Serratia marcescens P25, A New Strain Isolated From The Phycoplane of the Red Marine Alga Punctaria sp Produced Potent Biosurfactant Used for Enhancing the Bioremediation of Spent Motor Oil-Polluted Soil
| dc.Affiliation | October University for modern sciences and Arts (MSA) | |
| dc.contributor.author | Diab, Ali | |
| dc.contributor.author | Ageez, Amr | |
| dc.contributor.author | Gardoh, Iman | |
| dc.date.accessioned | 2019-10-19T10:24:30Z | |
| dc.date.available | 2019-10-19T10:24:30Z | |
| dc.date.issued | 2015 | |
| dc.description | MSA Google Scholar | |
| dc.description.abstract | Samples of marine algae were collected from a coastal area at Abokir, Alexandria, and identified as: Punactraia sp (brown alga), Colpomenia sp (brown alga), Jania sp (red alga) and Ulva sp (green alga). 25 bacterial strains were isolated and purified from the phycoplane of Punctaria sp and were screened for the production of biosurfactants using cheap substrate (waste frying oil). The production of biosurfactants was tested by the plug agar and the ODA method. 60% and 80% of the tested strains were biosurfactant producers when the plug agar and the ODA method respectively used. Five red pigmented strains were very active biosurfactant producers (158-167.6 Cm2 ODA) were selected and studied for their emulsification activity and their stability at wide range of temperature (0-121°C), pH values (2-12) and salinity (5-25% NaCl-W/V). The selected five bacterial strains were identified as members of Serratia marcescens. Strain P25 was characterized by producing a biosurfactant of more stability at wide range of temperature, pH and salinity, this is in addition to its ability to produce high emulsion activity against spent motor oil. The produced emulsion was stable at 7-30 days. The above characters give this Serratia marcescens P25 strain a potential application in petroleum industry such as cleaning oil storage tanks, recovery of oil from oily sludge, microbial enhanced oil recovery (MEOR),washing oil- contaminated soil and enhancing the bioremediation of hydrocarbon- contaminated sites. The cell free culture broth (supernatant) containing the biosurfactant that was produced by strain P25 was sterilized and applied for the bioremediation of spent motor oil- contaminated soil. The result show that the addition of the sterilized supernatant alone increased the biodegradation of the oil to 65.0±5.2%. Addition of NP fertilizer alone failed to increase the biodegradation more than 46.0±2.0%, while in the presence of a mixture of biosurfactant and NP (BRNP) the biodegradation increased to 60.0±5.0%. Statistically, no significant difference between the result in presence of BR in the presence of BRNP (P>0.05). It can be concluded that the promising factor in the biodegradation of spent motor oil is the addition of BR alone or in combination with NP. | en_US |
| dc.description.sponsorship | International Journal of Science and Research | en_US |
| dc.identifier.citation | [1] Abdou,A.M.2003. Purification and partial characterization of psychrotrophic Serratia marcescens lipase.J.Dairy Sci.86:127-132. [2] Aboulseoud, M., Maachi,R.,Amrane,A.,Boundergua,S.,Nabi,A.2008.E valuation of different carbon and nitrogen sources in production of biosurfactant by Pseudomonas flourescens.Desalination. 223:143-151. [3] Ahmed, E.F., Hassan, S.S. 2013.Antimicrobial activity of a bioemulsifier produced by Serratia marcescens SIO.J.AL-Nahrain Univ. 16(1):147-155. [4] Akoachere, T.K., Jane-Francis, A., Theresa, N., Felicitas, Y.N., Nkwelang, G., Ndip, R.N.2008.Lubricating oil-degrading bacteria in soils from filling stations and aut-mechanic workshops in Buea, Afr. J. Biotechnol.7:1700-1706. [5] Al-Bahry, S.N., Al-Wahaibi, Y.M., Elshafie, A.E., Al- Bemani, A.S., Joshi, S.S., Al.