khazragy N.
dc.contributor.authorSaber O.
dc.contributor.authorEl-Nakeep S.
dc.contributor.authorAbdelzaher H.M.
dc.contributor.authorShafei A.E.-S.
dc.contributor.authorMostafa R.
dc.contributor.otherDepartment of Biomedical Research
dc.contributor.otherArmed Forces College of Medicine (AFCM)
dc.contributor.otherCairo
dc.contributor.other11774
dc.contributor.otherEgypt; Department of Medical Biochemistry and Molecular Biology
dc.contributor.otherAin Shams Faculty of Medicine Research Center (Masri)11778
dc.contributor.otherEgypt; Department of Clinical Pathology
dc.contributor.otherOncology Diagnostic Unit
dc.contributor.otherFaculty of Medicine
dc.contributor.otherAin Shams University
dc.contributor.otherCairo
dc.contributor.other11778
dc.contributor.otherEgypt; Armed Forces College of Medicine (AFCM)
dc.contributor.otherCairo
dc.contributor.other11774
dc.contributor.otherEgypt; Hepatology and Gastroenterology Unit
dc.contributor.otherInternal Medicine Department
dc.contributor.otherFaculty of Medicine
dc.contributor.otherAin Shams University
dc.contributor.otherCairo
dc.contributor.other11778
dc.contributor.otherEgypt; Faculty of Biotechnology
dc.contributor.otherOctober University for Modern Sciences and Arts
dc.contributor.otherCairo
dc.contributor.other12585
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:58Z
dc.date.available2020-01-09T20:40:58Z
dc.date.issued2018
dc.descriptionScopus
dc.description.abstractAim: We aimed to examine the statistical association between serum expression of miRNA 661 (miR-661) and ATG-4B mRNA and hepatocellular carcinoma (HCC) based on in silico data analysis followed by clinical validation. Patients & methods: Quantitative reverse-transcriptase real-time PCR was used to examine the expression of miR-661 and ATG-4B mRNA in the sera of HCC patients versus control. Results: The expression of miR-661 and ATG-4B mRNA was positive in 97.14 and 77.14%, respectively, in HCC patients. The survival analysis showed that ATG-4B mRNA was an independent prognostic factor. Conclusion: Our data are the first report of its kind regarding the considerable clinical significance of miR-661 and ATG-4B mRNA in HCC patients. � 2018 2018 Future Medicine Ltd.en_US
dc.identifier.doihttps://doi.org/10.2217/bmm-2017-0273
dc.identifier.doiPubMedID29441798
dc.identifier.issn17520363
dc.identifier.otherhttps://doi.org/10.2217/bmm-2017-0273
dc.identifier.otherPubMedID29441798
dc.identifier.urihttps://t.ly/q2rjD
dc.language.isoEnglishen_US
dc.publisherFuture Medicine Ltd.en_US
dc.relation.ispartofseriesBiomarkers in Medicine
dc.relation.ispartofseries12
dc.subjectATG-4Ben_US
dc.subjectbioinformaticsen_US
dc.subjectbiomarkersen_US
dc.subjecthepatocellular carcinomaen_US
dc.subjectmiR-661en_US
dc.subjectprognosisen_US
dc.subjectalanine aminotransferaseen_US
dc.subjectbilirubinen_US
dc.subjectcysteine proteinaseen_US
dc.subjectmessenger RNAen_US
dc.subjectmicroRNAen_US
dc.subjectmicroRNA 661en_US
dc.subjectprotein ATG 4en_US
dc.subjectunclassified drugen_US
dc.subjectATG4B protein, humanen_US
dc.subjectautophagy related proteinen_US
dc.subjectcysteine proteinaseen_US
dc.subjectmessenger RNAen_US
dc.subjectmicroRNAen_US
dc.subjectMIRN661 microRNA, humanen_US
dc.subjecttumor markeren_US
dc.subjectadulten_US
dc.subjectalanine aminotransferase blood levelen_US
dc.subjectarea under the curveen_US
dc.subjectArticleen_US
dc.subjectATG4 B geneen_US
dc.subjectbilirubin blood levelen_US
dc.subjectcancer prognosisen_US
dc.subjectcancer stagingen_US
dc.subjectcancer survivalen_US
dc.subjectChild Pugh scoreen_US
dc.subjectdisease markeren_US
dc.subjectfemaleen_US
dc.subjectgeneen_US
dc.subjectgene expressionen_US
dc.subjecthumanen_US
dc.subjectliver cell carcinomaen_US
dc.subjectliver cirrhosisen_US
dc.subjectmajor clinical studyen_US
dc.subjectmaleen_US
dc.subjectprotein blood levelen_US
dc.subjectreceiver operating characteristicen_US
dc.subjectreverse transcription polymerase chain reactionen_US
dc.subjectsensitivity and specificityen_US
dc.subjectsurvival rateen_US
dc.subjectblooden_US
