MSA Repository "MSAR"

MSAR University's Digital Repository is a documentation and digitization of all university outcomes that are of effective value in the scientific and academic community and reflects the university's image, work, and effective contribution to society Through MSAR Digital Repository, the university managed to collect, store, archive and publish digital content - including documents, audio files, images and data sets - all in a safe place. MSAR is one of the strongest University Digital Repositories in Egypt and documented in the DSPACE community with its latest versions.

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Metabolite profiling of Cucurbita pepo L. in relation to its potential to combat experimental trichinosis
(Elsevier B.V., 2025-04-06) Nashwa R. Mohameda; Seham S. El-Hawary; Fatema R. Saber; Ibrahim E. Sallam; Shaimaa H. El-Sayed
This study aimed to evaluate Cucurbita pepo L. (C. pepo) seed extract against Trichinella spiralis (T. spiralis) through in vivo study for both intestinal and muscular phases. Additionally, an extensive metabolic profiling, for all parts of the fruit of Cucurbita pepo L. (seed, peel and flesh), was adopted using UPLC/ESI-qTOF-MS to reveal the phytoconstituents responsible for the biological activities. Regarding the anthelmintic evaluation of the extracts of C. pepo, the count of T. spiralis adult worm was significantly reduced in the group of mice treated early with C. pepo seed extract (CPSE) and albendazole (ABZ) as compared to the control infected non-treated group. Similarly, the total larval count was significantly reduced in groups treated with CPSE and ABZ with more reduction in the groups that received the treatment in the early phase of infection. Upon staining intestinal sections of the infected mice with haematoxylin and eosin, the analysis revealed the improvement of the inflammatory manifestation induced by the parasite upon treatment with CPSE in both the intestinal and muscular phases. UPLC/ESI-qTOF-MS technique enabled the identification and comprehensive profiling of 79 secondary metabolites, which belonged to various classes of compounds including flavonoids, phenolics, cucurbitacins and fatty acids, in addition to other minor classes. The current study suggests C. pepo seed extract as a promising candidate for management of the gastrointestinal parasites being enriched with bioactive phytoconstituents. © 2025 SAAB. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
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Providencia pseudovermicola sp. nov.: redefining Providencia vermicola and unveiling multidrug-resistant strains from diabetic foot ulcers in Egypt
(BioMed Central Ltd, 2025-04-23) Samira M. Hamed; Manal M. Darwish; Reham Monir; Ahmed Al Taweel; Ayat I. Ghanem; Ihab N. Hanna; Mai A. Amer
Background Providencia species are concerning due to their intrinsic resistance to colistin and tigecycline, complicating the treatment of multidrug-resistant (MDR) infections. Methods In the current study, two MDR isolates, DFU6 and DFU52T , were recovered from infected diabetic foot ulcers in Egypt in 2024. Following their initial identification as Providencia stuartii using VITEK® 2 and MALDI-TOF-MS, the isolates were subjected to whole-genome sequencing via DNBseq. Results While the 16S rRNA gene showed 100% similarity to that of Providencia vermicola, phylogenomic analysis against the type strains in the TYGS database, including P. vermicola DSM 17385T confirmed that these isolates represent a distinct species within the genus, further supported by overall genome-relatedness indices (ORGIs). This discrepancy prompted us to revise the taxonomy of all published genomes of P. vermicola strains (n=59) which revealed misidentification of at least 56 strains that are unrelated to the type strain of this species. DFU6 and DFU52T carried novel sequence types (ST29 and ST41, submitted to PubMLST) and harbored multiple resistance genes. Both strains contained the qnrD1 gene on a small, non-mobilizable plasmid. DFU52T possessed a conjugative plasmid encoding blaCMY−6, blaNDM−1, rmtC, aac(6’)-Ib10, sul1, aph(3’)-Ia, and qacEΔ1. DFU6 carried an ISEcp1-associated blaCTX−M−14, along with aadA, dfrA1, lnuF in a class 2 integron, and armA, msrE, and mphE on a resistance plasmid. Both isolates also featured a pathogenicity island (PAI) integrated into the pheV gene with fimbriae-encoding genes. Conclusion Following our reassessment of the taxonomic classification of all P. vermicola strains with published genomes, we propose reclassifying certain strains, including DFU6 and DFU52T , into distinct species for which we propose the name Providencia pseudovermicola sp. nov. We recommend DFU52T (=CCASU-2024-72) as the type strain for the novel species. We also shed light on the public health threat of this novel species as a human pathogen that harbours carbapenem and aminoglycoside resistance genes on mobile genetic elements.
