Browsing by Author "Othman M.S."

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Antitumor potential of berberine and cinnamic acid against solid ehrlich carcinoma in mice
(Bentham Science Publishers B.V., 2019) Almeer R.S.; Aref A.M.; Hussein R.A.; Othman M.S.; Moneim A.E.A.; Department of Zoology; College of Science; King Saud University; Riyadh; Saudi Arabia; Faculty of Biotechnology; October University for Modern Science and Arts (MSA); Giza; Egypt; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo; Egypt; Faculty of Preparatory year; University of Hail; Hail; Saudi Arabia
Background: Berberine and cinnamic acid are natural compounds that exhibit potent anticancer activities through distinct molecular mechanisms. Objective: In the present study, we aimed to investigate the proapoptotic potential of cinnamic acid and berberine in cancer cells by examining their effect on the expression of proapoptotic and antiapoptotic genes. Moreover, the effects of berberine and cinnamic acid on the antitumor activity of cisplatin were investigated in Ehrlich solid tumor-bearing mice. Methods: For the study, 90 male mice were inoculated intramuscularly with Ehrlich ascites tumor cells (2.5 � 106/mouse), and then on day 4, mice were randomly divided into six experimental groups (group 1-untreated Ehrlich solid tumor (EST), group 2-EST treated CDDP, group 3-EST treated CA, group 4-EST treated BER, group 5-EST treated CA + CDDP, and group 6-EST treated BER + CDDP). Result: The results showed that berberine and cinnamic acid significantly decreased tumor growth and tumor volume (- 74.8 and -75.5%, respectively) both as single agents and in combination with cisplatin. Moreover, both berberine and cinnamic acid increased the ratio of tumor growth inhibition (-91.5 and -92.6%, respectively), mean survival time (61.5 and 26 days, respectively), and percentage increase in lifespan (559 and 263%, respectively) of the treated mice. Our results also showed that both berberine and cinnamic acid-induced apoptosis by increasing the Bax/Bcl-2 ratio (74.1 and 45.1, respectively) and caspase-3 expression (14.3- and 11.6-fold increase, respectively). Additionally, berberine and cinnamic acid decreased oxidative stress markers, as shown by the decrease in lipid peroxidation and nitric oxide levels and an increase in reduced glutathione level. Conclusion: These results suggest that berberine and cinnamic acid have potential as antitumor and antioxidant agents derived from natural sources, which could be used alone or in combination with regular chemotherapeutic agents, such as cisplatin. These effects could be attributed to the proapoptotic activity of berberine and cinnamic acid. � 2019 Bentham Science Publishers.
Azadirachta indica attenuates cisplatin-induced nephrotoxicity and oxidative stress
(Hindawi Publishing Corporation, 2014) Abdel Moneim A.E.; Othman M.S.; Aref A.M.; Department of Biochemistry and Molecular Biology; Asturias Institute of Biotechnology; University of Oviedo; Oviedo; 33006; Spain; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo; 11795; Egypt; Department of Biochemistry and Molecular Biology; Faculty of Biotechnology; Modern Sciences and Arts University (MSA); Giza; 12111; Egypt; Department of Biological Science; Faculty of Dentistry; Modern Sciences and Arts University (MSA); Giza; 12111; Egypt
We investigated the effects of methanolic leaves extract of Azadirachta indica (MLEN, 500 mg/kg bwt) on cisplatin- (CP-) induced nephrotoxicity and oxidative stress in rats. CP (5 mg/kg bwt) was injected intraperitoneally and MLEN was given by gastric gavage for 5 days before or after CP injection. After 5 days of CP injection, CP-induced injury of the renal tissue was evidenced (i) as histopathological damage of the renal tissue, (ii) as increases in serum uric acid, urea, and creatinine, (iii) as increases in malondialdehyde (MDA) and nitric oxide (NO), (iv) as decreases in the level of glutathione and activities of superoxide dismutase, catalase, glutathione reductase, glutathione-S-transferase, and glutathione peroxidase, and (v) as increase in the expression of nuclear factor kappa B and apoptosis in kidney tissues. However, the oral administration of MLEN to CP-intoxicated rats for 5 days brought back MDA, NO production, and enzymatic and nonenzymatic antioxidants to near normalcy. Moreover, the histological observations evidenced that neem extract effectively rescues the kidney from CP-mediated oxidative damage. Furthermore, PCR results for caspase-3 and caspase-9 and Bax genes showed downregulation in MLEN treated groups. Therefore, Azadirachta indica can be considered a potential candidate for protection of nephrotoxicity induced by cisplatin. � 2014 Ahmed E. Abdel Moneim et al.
