Three smart spectrophotometric methods for resolution of severely overlapped binary mixture of Ibuprofen and Paracetamol in pharmaceutical dosage form

Thumbnail Image

Date

2019

Journal Title

Journal ISSN

Volume Title

Type

Article

Publisher

Springer International Publishing

Series Info

BMC chemistry;13, 1, 2019

Abstract

Paracetamol is an analgesic-antipyretic drug and Ibuprofen is a non-steroidal anti-inflammatory drug. They are co-formulated as tablets to improve analgesia, to simplify prescribing and to improve patient compliance. Three accurate, simple and sensitive spectrophotometric methods were developed for the simultaneous determination of Paracetamol and Ibuprofen in their co-formulated dosage form. The first method was the ratio difference, which was based on the measurement of the difference in absorbance between the two wavelengths (210.6 and 216.4 nm) for Ibuprofen and (236.0 and 248.0 nm) for Paracetamol. The second method was constant center method which depends on using the constant found in the ratio spectra. The third method was the mean centering of ratio spectra which measured the manipulated values at 240 nm and 237 nm for Ibuprofen and Paracetamol, respectively. Beer’s law was obeyed in the concentration range of 2–50 μg/mL for Ibuprofen and 2–20 μg/mL for Paracetamol. The recovery % of the accuracy of both methods ranged from 99.64 to 100.56%. Factors affecting the resolution of the spectra were studied and optimized. The three methods are validated according to ICH guidelines and could be applied for the pharmaceutical preparation.

Description

MSA Google Scholar

Keywords

University of Ibuprofen, Paracetamol, Ratio Difference

Citation

1. Brunton L, Parcxzcke K, Blumenthal D, Buxton L (2008) Goodman &Gilman’s: manual of pharmacology and therapeutics. McGraw-Hill Inc., New York Google Scholar 2. Katzung BG, Trevor AJ (2009) Basic & clinical pharmacology. McGraw-Hill, New York Google Scholar 3. Joshi RS, Pawar NS, Katiyar SS, Zope DB, Shinde AT (2011) Development and validation of UV spectrophotometric methods for simultaneous estimation of Paracetamol and Ibuprofen in pure and tablet dosage form. Der Pharmacia Sinica. 2(3):164–171 Google Scholar 4. Hoang VD, Ly DTH, Tho NH, Minh Thi Nguyen H (2014) UV spectrophotometric simultaneous determination of Paracetamol and Ibuprofen in combined tablets by derivative and wavelet transforms. Sci World J 2014:1–13 Google Scholar 5. Issa YM, Zayed SIM, Habib IHI (2011) Simultaneous determination of ibuprofen and paracetamol using derivatives of the ratio spectra method. Arab J Chem. 4(3):259–263 CrossRefGoogle Scholar 6. Hassan WS (2008) Determination of Ibuprofen and Paracetamol in binary mixture using chemometric-assisted spectrophotometric methods. Am J Appl Sci. 5(8):1005–1012 CrossRefGoogle Scholar 7. Mallah MA, Sherazi STH, Mahesar SA, Khaskheli AR (2012) Simultaneous quantification of Ibuprofen and Paracetamol in tablet formulations using transmission Fourier transform infrared spectroscopy. Am J Anal Chem. 3(8):503–511 CrossRefGoogle Scholar 8. Pinho JSA, Luna AS (2014) Determination of Paracetamol and Ibuprofen in tablets and urine using spectrofluorimetric determination coupled with chemometric tools. Austin J Anal Pharm Chem. 1(1):1001–1007 Google Scholar 9. Battu PR, Reddy M (2009) RP-HPLC method for simultaneous estimation of Paracetamol and Ibuprofen in tablets. Asian J Chem 2(1):70–72 Google Scholar 10. Jahan MS, Islam MJ, Begum R, Kayesh R, Rahman A (2014) A study of method development, validation, and forced degradation for simultaneous quantification of Paracetamol and Ibuprofen in pharmaceutical dosage form by RP-HPLC method. Anal Chem Insights 9:75–81 PubMedPubMedCentralGoogle Scholar 11. Raja MG, Geetha G, Sangaranarayanan A (2012) Simultaneous, stability indicating method development and validation for related compounds of Ibuprofen and Paracetamol tablets by RP-HPLC method. J Chromatogr Sep Tech. 3(8):3–8 CrossRefGoogle Scholar 12. Mumtaz A, Rizwana MI (2015) Simultaneous estimation of Paracetamol, Ibuprofen and Famotidine by using RP-HPLC method. Indo Am J Pharm Res 5(9):2964–2981 Google Scholar 13. Lakka NS, Goswami N, Balakrishna P (2011) Development and validation of a RP-HPLC for simultaneous determination of Ibuprofen and Paracetamol in solid dosage forms: application to dissolution studies. Int J Res Pharm Sci 2(3):331–337 Google Scholar 14. Lotfy HM, Salem H, Abdelkawy M, Samir A (2015) Spectrophotometric methods for simultaneous determination of Betamethasone valerate and Fusidic acid in their binary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 140:294–304 CrossRefGoogle Scholar 15. Zaazaa HE, Elzanfaly ES, Soudi AT, Salem MY (2015) Application of the ratio difference spectrophotometry to the determination of ibuprofen and famotidine in their combined dosage form: comparison with previously published spectrophotometric methods. Spectrochim Acta A Mol Biomol Spectrosc. 143:251–255 CrossRefGoogle Scholar 16. Youssef SH, Hegazy MA-M, Mohamed D, Badawey AM (2018) Analysis of paracetamol, pseudoephedrine and cetirizine in Allercet Cold((R)) capsules using spectrophotometric techniques. Chem Cent J 12(1):67–81 CrossRefGoogle Scholar 17. Mohamed EH, Lotfy HM, Hegazy MA, Mowaka S (2017) Different applications of isosbestic points, normalized spectra and dual wavelength as powerful tools for resolution of multicomponent mixtures with severely overlapping spectra. Chem Cent J 11(1):43–58 CrossRefGoogle Scholar 18. El-Maraghy CM, Mohamed EH (2018) Successive stability indicating spectrophotometric technique for simultaneous determination of Quetiapine Fumarate and its three major related compounds. Curr Anal Chem 14:1–9 CrossRefGoogle Scholar 19. Lotfy HM (2012) Determination of simvastatin and ezatimibe in combined tablet dosage forms by constant center spectrophotometric method. Int J Pharm Pharm Sci. 4:673–679 Google Scholar 20. Lotfy H, Mowaka S, Mohamed E (2016) Validated spectrophotometric methods for simultaneous determination of omeprazole, tinidazole and doxycycline in their ternary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 153:321–332 CrossRefGoogle Scholar 21. Afkhami A, Bahram M (2005) Mean centering of ratio spectra as a new spectrophotometric method for the analysis of binary and ternary mixtures. Talanta 66(3):712–720 CrossRefGoogle Scholar 22. ICH (2005) International Conference on Harmonization, of Technical Requirements for registration of Pharmaceuticals for Human Use Topic Q2 (R1). Validation of analytical procedures: text and methodology. Geneva Google Scholar

Full Text link