Circulating microRNAs as potential non-invasive biomarkers in pediatric patients with celiac disease

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorAmr K.S.
dc.contributor.authorBayoumi F.S.
dc.contributor.authorEissa E.
dc.contributor.authorAbu-Zekry M.
dc.contributor.otherMedical molecular genetics Department
dc.contributor.otherNational Research Centre
dc.contributor.otherCairo
dc.contributor.otherEgypt; Immunogenetics Department
dc.contributor.otherNational Research Centre
dc.contributor.otherCairo
dc.contributor.otherEgypt; Microbiology and Immunology Department
dc.contributor.otherMSA University
dc.contributor.otherEgypt; Immunogenetics Department
dc.contributor.otherNational Research Centre
dc.contributor.otherCairo
dc.contributor.otherEgypt; Abu El Reesh Children�s Hospital
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:43Z
dc.date.available2020-01-09T20:40:43Z
dc.date.issued2019
dc.descriptionScopus
dc.description.abstractCeliac disease is an enteropathy induced by ingestion of gluten triggering an immune response in genetically predisposed individuals. MiRNAs are small non-coding RNAs that have a role as regulators of gene expression at the post transcriptional level. The aim of this study is to evaluate the possibility of using circulating miRNAs as non-invasive biomarkers in pediatric patients with celiac disease. In addition, we examine the effect of a gluten-free diet on the expression of these miRNAs in serum of CD patients. The expression pattern of miR-21 and miR-31 was estimated in serum of 25 untreated CD patients (recently diagnosed), 25 treated CD patients (on gluten-free diet) and 20 healthy controls using qRT-PCR. Our results demonstrated the significant up-regulation of microRNA-21 in the untreated celiac patients in comparison with the treated group and healthy controls. Moreover, miR-31 expression was significantly under-expressed in the untreated celiac patients in comparison with the treated group and healthy controls. Furthermore, the results showed that miR-21 expression level was significantly positively correlated with the tTG IgA auto-antibodies. In conclusion, circulating miRNA-21 and miRNA-31 could serve as potential non-invasive biomarkers for pediatric CD patients. � 2019, EDRA S.p.A. All rights reserved.en_US
dc.identifier.doihttps://doi.org/10.23822/EurAnnACI.1764-1489.90
dc.identifier.doiPubMedID30983306
dc.identifier.issn17641489
dc.identifier.otherhttps://doi.org/10.23822/EurAnnACI.1764-1489.90
dc.identifier.otherPubMedID30983306
dc.identifier.urihttps://t.ly/YZZnJ
dc.language.isoEnglishen_US
dc.publisherElsevier B.V.
dc.publisherEDRA S.p.Aen_US
dc.relation.ispartofseriesEuropean Annals of Allergy and Clinical Immunology
dc.relation.ispartofseries51
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectUniversity of Modern Sciences and Arts
dc.subjectMSA University
dc.subjectAutoantibodiesen_US
dc.subjectBiomarkersen_US
dc.subjectCeliac diseaseen_US
dc.subjectGluten free dieten_US
dc.subjectMicroRNAen_US
dc.subjectimmunoglobulin A antibodyen_US
dc.subjectmicroRNA 21en_US
dc.subjectmicroRNA 31en_US
dc.subjectprotein glutamine gamma glutamyltransferaseen_US
dc.subjectadolescenten_US
dc.subjectArticleen_US
dc.subjectceliac diseaseen_US
dc.subjectchilden_US
dc.subjectchildhood diseaseen_US
dc.subjectclinical articleen_US
dc.subjectcontrolled studyen_US
dc.subjectdown regulationen_US
dc.subjectfemaleen_US
dc.subjectgene expressionen_US
dc.subjectgluten free dieten_US
dc.subjecthumanen_US
dc.subjectmaleen_US
dc.subjectreverse transcription polymerase chain reactionen_US
dc.subjectupregulationen_US
dc.titleCirculating microRNAs as potential non-invasive biomarkers in pediatric patients with celiac diseaseen_US
dc.typeArticleen_US
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