Ceratonia siliqua pod extract ameliorates Schistosoma mansoni-induced liver fibrosis and oxidative stress
(BioMed Central Ltd., 2016) Al-Olayan E.M.; El-Khadragy M.F.; Alajmi R.A.; Othman M.S.; Bauomy A.A.; Ibrahim S.R.; Abdel Moneim A.E.; King Saud University; Department of Zoology; Faculty of Science; Riyadh; Saudi Arabia; University of Helwan; Department of Zoology and Entomology; Faculty of Science; Cairo; Egypt; University of Hail; Faculty of Preparatory year; Hail; Saudi Arabia; October University for Modern Science and Arts (MSA); Faculty of Biotechnology; Giza; Egypt; Qassim University; Laboratory Sciences Department; College of Science and Arts; Al-Rass; Saudi Arabia; National Organization for Drug Control and Research (NODCAR); Molecular Drug Evaluation Department; Giza; Egypt
Background: Schistosomiasis is a prevalent parasitic disease found predominantly in tropical and sub-tropical areas of the developing world, with the second highest socioeconomic and public health burden despite strenuous control efforts. In the present study, we aimed to investigate the ameliorative effects of Ceratonia siliqua pod extract (CPE) on liver fibrosis and oxidative stress in mice infected with Schistosoma mansoni. Methods: The schistosomal hepatopathologic mouse model was established by tail immersion with schistosomal cercaria. The extract was given daily for 10 days beginning 42 days post-infection. Liver samples were obtained from mice sacrificed 9 weeks after infection. Liver histopathological changes were observed with hematoxylin-eosin and Masson trichrome staining. Results: Typical schistosomal hepatopathologic changes were induced in the untreated mice. However, the oral administration of CPE was effective in reducing worm number and the egg load in the liver. This treatment also decreased granuloma size and collagen deposition by inhibiting tissue inhibitor of metalloproteinases-2 (TIMP-2) expression. Schistosomal infection induced oxidative stress by increasing lipid peroxidation (LPO) and nitrite/nitrate (nitric oxide; NO) production along with concomitant decreases in glutathione (GSH) and various antioxidant enzymes, including superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. However, treatment of mice with CPE at 300 or 600 mg/kg inhibited LPO and NO production, increased GSH content, and restored the activities of the antioxidant enzymes compared with untreated infected mice. Furthermore, treatment with CPE inhibited apoptosis, as indicated by the reduced Bax expression in hepatic tissue. Conclusion: These data indicated that extracts from Ceratonia siliqua pods may play an important role in combating schistosomal hepatopathology and may inhibit granuloma formation and liver fibrosis through down-regulation of TIMP-2 expression. 2016 The Author(s).
