Evaluation of genexpert MTB/RIF assay for direct diagnosis of pulmonary tuberculosis

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorMoussa H.S.
dc.contributor.authorBayoumi F.S.
dc.contributor.authorAli A.M.
dc.contributor.otherBotany Department
dc.contributor.otherFaculty of Women for Art
dc.contributor.otherScience
dc.contributor.otherand Education
dc.contributor.otherAin Shams University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Microbiology Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherModern Sciences and Arts University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Medical Laboratory Technology
dc.contributor.otherCollege of Applied Medical Science
dc.contributor.otherUniversity of Dammam
dc.contributor.otherHafr Al Batin
dc.contributor.otherSaudi Arabia
dc.date.accessioned2020-01-09T20:41:43Z
dc.date.available2020-01-09T20:41:43Z
dc.date.issued2016
dc.descriptionScopus
dc.description.abstractObjectives: To evaluate the performance of GeneXpert MTB/RIF assay for direct diagnosis of pulmonary tuberculosis (PTB). Methods: This is a cross-sectional study conducted between October 2013 and February 2016 at Abbassaia Chest Hospital and Ain Shams University Hospital, Cairo, Egypt. Inclusion criteria were adults between 18 and 60 years with suspected PTB and classified into 5 clinical categories based on their clinical, radiological, and laboratory findings: confirmed TB, probable TB, possible TB, unlikely TB, and not TB. Two sputum samples from each participant were analyzed by GX and the results were compared by conventional culture. Results: In total, 218 participants were enrolled: 71 had confirmed TB; 112, highly probable TB; 20, probable TB; 10, unlikely TB; and 5, no TB. The sensitivity and specificity of the GX assay were 93% and 98.3% respectively. GeneXpert was positive in 93% of confirmed TB and 2.2% of probable TB cases. Conclusions: GeneXpert is a rapid and promising technique with good sensitivity (93%) and specificity (98.3%), but it cannot be used as a standalone PTB diagnostic tool. There is a need for more GX evaluation studies in countries with low TB incidence. � 2016, Saudi Arabian Armed Forces Hospital. All rights reserved.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=18826&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.15537/smj.2016.10.14998
dc.identifier.doiPubMed ID 27652357
dc.identifier.issn3795284
dc.identifier.otherhttps://doi.org/10.15537/smj.2016.10.14998
dc.identifier.otherPubMed ID 27652357
dc.identifier.urihttps://t.ly/0Ew13
dc.language.isoEnglishen_US
dc.publisherSaudi Arabian Armed Forces Hospitalen_US
dc.relation.ispartofseriesSaudi Medical Journal
dc.relation.ispartofseries37
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectUniversity for Modern Sciences and Arts
dc.subjectMSA University
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectisoniaziden_US
dc.subjectrifampicinen_US
dc.subjectantibiotic sensitivityen_US
dc.subjectArticleen_US
dc.subjectassayen_US
dc.subjectcross-sectional studyen_US
dc.subjectdiagnostic test accuracy studyen_US
dc.subjectgold standarden_US
dc.subjecthumanen_US
dc.subjectlung tuberculosisen_US
dc.subjectmajor clinical studyen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectMycobacterium tuberculosis resistance to rifampicin assayen_US
dc.subjectpredictive valueen_US
dc.subjectreceiver operating characteristicen_US
dc.subjectsensitivity and specificityen_US
dc.subjectadolescenten_US
dc.subjectadulten_US
dc.subjectfemaleen_US
dc.subjectgeneticsen_US
dc.subjectmaleen_US
dc.subjectmicrobiologyen_US
dc.subjectmiddle ageden_US
dc.subjectsputumen_US
dc.subjectTuberculosis, Pulmonaryen_US
dc.subjectyoung adulten_US
dc.subjectAdolescenten_US
dc.subjectAdulten_US
dc.subjectCross-Sectional Studiesen_US
dc.subjectFemaleen_US
dc.subjectHumansen_US
dc.subjectMaleen_US
dc.subjectMiddle Ageden_US
dc.subjectSputumen_US
dc.subjectTuberculosis, Pulmonaryen_US
dc.subjectYoung Adulten_US
dc.titleEvaluation of genexpert MTB/RIF assay for direct diagnosis of pulmonary tuberculosisen_US
dc.typeArticleen_US
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