dc.subjectcase control studyen_US
dc.subjectgeneticsen_US
dc.subjectliver cell carcinomaen_US
dc.subjectliver tumoren_US
dc.subjectmetabolismen_US
dc.subjectmiddle ageden_US
dc.subjectpathologyen_US
dc.subjectprognosisen_US
dc.subjectupregulationen_US
dc.subjectArea Under Curveen_US
dc.subjectAutophagy-Related Proteinsen_US
dc.subjectBiomarkers, Tumoren_US
dc.subjectCarcinoma, Hepatocellularen_US
dc.subjectCase-Control Studiesen_US
dc.subjectCysteine Endopeptidasesen_US
dc.subjectFemaleen_US
dc.subjectHumansen_US
dc.subjectLiver Neoplasmsen_US
dc.subjectMaleen_US
dc.subjectMicroRNAsen_US
dc.subjectMiddle Ageden_US
dc.subjectNeoplasm Stagingen_US
dc.subjectPrognosisen_US
dc.subjectRNA, Messengeren_US
dc.subjectROC Curveen_US
dc.subjectUp-Regulationen_US
dc.titleInvestigating miRNA-661 and ATG4-B mRNA expression as potential biomarkers for hepatocellular carcinomaen_US
dc.typeArticleen_US
dcterms.isReferencedBy(2015) NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines R) Hepatobiliary Cancers. Version 22015, , National Comprehensive Cancer Network R; El-Tawdi, A.H.F., Matboli, M., El-Nakeep, S., Azazy, A.E.M., Abdel-Rahman, O., Association of long noncoding RNA and c-JUN expression in hepatocellular carcinoma (2016) Expert Rev. Gastroenterol. Hepatol., 10 (7), pp. 869-877; Chava, S., Chandra, P.K., Aydin, Y., Balart, L.A., Wu, T., Autophagy in hepatocellular carcinomas: From pathophysiology to therapeutic response (2016) Hepatic Med. Evid. Res., 8, pp. 9-20; Song, Y.-J., Zhang, S.-S., Guo, X.-L., Autophagy contributes to the survival of CD133+ liver cancer stem cells in the hypoxic and nutrient-deprived tumor microenvironment (2013) Cancer Lett., 339 (1), pp. 70-81; Ding, Z.-B., Shi, Y.-H., Zhou, J., Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma (2008) Cancer Res., 68 (22), pp. 9167-9175; Sui, X., Chen, R., Wang, Z., Autophagy and chemotherapy resistance: A promising therapeutic target for cancer treatment (2013) Cell Death Dis., 4, p. e838; Lee, Y.J., Jang, B.K., The role of autophagy in hepatocellular carcinoma (2015) Int. J. Mol. Sci., 16 (11), pp. 26629-26643; Li, J., Ouyang, L., Liu, B., Cheng, Y., Unraveling the roles of ATG4 proteases from autophagy modulation to targeted cancer therapy (2016) Cancer Lett., 373 (1), pp. 19-26; Cabrera, S., Maciel, M., Herrera, I., Essential role for the ATG4B protease and autophagy in bleomycin-induced pulmonary fibrosis (2015) Autophagy, 11 (4), pp. 670-684; Liu, P.-F., Leung, C.-M., Chang, Y.-H., ATG4B promotes colorectal cancer growth independent of autophagic flux (2014) Autophagy, 10 (8), pp. 1454-1465; Wu, S., Su, J., Qian, H., Guo, T., SLC27A4 regulate ATG4B activity and control reactions to chemotherapeutics-induced autophagy in human lung cancer cells (2016) Tumour Biol. J. Int. Soc. Oncodevelopmental Biol. Med., 37 (5), pp. 6943-6952; Zhao, H., Yang, M., Zhao, J., Wang, J., Zhang, Y., Zhang, Q., High expression of LC3B is associated with progression and poor outcome in triple-negative breast cancer (2013) Med. Oncol. Northwood Lond. Engl., 30 (1), p. 475; Kunanopparat, A., Kimkong, I., Palaga, T., Tangkijvanich, P., Sirichindakul, B., Hirankarn, N., Increased ATG5-ATG12 in hepatitis B virus-associated hepatocellular carcinoma and their role in apoptosis (2016) World J. Gastroenterol., 22 (37), pp. 8361-8374; Toshima, T., Shirabe, K., Matsumoto, Y., Autophagy enhances hepatocellular carcinoma progression by activation of mitochondrial-oxidation (2014) J. Gastroenterol., 49 (5), pp. 907-916; Chen, X., Ba, Y., Ma, L., Characterization of microRNAs in serum: A novel class of biomarkers for diagnosis of cancer and other diseases (2008) Cell Res., 18 (10), pp. 997-1006; Jing, Z., Han, W., Sui, X., Xie, J., Pan, H., Interaction of autophagy with microRNAs and their potential therapeutic implications in human cancers (2015) Cancer Lett., 356 (2), pp. 332-338; Gougelet, A., Colnot, S., Hepatocellular carcinoma diagnosis: Circulating microRNAs emerge as robust biomarkers (2016) Clin. Res. Hepatol. Gastroenterol., 40 (4), pp. 367-369; Singh, R., Ramasubramanian, B., Kanji, S., Chakraborty, A.R., Haque, S.J., Chakravarti, A., Circulating microRNAs in cancer: Hope or hype (2016) Cancer Lett., 381 (1), pp. 113-121; Hoffman, Y., Bublik, D.R., Pilpel, Y., Oren, M., MiR-661 downregulates both Mdm2 and Mdm4 to activate p53 (2014) Cell Death Differ., 21 (2), pp. 302-309; Kim, J.K., Kim, T.S., Basu, J., Jo, E.