A tandem green–red heterodimeric fluorescent protein with high FRET efficiency

dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorAlsayed Alaraby Abdelsalam, Mohamad
dc.contributor.authorD Wiens, Matthew
dc.contributor.authorShen, Yi
dc.contributor.authorLi, Xi
dc.contributor.authorSmisdom, Nick
dc.contributor.authorZhang, Wei
dc.contributor.authorBrown, Alex
dc.contributor.authorE Campbell, Robert
dc.date.accessioned2019-10-27T09:33:50Z
dc.date.available2019-10-27T09:33:50Z
dc.date.issued2016
dc.description.abstractThe tetrameric red fluorescent protein from Discosoma sp. coral (DsRed) has previously been engineered to produce dimeric and monomeric fluorescent variants with excitation and emission profiles that span the visible spectrum. The brightest of the effectively monomeric DsRed variants is tdTomato—a tandem fusion of a dimeric DsRed variant. Here we describe the engineering of brighter red (RRvT), green (GGvT), and green–red heterodimeric (GRvT) tdTomato variants. GRvT exhibited 99 % intramolecular FRET efficiency, resulting in long Stokes shift red fluorescence. These new variants could prove useful for multicolor live‐cell imaging applications.en_US
dc.description.sponsorshipWiley-VCHVerlag GmbH&Co. KGaA,Weinheimen_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=16944&tip=sid&clean=0
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dc.identifier.doihttps://doi.org/10.1002/cbic.201600492
dc.identifier.otherhttps://doi.org/10.1002/cbic.201600492
dc.identifier.urihttps://t.ly/jYUe
dc.language.isoenen_US
dc.relation.ispartofseriesChemBioChem;17 , 24 , 2361-2367
dc.subjectHeterodimeric Fluorescenten_US
dc.titleA tandem green–red heterodimeric fluorescent protein with high FRET efficiencyen_US
dc.typeArticleen_US

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