Circulating microRNAs as potential non-invasive biomarkers in pediatric patients with celiac disease

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Date

2019

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Volume Title

Type

Article

Publisher

Elsevier B.V.
EDRA S.p.A

Series Info

European Annals of Allergy and Clinical Immunology
51

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Abstract

Celiac disease is an enteropathy induced by ingestion of gluten triggering an immune response in genetically predisposed individuals. MiRNAs are small non-coding RNAs that have a role as regulators of gene expression at the post transcriptional level. The aim of this study is to evaluate the possibility of using circulating miRNAs as non-invasive biomarkers in pediatric patients with celiac disease. In addition, we examine the effect of a gluten-free diet on the expression of these miRNAs in serum of CD patients. The expression pattern of miR-21 and miR-31 was estimated in serum of 25 untreated CD patients (recently diagnosed), 25 treated CD patients (on gluten-free diet) and 20 healthy controls using qRT-PCR. Our results demonstrated the significant up-regulation of microRNA-21 in the untreated celiac patients in comparison with the treated group and healthy controls. Moreover, miR-31 expression was significantly under-expressed in the untreated celiac patients in comparison with the treated group and healthy controls. Furthermore, the results showed that miR-21 expression level was significantly positively correlated with the tTG IgA auto-antibodies. In conclusion, circulating miRNA-21 and miRNA-31 could serve as potential non-invasive biomarkers for pediatric CD patients. � 2019, EDRA S.p.A. All rights reserved.

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Scopus

Keywords

October University for Modern Sciences and Arts, جامعة أكتوبر للعلوم الحديثة والآداب, University of Modern Sciences and Arts, MSA University, Autoantibodies, Biomarkers, Celiac disease, Gluten free diet, MicroRNA, immunoglobulin A antibody, microRNA 21, microRNA 31, protein glutamine gamma glutamyltransferase, adolescent, Article, celiac disease, child, childhood disease, clinical article, controlled study, down regulation, female, gene expression, gluten free diet, human, male, reverse transcription polymerase chain reaction, upregulation

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