Radiosynthesis, molecular modeling studies and biological evaluation of 99mTc-Ifosfamide complex as a novel probe for solid tumor imaging

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorMotaleb M.A.
dc.contributor.authorEl-Safoury D.M.
dc.contributor.authorAbd-Alla W.H.
dc.contributor.authorAwad G.A.S.
dc.contributor.authorSakr T.M.
dc.contributor.otherLabelled Compounds Department
dc.contributor.otherHot Labs Center
dc.contributor.otherAtomic Energy Authority
dc.contributor.otherCairo
dc.contributor.otherEgypt; Pharmaceutical Chemistry Department
dc.contributor.otherCollege of Pharmaceutical Sciences and Drug Manufacturing
dc.contributor.otherMisr University for Science & Technology
dc.contributor.otherGiza
dc.contributor.otherEgypt; Pharmaceutics and Industrial Pharmacy Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAin Shams University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Radioactive Isotopes and Generator Department
dc.contributor.otherHot Labs Center
dc.contributor.otherAtomic Energy Authority
dc.contributor.otherCairo
dc.contributor.otherEgypt; Pharmaceutical Chemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University of Modern Sciences and Arts (MSA)
dc.contributor.otherGiza
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:47Z
dc.date.available2020-01-09T20:40:47Z
dc.date.issued2018
dc.descriptionScopus
dc.descriptionMSA Google Scholar
dc.description.abstractPurpose: Ifosfamide as a chemotherapeutic drug is used for the treatment of different cancer types. The purpose of this study is the preparation of 99mTc-ifosfamide complex to be evaluated as a potential candidate for tumor imaging. Materials and methods: The radiolabeling of ifosfamide with technetium-99m was carried out by mixing 4mg ifosfamide and 5 ?g of SnCl2.2H2O with 400 MBq Na99mTcO4 at pH 9 for 30 min at room temperature. Computer simulation studies were performed using Accelrys Discovery Studio 2.5 operating system to illustrate the interaction of ifosfamide and 99mTc-ifosfamide complexes with DNA. The in-vivo biodistribution of 99mTc-ifosfamide was studied in tumor-bearing Albino mice. Results: A new 99mTc-ifosfamide complex was synthesized with a good radiochemical yield of 90.3. 2.1% under the optimized conditions and exhibited in-vitro stability up to 2 h. Biodistribution studies showed good uptake in tumor site and high uptake in tumor site with T/NT ?3 after 60 min post-injection. Besides, the molecular docking study confirmed that the complexation of ifosfamide with technetium-99m does not abolish its binding to the target receptor. Conclusion: These promising results afford a new radiopharmaceutical that could be used as a potential tumor imaging. 2018, Copyright 2018 Taylor & Francis Group, LLC.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=29904&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1080/09553002.2019.1524945
dc.identifier.doiPubMed ID 30373490
dc.identifier.issn9553002
dc.identifier.otherhttps://doi.org/10.1080/09553002.2019.1524945
dc.identifier.otherPubMed ID 30373490
dc.identifier.urihttps://t.ly/nv3Mn
dc.language.isoEnglishen_US
dc.publisherTaylor and Francis Ltden_US
dc.relation.ispartofseriesInternational Journal of Radiation Biology
dc.relation.ispartofseries94
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectUniversity of Modern Sciences and Arts
dc.subjectMSA University
dc.subjectIfosfamideen_US
dc.subjectimagingen_US
dc.subjectlabelingen_US
dc.subjecttechnetium-99men_US
dc.subjecttumor targetingen_US
dc.subjectifosfamideen_US
dc.subjectifosfamide complex tc 99men_US
dc.subjectradiopharmaceutical agenten_US
dc.subjecttechnetium 99men_US
dc.subjectunclassified drugen_US
dc.subjectDNA topoisomeraseen_US
dc.subjectifosfamideen_US
dc.subjecttechnetiumen_US
dc.subjectanimal experimenten_US
dc.subjectanimal modelen_US
dc.subjectanimal tissueen_US
dc.subjectArticleen_US
dc.subjectcomplex formationen_US
dc.subjectcomputer simulationen_US
dc.subjectcontrolled studyen_US
dc.subjectdrug distributionen_US
dc.subjectdrug DNA interactionen_US
dc.subjectdrug screeningen_US
dc.subjectdrug stabilityen_US
dc.subjectdrug synthesisen_US
dc.subjectfemaleen_US
dc.subjectin vitro studyen_US
dc.subjectin vivo studyen_US
dc.subjectisotope labelingen_US
dc.subjectmolecular dockingen_US
dc.subjectmolecular imagingen_US
dc.subjectmolecular modelen_US
dc.subjectmouseen_US
dc.subjectnonhumanen_US
dc.subjectpriority journalen_US
dc.subjectradiochemistryen_US
dc.subjectsolid malignant neoplasmen_US
dc.subjectanimalen_US
dc.subjectchemistryen_US
dc.subjecthumanen_US
dc.subjectkineticsen_US
dc.subjectmetabolismen_US
dc.subjectmolecular dockingen_US
dc.subjectmolecular imagingen_US
dc.subjectproceduresen_US
dc.subjectprotein domainen_US
dc.subjectsynthesisen_US
dc.subjecttissue distributionen_US
dc.subjectAnimalsen_US
dc.subjectDNA Topoisomerases, Type Ien_US
dc.subjectDrug Stabilityen_US
dc.subjectHumansen_US
dc.subjectIfosfamideen_US
dc.subjectKineticsen_US
dc.subjectMiceen_US
dc.subjectMolecular Docking Simulationen_US
dc.subjectMolecular Imagingen_US
dc.subjectProtein Domainsen_US
dc.subjectRadiochemistryen_US
dc.subjectTechnetiumen_US
dc.subjectTissue Distributionen_US
dc.titleRadiosynthesis, molecular modeling studies and biological evaluation of 99mTc-Ifosfamide complex as a novel probe for solid tumor imagingen_US
dc.typeArticleen_US
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