-Makhmaie, H.S, Al- Sulaimani, H.S. 2012. Biosurfactant production by Bacillus subtilis B2O using date molasses and its possible application in enhanced oil recovery. Int Biodeter. Biodegrad xxx:1.6. [6] Amin, S.A., Hemelo, L.R., Vantop, H.M, Durham, B.P., Carison, L.T., Heal, k.R. 2015. Interaction and signaling between a cosmopolitan phytoplankton and associated bacteria. Nature, 522:98-101. [7] Anyanwu, C.W., Obi, S.K.,C. Okola, B.N. 2011. Lipopeptide biosurfactant production by Serratia marcescens NSK-1 strain isolated from petroleum contaminated soil. J. Appl. Sci. Res.7(1):79-87. [8] Atuanya, E.J. 1987. Effect of oil pollution on physical and chemical properties of soil: a case study of waste oil contaminated delta soil in Bandel state, Nigeia J. Appl. Sci.55:155-176. [9] Bell, W., Mitchell, R. 1972. Chemotactic and growth responses of marine bacteria to algal extracellular products. Biol. Bull. 143:265-277. [10] Cameotra, S.S., Singh, P. 2008. Bioremediation of oil sludge using crude biosurfactants. Int. Biodeter. Biodegrad. 62:274-280. [11] Chen, C.S., Rao, P.S.C., Lee, L.S. 1996. Evaluation of extraction methods for determining polynuclear aromatic hydrocarbons from contaminated soil. Chemosphere. 32:1123:1132. [12] Dhail, S., Jsuj, N.D. 2012. Isolation of biosurfactantproducing marine bacteria. Afr. J. Environ. Sci. Technol. 6(6):263-266. [13] Diab, A. and El Din, S.G. 2013. Production and characterization of biosurfactants produced by Bacillus spp and Pseudomonas spp isolated from rhizosphere soil of an Egyptian salt marsh plant. Nature and Science, 11(5):103-112 [14] Diab, A., Metwalli, A. 1982. Bacillus species associated with Phaeophyceae in the Arabian Gulf shore water at Kuwait. Zbl. Microbiol. 137:197-206. [15] Dusane, D.H., Pawar, V.S., Nancharaiah, Y.V., Venugopalan, V.P., Kumar, A.R., Zinjarde, S.S. 2011. Antibiofilm potential of a glycolipid surfactant produced by a tropical marine strain of Serratia marcescens. Biofouling, 27(6):645-654. [16] El Sanoussi, S.M., El Sarag, M.S.A., Mohamed, S.E. 1987. Properties of Serratia marcescens isolated from diseased honeybee (Apis mellifera) Larvae. J. General Microbiol. 133:215-219. [17] Elemba, O.M., Ijah, U.J.J., Chibunna, M. 2015. Isolation, characterization and MEOR ability of the biosurfactant produced from Serratia Marcescens UEO 15. Global J.Advanced Res.2(6): 962-974. [18] Eruke, O.S. and Udoh, A.J. 2015. Potential for biosurfactant enhanced bioremediation of hydrocarboncontaminated soil and water. A Review. Advances in Research. 4(1):1-4. [19] Everson, K.L., Bromel, M.C., Anderson, A.W., Freeman, T.P. 1984. Bacterial symbionts in the sugar beet root maggot, Tetanops myopaeformis (von Roder). Applied and Environmental Microbiology, 47:22-27. [20] Gracia- Hernandez, D., Sosa-Aguirre, C.R., Sanchez-Yanez, J.M. 2001. Bioremediation of domestic sewage polluted with waste motor oil. Hydraulic Engineering in Mexico, 22:113-118. [21] Grimont, F., Grimont, P.A.D. 2006. The Genus Serratia. Prokaryotes. 6:219-244. [22] Grimont, P.A.D., Grimont, F. Starr, M.P. 1981. Serratia species isolated from plants. Current Microbiology, 5:317-322. [23] Gudina, E.S., Teixeira, J.A., Rodrigues, L.R. 2016. Biosurfactant production by marine microorganisms with therapeutic applications. Marine Drugs 2016, 38; dio:10.3390/md14020038. [24] Gundhimathi, R., Kiran, G.S., Hema, T.A., Selvin, J., Ravii, T.R., Shanmughapriya, S. 2009. Production and characterization of lipopeptide biosurfactant by a sponge- associated marine actinomycetes Nocardiopsis alba MSA10. Bioprocess Biosyst. Eng. 32: 825-835. [25] Haddad, N.I.A., Wang, Ji, Bazhong, Mu. 2009. Identification of a biosurfactant producing strain of Bacillus subtilis HOB2. Protein and Peptide letters. 16:7-13. [26] Halt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T. 1994. Bergey’s Manual of Determinative Bacteriology, 9th Ed. Williams and Wilkins, Baltimore. [27] Hamza, A., Sabturani, N., Radiman, S. 2013. Screening and optimization of a biosurfactant production by the hydrocarbon degrading bacteria. Sains Malaysiana, 42(5):615-623. [28] Helmy, Q, Kardina, E., Nurachmen, Z., Wisjnuprapto. 