Effect of Physalis peruviana L. on cadmium-induced testicular toxicity in rats
(MDPI AG, 2014) Othman M.S.; Nada A.; Zaki H.S.; Abdel Moneim A.E.; Faculty of Biotechnology; October University for Modern Science and Arts (MSA); Giza; Egypt; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo; Egypt; Department of Biochemistry and Molecular Biology; Asturias Institute of Biotechnology; University of Oviedo; Oviedo; Spain
Cadmium (Cd) stimulates the production of reactive oxygen species and causes tissue damage. We investigated here the protective effect of Physalis peruviana L. (family Solanaceae) against cadmium-induced testes toxicity in rats. Twenty-eight Wistar albino rats were used. They were divided into four groups (n=7). Group 1 was used as control. Group 2 was intraperitoneally injected with 6.5 mg/kg body weight (bwt) of cadmium chloride for 5 days. Group 3 was orally treated with 200 mg/kg bwt of methanolic extract of physalis (MEPh). Group 4 was pretreated with MEPh before cadmium for 5 days. Changes in body and testes weights were determined. Oxidative stress markers, antioxidant enzymes, and testosterone level were measured. Histopathological changes of testes were examined, and the immunohistochemical staining for the proapoptotic (caspase-3) protein was performed. The injection of cadmium caused a significant decrease in body weight, while a significant increase in testes weight and testes weight index was observed. Pretreatment with MEPh was associated with significant reduction in the toxic effects of Cd as shown by reduced testicular levels of malondialdehyde, nitric oxide, and caspase-3 expression and increased glutathione content, and the activities of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, and testosterone were also increased. Testicular histopathology showed that Cd produced an extensive germ cell apoptosis, and the pretreatment of MEPh in Cd-treated rats significantly reduced Cd-induced testicular damage. On the basis of the above results, it can be hypothesized that P. peruviana L. has a protective effect against cadmium-induced testicular oxidative stress and apoptosis in the rat. � 2014 Springer Science+Business Media.
Erratum to: Ceratonia siliqua pod extract ameliorates Schistosoma mansoni-induced liver fibrosis and oxidative stress [BMC Complementary and Alternative Medicine, 16, (2016) (434)] DOI: 10.1186/s12906-016-1389-1
(BioMed Central Ltd., 2017) Al-Olayan E.M.; El-Khadragy M.F.; Alajmi R.A.; Othman M.S.; Bauomy A.A.; Ibrahim S.R.; Moneim A.E.A.; King Saud University; Chair vaccines research of infectious diseases; Faculty of Science; Riyadh; Saudi Arabia; King Saud University; Department of Zoology; Faculty of Science; Riyadh; Saudi Arabia; University of Helwan; Department of Zoology and Entomology; Faculty of Science; Cairo; Egypt; University of Hail; Faculty of Preparatory year; Hail; Saudi Arabia; October University for Modern Science and Arts (MSA); Faculty of Biotechnology; Giza; Egypt; Qassim University; Laboratory Sciences Dept.; College of Science and Arts; Al-Rass; Saudi Arabia; Molecular Drug Evaluation Department; National Organization for Drug Control and Research (NODCAR); Giza; Egypt
Following publication of the original article [1] it wasbrought to our attention that the affiliations for thisarticle had been incorrectly presented. Please notethat the correct affiliations should be as follows:Ebtesam M. Al-Olayan 1,2 , Manal F. El-Khadragy 1,2,3, Reem A. Alajmi 1 , Mohamed S. Othman 4,5 , Amira A.Bauomy 3,6 , Shaimaa R. Ibrahim 7 and Ahmed E. Abde l Moneim 3* 1 Chair vaccines research of infectious diseases, Facultyof Science, King Saud University, Riyadh, and KSA 2 Department of Zoology, Faculty of Science, King SaudUniversity, Riyadh, KSA 3 Department of Zoology and Entomology, Faculty ofScience, University of Helwan, Cairo, Egypt 4 Faculty of Preparatory year, University of Hail, Hail,KSA 5 Faculty of Biotechnology, October University forModern Science and Arts (MSA), Giza, Egypt 6 Laboratory Sciences Dept., College of Science andArts, Qassim University, Al-Rass, KSA 7 Molecular Drug Evaluation Department, NationalOrganization for Drug Control and Research (NODCAR),Giza, EgyptPlease also note that the Acknowledgements sectionshould be updated to the following: "The authors extendtheir appreciation to the Deanship of Scientific Researchat King Saud University for funding the work throughthe research group project No. RGPVPP-074". � The Author(s).
