Expression, detection of candidate function and homology modeling for Vicia villosa ornithine δ-aminotransferase

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorM.K. Nada, Ahmed
dc.contributor.authorM. Abd-Elhalim, Haytham
dc.contributor.authorM. El-Domyati, Fotouh
dc.contributor.authorM. I. Abou-Ali, Rania
dc.contributor.authorBahieldin, Ahmed
dc.date.accessioned2020-02-02T10:40:56Z
dc.date.available2020-02-02T10:40:56Z
dc.date.issued2010
dc.descriptionMSA Google Scholaren_US
dc.description.abstractThe accumulation of compatible solutes during stress in plant cell is well documented. Proline is one of these solutes that accumulates in the cytosol in response to drought or salinity stress in plants. Proline has several functions during stress just like osmotic adjustment, osmoprotection, free radical scavenger and antioxidant. Ornithine δ-aminotransferase (δ-OAT) is an important enzyme in proline biosynthetic pathway. It catalyzes the transamination of ornithine to pyrroline5-carboxylate, which can be reduced into proline. Expression of ornithine δ-aminotransferase gene isolated from Vicia villosa (VvOAT) showed protein with a molecular mass of 63 KDa, which is compatible with the predicted mass and after VvOAT gene delivery into E. coli host HB101, VvOAT gene enhanced its salt tolerance. Homology modeling of VvOAT was performed based on the crystal structure of the ornithine δ-aminotransferase from humans (PDB code 2OATA). With this model, a flexible docking study with the substrate and inhibitors was performed. The results indicated that PHE170 and ASN171 in VvOAT are the important determinant residues in binding as they have strong hydrogen bonding contacts with the substrate and inhibitors. All the obtained results indicated the efficiency of utilizing this gene in conferring salt toleranceen_US
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dc.identifier.issn1938-1999 (Print)
dc.identifier.issn1938-2006 (Online)
dc.identifier.urihttps://cutt.ly/rtyvNCL
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.relation.ispartofseriesGM crops;1:4, 250-256
dc.subjectprolineen_US
dc.subjectδ-aminotransferaseen_US
dc.subjectSDS-PAGEen_US
dc.subjectbacterial transformationen_US
dc.subjecthomology modelingen_US
dc.subjectsalt toleranceen_US
dc.titleExpression, detection of candidate function and homology modeling for Vicia villosa ornithine δ-aminotransferaseen_US
dc.typeArticleen_US

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