2010. Application of biosurfactant produced by Azotobacter vinelandii for enhanced oil recovery and biodegradation of oil sludge. Int. J. Civil. Environ. Engin ISCEE. 10(1):7-14. [29] Helvia, W., de Araujo, V., Fukushima, K., Campos- Takaki, G.M. 2010. Prodigiosin production of Serratia marcesens UCP 1549 using renewable resources as low cost substrate. Molecules. 15:6931-6940. [30] Hunachimso, E.O., Josiah, I.U. 2015. Isolation, characterization and MEOR ability of the biosurfactant produced by Serratia marcescens UE015. Global J. Adv. Res.2 (6):962-974. [31] Jain, P.K., Gupta,V.K., Gaur, R.K., Lowry, M., Jaroli, D.P., Chauhan, U.K. 2009. Bioremediation of petroleum oil contaminated soil and water. Research J. Environ. Toxicology, 5(1):1-26. [32] Kang, S.w., Kin, Y.B., Shin, J.D., Kim, E.X. 2010. Enhanced biodegradation of hydrocarbons in soil by microbial biosurfactant, sophorolipid. App. Biochem. Biotechnol. 160:780-790. [33] Khopade, A., Biao, R., Liu, X., Mahad, k.k., Zhang, L., Kakara, C. 2012. Production characterization and stability studies of biosurfactant isolated from marine Nocardiopsis sp B4. Desalination. 285:198-204. [34] Kim, B-H., Ramanan, R., Cho, D-H., oh, H-H., Kim, H-S. 2014. Role of Rhizobium, a plant growth promoting bacterium, in enhancing algal biomass through mutualistic interaction. Biomass Bioenergy. 69:95-105. [35] Kiran, G.S., Hema, T.A., Gandhimathi, R., Selvin, J., Thomas, T.A., Ravji, T.R., Natarajaseenivasan, K., 2009. Optimization and production of a biosurfactant from the sponge- associated marine fungus Aspergillus ustus MSF3. Colloids Surf. B. Biointerfaces. 73:250- 256. [36] Kiran, G.S., Sabarathnam, B., Selvin, J, 2010 a. Biofilm disruption potential of a glycolipid biosurfactant from marine Brevibacterium casei. FEMS Immunol. Med. Microbiol. 59:432-438. [37] Kiran, G.S., Thajuddin, N., Hema, T.A., Idhayadhulla, A., Kumar, R.S. Selviun, J. 2010 b. Optimization and characterization of rhamnolipid biosurfactant from sponge associated marine fungi Aspergillus Sp MSF1. Desalination and Water Treatment. 24:257-265. [38] Kiran, G.S., Thomas, T.A., Selvin, J., Sabarathnam, B., Lipton, A.2010 c. Optimization and characterization of a new lipopeptide biosurfactant produced by marine Brevibacterium aureum MSA 13 in soild state culture. Bioresouces Technol. 101:2389-2396. [39] Lawniczak, L., Marecik, R., Chizanowski, L. 2013. Contribution of biosurfactant to natural or induced bioremediation. Appl. Microbiol. Biotechnol. 97:2327- 2339. [40] Lima, T.M.S., Fonseco, A.F., Leao, B.A., Hoounteer, A.H., Totola, M.R., Borges. A.C. 2011. Oil recovery from fuel oil storage tank sludges using biosurfactant. J. Biorem. Biodegrad. 2:125-137. [41] Lloyed, C.A., Cackette, T.A. 2001. Diesel engines: Environmental impact and control. Air and Waste Management Association. 51:825-842. [42] Mabrouk, M.E.O., Youssif, E.M., Sabry, S.A. 2014. Biosurfactant production by a newly isolated soft coralassociated marine Bacillus sp E24. Statistical optimization and characterization. Life Sci. J. 11(10):756-763. [43] Mandri, T., Lin, J. 2007. Isolation and characterization of engine oil-degrading indigenous microorganisms in Kwazulu Natal. South Africa. Afric. J. Biotechnal. 6:23- 27. [44] Mishra, S.J., Jyot, R.C., Kuchad, B.L. 2001. Evaluation of inoculum addition to stimulate insitu bioremediation of oil sludge contaminated soil. Appl. Environ. Microbiol. 