Oleuropein suppresses oxidative, inflammatory, and apoptotic responses following glycerol-induced acute kidney injury in rats
(Elsevier Inc., 2019) Yin M.; Jiang N.; Guo L.; Ni Z.; Al-Brakati A.Y.; Othman M.S.; Abdel Moneim A.E.; Kassab R.B.; Department of Nephrology; China-Japan Union Hospital of Jilin University; Changchun; Jilin 130033; China; Department of Human Anatomy; College of Medicine; Taif University; Taif; Saudi Arabia; B.Sc. Department; Preparatory Year College; University of Hail; Hail; Saudi Arabia; Faculty of Biotechnology; October University for Modern Science and Arts (MSA); Giza; Egypt; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo; 11795; Egypt
Aim: Here, we evaluated the possible protective effects of oleuropein, the major phenolic constituent in virgin olive oil against glycerol-induced acute kidney injury (AKI) in rats. Main methods: Twenty-eight Sprague Dawley rats were allocated equally into four groups as follows: control group, oleuropein group (50 mg/kg body weight), AKI group and the oleuropein + AKI group. AKI was induced by injecting 50% glycerol (10 ml/kg body weight) intramuscularly. Key findings: Glycerol injection increased the kidney relative weight as well as rhabdomyolysis (RM)- and AKI-related index levels, including the levels of creatine kinase, lactate dehydrogenase, creatinine, urea, and Kim-1 expression. Additionally, alteration in oxidative conditions in renal tissue was recorded, as confirmed by the elevated malondialdehyde and nitric oxide levels and the decreased glutathione content. Concomitantly, the protein and mRNA expression levels of antioxidant enzymes were suppressed. Moreover, Nfe2l2 and Hmox1 mRNA expression was also downregulated. Glycerol triggered inflammatory reactions in renal tissue, as evidenced by the increased pro-inflammatory cytokines and Ccl2 protein and mRNA expression, whereas myeloperoxidase activity was increased. Furthermore, glycerol injection enhanced apoptotic events in renal tissue by increasing the expression of the pro-apoptotic proteins and decreasing that of anti-apoptotic. However, oleuropein administration reversed the molecular, biochemical, and histological alterations resulting from glycerol injection. Significance: Our data suggest that oleuropein has potential as an alternative therapy to prevent or minimize RM incidence and subsequent development of AKI, possibly due to its potent anti-stress, anti-inflammatory, and anti-apoptotic effects. � 2019
Olive (Olea europaea) leaf methanolic extract prevents HCl/ethanol-induced gastritis in rats by attenuating inflammation and augmenting antioxidant enzyme activities
(Elsevier Masson SAS, 2017) Al-Quraishy S.; Othman M.S.; Dkhil M.A.; Abdel Moneim A.E.; Department of Zoology; College of Science; King Saud University; Riyadh; Saudi Arabia; Faculty of Preparatory Year; University of Hail; Hail; Saudi Arabia; Faculty of Biotechnology; October University for Modern Science and Arts (MSA); Giza; Egypt; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo; Egypt
Gastritis is preponderantly characterized by inflammation of the lining epithelial layer and the chronic gastritis is considered as a pre-cancer lesion. For many centuries olive (Olea europaea) leaf has been used for its putative health potential, nonetheless, to date, the gastroprotective effects of olive leaves have not been studied yet. Hence, in this study we investigated whether olive leaf extract (OLE) could protect gastric mucosa against HCl/ethanol-induced gastric mucosal damage in rats. Hcl/ethanol administration caused significant damage to the gastric mucosa, as confirmed by gastric ulcer index and histological evaluation. However, this damage was largely prevented by pre-administering 20�mg/kg omeprazole or 100�mg/kg OLE. Interestingly, the damage was completely prevented by pre-administering 200 and 300�mg/kg OLE. Moreover, OLE attenuated the inflammatory response by decreasing nuclear factor-?B (NF-?B), cycloxygenase-2 (COX-2) and tumor necrosis factor-? (TNF-?) expressions, and down-regulating inducible nitric oxide synthase (iNOS) and interleukin-1? (IL-1?) in gastric mucosa. The gastroprotective mechanism of OLE involved the promotion of enzymatic and nonenzymatic molecules (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione reduced form), promoting nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA expression, halting lipid peroxidation and preventing the overproduction of nitric oxide. Together, our findings clearly demonstrated that OLE could prevent HCl/ethanol-induced gastritis by attenuating inflammation and oxidant/antioxidant imbalance. Indeed, OLE could potentially be useful as a natural therapy for gastritis. � 2017 Elsevier Masson SAS