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Methanol/Kerosene Blends as Fuel
(Elsevier, 2025-03-17) Sadia Abdullaha; Mamoona Munira; Mushtaq Ahmadb; Ahmad Mustafa
Methanol/kerosene blends are the mixtures of methanol and kerosene. These blends have gained tremendous attention now a days as potential substitutes to traditional jet fuels owing to eco-friendly nature, higher flash points and octane number, thus reducing the toxic emissions compared to fossil fuels. China provides methanol fuel at prices that are usually 30%–50% less expensive than petrol and it is currently the largest methanol producer and consumer globally. Automobiles can be modified to operate on increased methanol blends, varying from 500 to 1000 (US$73–146) thereby making methanol the common and accessible fuel for automobiles. Methanol-Kerosene in different blends exhibits promising results when used in aircraft and micro turbo engine. By offering a direct path to drastically cut emissions from industry, shipping, overland transportation, aviation and power generation, methanol produced from renewable source has emerged as a greener and best substitute to traditional fuels. Methanol-kerosene blends can be hazardous and poisonous if not handled properly and safely, the same is also true for petrol and diesel. To tackle the toxic nature of these blends, a multidisciplinary strategy involving chemistry, materials science, toxicology, and environmental science, along with cooperation between academics, stakeholders and regulatory bodies is likely required. Additionally, in depth analysis of methanol-kerosene blends and their generation from biomass and waste products is currently the need of time so as to achieve the sustainability decarbonation and resilience society. © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
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Cinnamic acid Attenuates Cisplatin-Induced Hepatotoxicity and Nephrotoxicity
(National Academy of science, 2016-01-06) Amany A. Tohamy; Ahmed M. Aref; Ahmed E. Abdel Moneim; Romissaa H. Sayed
We investigated the effects of cinnamic acid (CA, 20 mg/kg body weight) on cisplatin (CP)-induced hepto and nephrotoxicity in mice. CP (5 mg/kg bwt) was injected intraperitoneally and CA was given by gastric gavage for 5 days pre- and post-CP injection. After 5 days of CP injection, CP-induced injuries of the hepatic and renal tissues which were evidenced (i) histopathological damage of the hepatic and renal tissues, (ii) as increases in liver and kidney function parameters, (iii) as increases in lipid peroxidation and nitric oxide, and (iv) as decrease in glutathione content. In contrast, the oral administration of CA concurrently to CP intoxicated mice brought back lipid peroxidation, nitric oxide, glutathione levels to near normalcy. Moreover, the histological observations evidenced that CA effectively rescues the liver and kidney from CP mediated oxidative damage. Therefore, cinnamic acid can be considered a potential candidate for protection of hepato-and nephrotoxicity induced by cisplatin.
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Therapeutic Effect of Melatonin and/or Adipose-Derived Mesenchymal Stem Cells Against Doxorubicin-Induced Renal Toxicity in Wister Rats
(Institute of Advanced Studies, 2024-07-22) Romisaa Hussein Sayed; Emad M. El Zayat; Sherein S. Abdelgayed3; Jehane I. Eid; Mohamed Hosney
Doxorubicin (DOX) is highly effective against cancer, but its use is limited due to significant toxicity, particularly in the liver and kidneys. This toxicity is primarily caused by oxidative stress, which results in biochemical, histological, and genetic abnormalities, and disrupts the body's antioxidant defenses. Adipose-derived mesenchymal stem cells (ADMSCs) show promise in cell therapy applications against DOX toxicity Similarly, melatonin supplementation has demonstrated therapeutic potential in mitigating DOX-induced liver and kidney damage by reducing oxidative stress. Understanding and harnessing these therapeutic effects could lead to improved treatments for DOXinduced organ damage. Aims: This study focused on evaluating how melatonin and adipose-derived mesenchymal stem cells (ADMSCs) could mitigate renal toxicity induced by doxorubicin (DOX), a potent anthracycline chemotherapeutic drug known for its severe side effects. The research aimed to explore whether the antioxidant properties of melatonin and the regenerative capabilities of ADMSCs could synergistically alleviate the adverse effects of DOX on renal function in Wistar rats. Methods: The study included two main groups: a control group receiving saline and a DOX group receiving six doses of doxorubicin to induce renal toxicity. Subsequently, rats from both groups were assigned to receive treatments of saline, ADMSCs, melatonin, or a combination of ADMSCs and melatonin. Rats were euthanized at different time points post-treatment, and blood and kidney tissue samples were collected. The analysis included assessment of oxidative stress biomarkers, DNA damage, gene expression profiles, histopathological changes, and cell homing in the kidney tissues. Results: DOX induces oxidative stress, inflammation, and genotoxicity in kidneys while reducing antioxidant enzyme activity. Melatonin and/or ADMSC treatments effectively mitigate these effects by decreasing oxidative stress, inflammation, and DNA damage. The protective mechanisms involve modulation of apoptosis pathways, evidenced by changes in p53 and Bcl2 expression levels, as well as assessment of double-strand breaks using the neutral comet assay, and also the improvement of the inflammatory markers IL-6 & IL- 10 evidenced by the histopathological results. Conclusion: Melatonin and ADMSCs demonstrate protective effects against DOX-induced renal toxicity, potentially enhancing chemotherapy safety by reducing renal damage. The synergistic benefits of combining melatonin and ADMSC therapy in improving chemotherapy tolerability warrant further investigation in preclinical models to optimize treatment strategies and validate efficacy before clinical application.