67(4):1675-1681. [45] Morikawa, M., Mirata, Y., Imanaka, T. 2000. A study on the structure relationship of the lipopeptide biosurfactant. Biochem. Biophys. Acta. 1488:211-218. [46] Mulligan, C.N., Gibbs, B.F.2004. Types production and application of biosurfactant. Proc, Sci.Acad.1:31- 55. [47] Munachimso, E.O., Josiah, I.U.J., Miracle, C.2015. Isolation characterization and MEOR ability of the biosurfactant produced by Serratia marcescens UEO 15. Global j. Advanced Res.2(6):962-974. [48] Neto, D.M., Meira, J.A., de Araujo, J.M., Mitchel, D.A., Krieger, N. 2008.Optimization of the production of rhamnolipids by Pseudomonas aeruginasa UFPEDA614 in solid state culture. Appl. MicrobiPl. Biotechnol. 81:441-448. [49] Objegba, V.J., Sadiq, A.O. 2002. Effect of spent engine oil on the growth parameters, chlorophyll, and protein levels of Amaranthus hybridus L. The Environment. 22:23-28. [50] Peter, J.K., Rao, A.K., Kumari, R. 2014. Analysis of individual and bacterium consortium of broth culture of Pseudomonas aeruginosa, serratia marcescens and Bacillus subtilis to emulsify oils and hydrocarbons. Int. J. Res. 1(7):769-783. [51] Plaza, G.A., Zjawiony, I., Banat, I.M. 2006. Use the different methods for detection of thermophilic biosurfactant- producing bacteria from hydrocarboncontaminated and bioremediated soils. J. Petrol. Sci. Eng. 50:71-77. [52] Plociniczak, M.P., Plaza, J.A., Seget, Z.P., Cameotra, S.S. 2011. Environmental application of biosurfactants: Recent Advances. Int. J. Mol. Sci. 12:633-654. [53] Ramanan. R., Kim, B-H., Cho, D-H., Oh, H.M., Kim, H.S. 2016.Evaluation, ecology and emerging application. Biotechnol. Advances. 34:14-29. Brevibacterium aureum MSA 13 in soild state culture. Bioresouces Technol. 101:2389-2396. [39] Lawniczak, L., Marecik, R., Chizanowski, L. 2013. Contribution of biosurfactant to natural or induced bioremediation. Appl. Microbiol. Biotechnol. 97:2327- 2339. [40] Lima, T.M.S., Fonseco, A.F., Leao, B.A., Hoounteer, A.H., Totola, M.R., Borges. A.C. 2011. Oil recovery from fuel oil storage tank sludges using biosurfactant. J. Biorem. Biodegrad. 2:125-137. [41] Lloyed, C.A., Cackette, T.A. 2001. Diesel engines: Environmental impact and control. Air and Waste Management Association. 51:825-842. [42] Mabrouk, M.E.O., Youssif, E.M., Sabry, S.A. 2014. Biosurfactant production by a newly isolated soft coralassociated marine Bacillus sp E24. Statistical optimization and characterization. Life Sci. J. 11(10):756-763. [43] Mandri, T., Lin, J. 2007. Isolation and characterization of engine oil-degrading indigenous microorganisms in Kwazulu Natal. South Africa. Afric. J. Biotechnal. 6:23- 27. [44] Mishra, S.J., Jyot, R.C., Kuchad, B.L. 2001. Evaluation of inoculum addition to stimulate insitu bioremediation of oil sludge contaminated soil. Appl. Environ. Microbiol. 67(4):1675-1681. [45] Morikawa, M., Mirata, Y., Imanaka, T. 2000. A study on the structure relationship of the lipopeptide biosurfactant. Biochem. Biophys. Acta. 1488:211-218. [46] Mulligan, C.N., Gibbs, B.F.2004. Types production and application of biosurfactant. Proc, Sci.Acad.1:31- 55. [47] Munachimso, E.O., Josiah, I.U.J., Miracle, C.2015. Isolation characterization and MEOR ability of the biosurfactant produced by Serratia marcescens UEO 15. Global j. Advanced Res.2(6):962-974. [48] Neto, D.M., Meira, J.A., de Araujo, J.M., Mitchel, D.A., Krieger, N. 2008.Optimization of the production of rhamnolipids by Pseudomonas aeruginasa UFPEDA614 in solid state culture. Appl. MicrobiPl. Biotechnol. 81:441-448. [49] Objegba, V.J., Sadiq, A.O. 2002. Effect of spent engine oil on the growth parameters, chlorophyll, and protein levels of Amaranthus hybridus L. The Environment. 22:23-28. [50] Peter, J.K., Rao, A.K., Kumari, R. 2014. Analysis of individual and bacterium consortium of broth culture of Pseudomonas aeruginosa, serratia marcescens and Bacillus subtilis to emulsify oils and hydrocarbons. Int. J. Res. 1(7):769-783. [51] Plaza, G.A., Zjawiony, I., Banat, I.M. 2006. Use the different methods for detection of thermophilic biosurfactant- producing bacteria from hydrocarboncontaminated and bioremediated soils. J. Petrol. Sci. Eng. 50:71-77. [52] Plociniczak, M.P., Plaza, J.A., Seget, Z.P., Cameotra, S.S. 2011. Environmental application of biosurfactants: Recent Advances. Int. J. Mol. Sci. 12:633-654. [53] Ramanan. R., Kim, B-H., Cho, D-H., Oh, H.M., Kim, H.S. 2016.Evaluation, ecology and emerging application. Biotechnol. Advances. 