Oxidative stress and apoptosis are markers in renal toxicity following Egyptian cobra (Naja haje) envenomation
(University of Punjab (new Campus), 2014) Dkhil M.A.; Al-Quraishy S.; Farrag A.R.H.; Aref A.M.; Othman M.S.; Moneim A.E.A.; Department of Zoology; College of Science; King Saud University; Riyadh; Saudi Arabia; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo; Egypt; Pathology Department; Medical Research Division; National Research Centre; Cairo; Egypt; Biological Science Department; Faculty of Dentistry; Modern Sciences and Arts University; Giza; Egypt; Biochemistry and Molecular Biology Department; Faculty of Biotechnology; Modern Science and Arts; Giza; Egypt
Snakebite is a serious and important problem in tropical and subtropical countries including Egypt. The venom of Egyptian cobra (Naja haje; L.) is complex, and it has been considered as a good source of short neurotoxins and several cytotoxins. In this study, oxidative stress inductions as well as apoptotic effects of the Egyptian cobra crude venom at a dose of 0.025mg/kg (intraperitoneal injection; i.p.) has been investigated in kidney of rats after 4 h. Twelve rats divided into 2 groups, Group I served as control group, Group II received i.p. injection of 0.025mg/kg of crude venom. The venom enhanced lipid peroxidation and nitric oxide productions in the kidney with concomitant reduction in glutathione content and superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase activities were inhibited. Moreover, the venom induced a renal injury as indicated by histopathological changes in the kidney tissue with an elevation in serum creatinine and urea. In addition, the renal ultrastructural changes were in the form of blebbing of visceral epithelial cells, and foot process disorganization. Also, the glomerular capillaries lined by hypertrophied endothelial cells. These findings were associated with the pro-apoptotic action in the kidney. The results suggest that Egyptian cobra venom stimulates oxidative stress to induce apoptosis in renal tissue through inhibition of mitochondrial respiration in male rats. Copyright 2014 Zoological Society of Pakistan.
Pomegranate peel attenuates aluminum-induced hepatorenal toxicity
(2013) Abdel Moneim A.E.; Othman M.S.; Mohmoud S.M.; El-Deib K.M.; Department of Zoology and Entomology; Faculty of Science; Helwan University; 11795 Helwan; Cairo; Egypt; Biochemistry and Molecular Biology Department; Faculty of Pharmacy; October University for Modern Science and Arts (MSA); Giza; Egypt; Department of Zoology; Faculty of Science; Cairo University; Cairo; Egypt; Molecular Drug Evaluation Department; National Organization for Drug Control and Research (NODCAR); Giza; Egypt
The present study was undertaken to determine the potential role of methanolic extract of pomegranate peel (MEPP) in modulating aluminum chloride (AlCl3) induced hepatorenal toxicity in female rats. The effect of MEPP (200mg/kgbwt) on AlCl3 (34mg/kgbwt) induced hepatorenal toxicity, accumulation of aluminum (Al), hepatorenal functions and oxidant/antioxidant status of liver and kidney were determined. The changes of liver and kidney structures were investigated with hematoxyline and eosin, in addition, the anti-apoptotic effect of MEPP was analyzed by immunohistochemistry. The present study showed an indication of carcinogenicity in the AlCl3 treated group represented by an increase in tumor necrosis factor-? and angiogenin and inflammation by inducing an increase in prostaglandin E2 and F2?. MEPP protected liver and kidney through reduce the Al accumulation, stimulated antioxidant activities and elevated the anti-apoptotic protein namely Bcl-2. Therefore, these results indicated that the methanolic extract of pomegranate peel has beneficial influences and could be able to inhibit Al-induced oxidative stress and histopathological alternations in liver and kidney of female rats, and these effects may be related to anti-apoptotic and antioxidant activities. � 2013 Informa Healthcare USA, Inc.