-K., MicroRNA in innate immunity and autophagy during mycobacterial infection (2017) Cell. Microbiol., 19 (1); Vetter, G., Saumet, A., Moes, M., MiR-661 expression in SNAI1-induced epithelial to mesenchymal transition contributes to breast cancer cell invasion by targeting Nectin-1 and StarD10 messengers (2010) Oncogene, 29 (31), pp. 4436-4448; Bui-Nguyen, T.M., Pakala, S.B., Sirigiri, D.R., Martin, E., Murad, F., Kumar, R., Stimulation of inducible nitric oxide by hepatitis B virus transactivator protein HBx requires MTA1 coregulator (2010) J. Biol. Chem., 285 (10), pp. 6980-6986; Zhu, T., Yuan, J., Wang, Y., Gong, C., Xie, Y., Li, H., MiR-661 contributed to cell proliferation of human ovarian cancer cells by repressing INPP5J expression (2015) Biomed. Pharmacother. Biomedecine Pharmacother., 75, pp. 123-128; Llovet, J.M., Br�, C., Bruix, J., Prognosis of hepatocellular carcinoma: The BCLC staging classification (1999) Semin. Liver Dis., 19 (3), pp. 329-338; Child, C.G., Turcotte, J.G., Surgery and portal hypertension (1964) Major Probl. Clin. Surg., 1, pp. 1-85; Pugh, R.N., Murray-Lyon, I.M., Dawson, J.L., Pietroni, M.C., Williams, R., Transection of the oesophagus for bleeding oesophageal varices (1973) Br. J. Surg., 60 (8), pp. 646-649; GenAtlas, , http://genatlas.medecine.univ-paris5.fr/; The Human Protein Atlas, , www.proteinatlas.org/; Autophagy Regulatory Network, , http://arn.elte.hu/; MicroRNA. Org-targets and Expression, , www.microrna.org/microrna/home.do; Paraskevopoulou, M.D., Georgakilas, G., Kostoulas, N., DIANA-microT web server v5. 0: Service integration into miRNA functional analysis workflows (2013) Nucleic Acids Res., 41, pp. W169-W173; Kanehisa, M., Furumichi, M., Tanabe, M., Sato, Y., Morishima, K., KEGG: New perspectives on genomes, pathways, diseases and drugs (2017) Nucleic Acids Res., 45 (D1), pp. D353-D361; Fleige, S., Pfaffl, M.W., RNA integrity and the effect on the real-time qRT-PCR performance (2006) Mol. Aspects Med., 27 (2-3), pp. 126-139; Livak, K.J., Schmittgen, T.D., Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method (2001) Methods San Diego Calif., 25 (4), pp. 402-408; Kwanten, W.J., Martinet, W., Michielsen, P.P., Francque, S.M., Role of autophagy in the pathophysiology of nonalcoholic fatty liver disease: A controversial issue (2014) World J. Gastroenterol., 20 (23), pp. 7325-7338; Li, Z., Liu, Y., Diao, H., Ma, J., Yao, Y., MiR-661 inhibits glioma cell proliferation, migration and invasion by targeting hTERT (2015) Biochem. Biophys. Res. Commun., 468 (4), pp. 870-876; Mizushima, N., Yoshimori, T., Levine, B., Methods in mammalian autophagy research (2010) Cell, 140 (3), pp. 313-326; Gupta, S., Bent, S., Kohlwes, J., Test characteristics of alpha-fetoprotein for detecting hepatocellular carcinoma in patients with hepatitis C. A systematic review and critical analysis (2003) Ann. Intern. Med., 139 (1), pp. 46-50; Valinezhad Orang, A., Safaralizadeh, R., Kazemzadeh-Bavili, M., Mechanisms of miRNA-Mediated gene regulation from common downregulation to mRNA-specific upregulation (2014) Int. J. Genomics, 2014, p. 970607; Xiao, M., Li, J., Li, W., MicroRNAs activate gene transcription epigenetically as an enhancer trigger (2016) RNA Biol., 14 (10), pp. 1326-1334; Li, L.-C., Okino, S.T., Zhao, H., Small dsRNAs induce transcriptional activation in human cells (2006) Proc. Natl Acad. Sci. USA, 103 (46), pp. 17337-17342; Place, R.F., Li, L.C., Pookot, D., Noonan, E.J., Dahiya, R., MicroRNA-373 induces expression of genes with complementary promoter sequences (2008) Proc. Natl Acad. Sci. USA, 105 (5), pp. 1608-1613
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