34:14-29. [54] Sarmento, H., Gazol, J.M.2012. Use of phytoplanktonderived dissolved organic carbon by different types of bacterioplanktons. Environ. Microbiol. 14:2348-2360. [55] Satpute, S.K., Banpurkar, A.G., Dhakephalkar, P.K.M., Banat, I.M., Chopade, B.A. 2010. Methods for investigating biosurfactant and bioemulsifiers: a review. Critical Reviews in Biotechnology. 30(2):124- 144. [56] Scrascia, M., Pazzani, F., Oliva, M., Russo, V., D’Addabbo, P., Porcelli, F. 2016.Identification of pigmented Serratia marcescens symbiotically associated with Rhynchophorus ferrugineus Olivier (Coleoptera Curculionidae). Open ACCESS Original Research. Microbiology open published by John Wiley Sonsltd. [57] Silvin, J., Shanmughapriya, S., Gandhimathi, R. Kiran. G.S., Ravji, T.R. 2009.Optimization and production of novel antimicrobial agents from spongeassociated marine actinomycetes Nocardiopsis dassanvillei MAD08. Apppl. Microbial. Biotechnol. 83:435-445 [58] Tambekar, D.H., Gadakh, P.V. 2013. Biochemical and molecular detection of biosurfactant- producing bacteria from soil. Int. J. Life Sci. and farm Res. 2(11):204-2011. [59] Techaoei, S., Lumyon, S., Prathumpai, W., Santiarwarn, D., Leelapornpisid, P. 2011. Screening, characterization and stability of biosurfactant produced by Pseudomonas aeruginosa SCMV 102 isolates from soil in Northern Thailand. Assian J. Biological Science. 4(4):340-351. [60] Thavasi, R., Jayalakshmi, S., Banat, I.M. 2011. Effect of biosurfactant and fertilizer on biodegradation of crude oil by marine isolates of Bacillus megaterum, Corynebacterium Kutscheri and Pseudomonas aeruginosa. Biores. Technol. 102:772-778. [61] Thavasi, R., Subramanyam- Nambaru, V.R.M., Jayalalshmi, S., Balasubramanian, T., Banat, I.M. 2010. Biosurfactant production by Pseudomonas aeruginosa from renewable sources. Int. J. Microbiol. 5(7):200-450. [62] Ugoh, S.C., Moneke, L.U. 2011. Isolation of bacteria from engine oil contaminated soils in auto mechanic workshops in Gwagwalada Abiya, FCT-Nigeria Academic Arena. 3(5): 28-33. [63] Vazquez- Duhalt, R., Bartha, R. 1989. Biodegradation of petroleum in soil environment. Cand. J. Microbiol. 20:1985-1988. [64] Willumsen, P.A., Karison, U.1997. Screening of bacteria isolated from PAH- contaminated soil for production of biosurfactant and bioemulsifier. Biodegradation. 7:415-423. [65] WU, Y., Luo, y., Zou, D., Ni, J., Liu, w., Teng, Y. 2008. Bioremediation of polycylic aromatic hydrocarbons contaminated soil with Monilinia sp. Degradation and microbial community analysis. Biodegradation 19:247-257. [66] Youssef, N.H., Duncan, K.E., Nagle, D.D., Savage, K.H., Knapp, R.M., Mclnerney, M.J.2004. Comparison of methods to detect biosurfactant production by diverse microorganisms. J. Microbiol. Methods. 56:339-347. | en_US |
| dc.identifier.issn | 2319-7064 | |
| dc.identifier.uri | https://cutt.ly/arAXDiU | |
| dc.language.iso | en | en_US |
| dc.publisher | International Journal of Science and Research | en_US |
| dc.relation.ispartofseries | International Journal of Science and Research (IJSR); | |
| dc.subject | University for Serratia marcescens | en_US |
| dc.subject | Marine algae | en_US |
| dc.subject | Biosurfactant | en_US |
| dc.subject | Bioremediation | en_US |
| dc.subject | Spent motor oil | en_US |
| dc.title | Serratia marcescens P25, A New Strain Isolated From The Phycoplane of the Red Marine Alga Punctaria sp Produced Potent Biosurfactant Used for Enhancing the Bioremediation of Spent Motor Oil-Polluted Soil | en_US |
| dc.type | Article | en_US |