The potential effect of berberine in mercury-induced hepatorenal toxicity in albino rats
(Elsevier Ltd, 2014) Othman M.S.; Safwat G.; Aboulkhair M.; Abdel Moneim A.E.; Faculty of Biotechnology; October University for Modern Science and Arts (MSA); Giza; Egypt; Zoology and Entomology Department; Faculty of Science; Helwan University; Cairo; Egypt; Biochemistry and Molecular Biology Department; Asturias Institute of Biotechnology; University of Oviedo; Oviedo; Spain
Mercury (Hg) is the third most dangerous heavy metal after arsenic and lead. Mercury's toxicity brings serious risks to health through negative pathological and biochemical effects. The study was designed to investigate the possible protective role of berberine (BN) in mercuric chloride (HgCl2) induced oxidative stress in hepatic and renal tissues. Adult male albino Wistar rats were exposed to mercuric chloride (HgCl2; 0.4mg/kg bwt) for 7days. Treatment with HgCl2 induced oxidative stress by increasing lipid peroxidation and nitric oxide production along with a concomitant decrease in glutathione and various antioxidant enzymes, namely superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. HgCl2 intoxication increased the activities of liver enzymes and the bilirubin level, in addition to the levels of urea and creatinine in serum. BN (100mg/kg bwt) treatment inhibited lipid peroxidation and nitric oxide production, whereas it increased glutathione content. Activities of antioxidants enzymes, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, were also restored concomitantly when compared to control after BN administration. BN also inhibited the apoptotic effect of HgCl2 by increasing the expression of Bcl-2 protein in liver and kidney. Histopathological examination of the liver and kidney tissues proved the protective effect of BN against HgCl2 toxicity. These results demonstrated that BN augments antioxidant defense against HgCl2-induced toxicity and provides evidence that it has therapeutic potential as hepato- and reno-protective agent. � 2014 Elsevier Ltd.
The potential protective effect of physalis peruviana L. against carbon tetrachloride-induced hepatotoxicity in rats is mediated by suppression of oxidative stress and downregulation of MMP-9 expression
(Elsevier Ltd., 2014) Al-Olayan E.M.; El-Khadragy M.F.; Aref A.M.; Othman M.S.; Kassab R.B.; Abdel Moneim A.E.; Zoology Department; Faculty of Science; King Saud University; Riyadh 11451; Saudi Arabia; Zoology and Entomology Department; Faculty of Science; Helwan University; Cairo 11795; Egypt; Biological Science Department; Faculty of Dentistry; Modern Sciences and Arts (MSA) University; Giza 12111; Egypt; Biochemistry and Molecular Biology Department; Faculty of Biotechnology; Modern Sciences and Arts (MSA) University; Giza 12111; Egypt; Experimental Biology Department; Faculty of Science; Masaryk University; 62500 Brno; Czech Republic; Biochemistry and Molecular Biology Department; Asturias Institute of Biotechnology; University of Oviedo; 33006 Oviedo; Spain
The active constituent profile in Cape gooseberry (Physalis peruviana L.) juice was determined by GC-MS. Quercetin and kaempferol were active components in the juice. In this study we have evaluated its potential protective effect on hepatic injury and fibrosis induced by carbon tetrachloride (CCl4). Twenty-eight rats divided into 4 groups: Group I served as control group, and Group II received weekly i.p. injection of 2 mL CCl4/kg bwt for 12 weeks. Group III were supplemented with Physalis juice via the drinking water. The animals of Group IV received Physalis juice as Group III and also were intraperitoneally injected weekly with 2 mL CCl4/kg bwt for 12 weeks. Hepatoprotective effect was evaluated by improvement in liver enzymes serum levels, reduction in collagen areas, downregulation in expression of the fibrotic marker MMP-9, reduction in the peroxidative marker malonaldehyde and the inflammatory marker nitric oxide, and restoration of the activity of antioxidant enzymatic and nonenzymatic systems, namely, glutathione content, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, and glutathione reductase activities. The results show that the potential hepatoprotective effects of Physalis peruviana may be due to physalis acts by promotion of processes that restore hepatolobular architecture and through the inhibition of oxidative stress pathway. � 2014 Ebtisam M. Al-Olayan et al.
The potential protective role of Physalis peruviana L. fruit in cadmium-induced hepatotoxicity and nephrotoxicity
(Elsevier Ltd, 2014) Dkhil M.A.; Al-Quraishy S.; Diab M.M.S.; Othman M.S.; Aref A.M.; Moneim A.E.A.; Department of Zoology; College of Science; King Saud University; Riyadh; Saudi Arabia; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo; Egypt; Department of Molecular Drug Evaluation; National Organization for Drug Control and Research (NODCAR); Giza; Egypt; Department of Biochemistry and Molecular Biology; Faculty of Biotechnology; Modern Sciences and Arts (MSA); Giza; Egypt; Department of Biological Science; Faculty of Dentistry; Modern Sciences and Arts University (MSA); Giza; Egypt; Department of Biochemistry and Molecular Biology; Asturias Institute of Biotechnology; University of Oviedo; Oviedo; Spain
This study aimed to investigate the potential protective role of Physalis peruviana L. (family Solanaceae) against cadmium-induced hepatorenal toxicity in Wistar rats. Herein, cadmium chloride (CdCl2) (6.5mg/kg bwt/day) was intraperitoneally injected for 5 days, and methanolic extract of physalis (MEPh) was pre-administered to a group of Cd-treated rats by an oral administration at a daily dose of 200mg/kg bwt for 5 days. The findings revealed that CdCl2 injection induced significant decreases in kidney weight and kidney index. Cadmium intoxication increased the activities of liver enzymes and the bilirubin level, in addition to the levels of uric acid, urea and creatinine were increased in the serum. The pre-administration of MEPh alleviated hepatorenal toxicity in Cd-treated rats. Physalis was noted to play a good hepatorenal protective role, reducing lipid peroxidation, nitric oxide, and enhancing enzymatic activities and non-enzymatic antioxidant molecule, glutathione, in hepatic and renal tissues of Cd-treated rats. Moreover, physalis treatment was able to reverse the histopathological changes in liver and kidney tissues and also increased the expression of Bcl-2 protein in liver and kidney of rats. Overall, the results showed that MEPh can induce antioxidant and anti-apoptotic effects and also exerts beneficial effects for the treatment of Cd-induced hepatorenal toxicity. � 2014 Elsevier Ltd.
The potential role of Azadirachta indica treatment on cisplatin-induced hepatotoxicity and oxidative stress in female rats
(2013) Dkhil M.A.; Al-Quraishy S.; Aref A.M.; Othman M.S.; El-Deib K.M.; Abdel Moneim A.E.; Department of Zoology; College of Science; King Saud University; Riyadh 11451; Saudi Arabia; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo 11795; Egypt; Biological Science Department; Faculty of Dentistry; Modern Science and Arts University (MSA); Giza 12111; Egypt; Biochemistry and Molecular Biology Department; Faculty of Pharmacy; Modern Science and Arts (MSA); Giza 12111; Egypt; Molecular Drug Evaluation Department; National Organization for Drug Control and Research (NODCAR); Giza 12553; Egypt; Department of Biochemistry and Molecular Biology; Asturias Institute of Biotechnology; University of Oviedo; Oviedo 33006; Spain
Azadirachta indica A. Juss. (neem, family: Meliaceae) is perhaps the most commonly used traditional medicinal plant of India. In this study we investigated the protective effect of methanolic neem leaves extract (MNLE; 500 mg/Kg bwt) on rats treated with cisplatin (CDDP)-induced hepatotoxicity. Adult rats were randomly divided into four groups. CDDP was given to rats by intraperitoneal injection, while MNLE was given by oral gavage for 5 days after the CDDP injection. The injury and oxidative stress caused by CDDP on the liver and the effect of MNLE were evaluated by measuring (a) histological changes, (b) tissue biochemical oxidant and antioxidant parameters, and (c) investigating apoptosis markers immunohistochemically and by real time PCR. After treatment with MNLE, the histological damage and apoptosis induction caused by cisplatin were improved. Malondialdehyde and nitric oxide were significantly decreased; the antioxidant system, namely, glutathione content, glutathione-S-transferase, glutathione peroxidase, catalase, and superoxide dismutase activities were significantly elevated. In conclusion, MNLE may have a potential role when combined with cisplatin in chemotherapy to alleviate cisplatin-induced damage and oxidative stress in liver. 2013 Mohamed A. Dkhil et al.
The Potential Role of Zinc Oxide Nanoparticles in MicroRNAs Dysregulation in STZ-Induced Type 2 Diabetes in Rats
(Humana Press Inc., 2019) Othman M.S.; Hafez M.M.; Abdel Moneim A.E.; B.Sc. Department; Preparatory Year College; University of Ha�il; Hail; Saudi Arabia; Faculty of Biotechnology; MSA University; Giza; Egypt; Biochemistry Department; Faculty of Pharmacy; Ahram Canadian University (ACU); Giza; Egypt; Zoology and Entomology Department; Faculty of Science; Helwan University; Cairo; Egypt
Diabetes mellitus (DM) is a group of metabolic disorders that are characterized by a loss of glucose homeostasis and insufficiency in production or action of insulin. Development of newly antidiabetic molecules using a variety of organic compounds and biomolecules has been in practice for a long time. Recently, nanomaterials are also being used in antidiabetic studies for their unique properties. In this context, zinc nanoparticles have drawn attention due to the relationship between diabetes and imbalance of zinc homeostasis. Few studies have attempted to investigate the effect of zinc oxide nanoparticles (ZON) in microRNA dysregulations in diabetes. To evaluate the therapeutic effect of ZON on streptozotocin (STZ)-induced diabetic rats as well as its role in microRNA dysregulations. Diabetes was induced in rats by 60�mg/kg body weight (bwt) of STZ and then treated with ZON (5�mg/kg bwt) for 15 consecutive days. The levels of glucose, insulin, oxidative stress markers, and microRNAs expression were measured in liver and pancreas tissues. Intraperitoneal injection of 60�mg/kg bwt of STZ to Wistar rats caused significant decreases in the body weight and Zn contents of pancreas, liver, and kidney. Also, STZ injection increased the blood glucose level and oxidative stress (lipid peroxidation (LPO) and nitric oxide (NO). Meanwhile, STZ decreased blood insulin and pancreatic anti-oxidants. STZ also resulted in ? cell dysfunction and destruction and altered the expression of certain pancreatic and liver microRNAs. ZON treatment for 15�days, at a dose of 5�mg/kg bwt resulted in marked improvements in the blood insulin, glucose tolerance, and structure and function of the pancreatic ? cells. Furthermore, ZON administration reduced LPO and NO, and increased the levels of enzymatic and non-enzymatic anti-oxidants in STZ-induced diabetic rats. It was found also that ZON specifically regulated the expression of pancreatic and liver microRNAs that involved in diabetes development. The obtained results revealed that ZON is a promising antidiabetic agent. The antidiabetic effect of ZON was partially mediated by restoring the oxidants/antioxidants balance and by modulating the alerted microRNAs. � 2019, Springer Science+Business Media, LLC, part of Springer Nature.