Rapid quantitative detection of Enteric viruses in River Nile and drainage water, Egypt
| dc.Affiliation | October University for modern sciences and Arts (MSA) | |
| dc.contributor.author | Ibrahim Azzam, Mohamed | |
| dc.contributor.author | M. Ezzat, Safaa | |
| dc.contributor.author | A. El-Dougdoug, Khaled | |
| dc.contributor.author | A. Othman, Badawi | |
| dc.date.accessioned | 2019-10-21T08:19:08Z | |
| dc.date.available | 2019-10-21T08:19:08Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Contamination of surface water with enteric viruses is a major public health concern that warrants the need to develop reliable indicators for enteric viruses contamination. This work assesses the bio-diversity of coliphages to use as bio-indicators for viral water pollution. The study elucidates the occurrence of enteric viruses at various locations in Rosetta Branch of the Nile River in addition to five main drains located on its sides which selected for confirmation the eco-diversity of aquatic viral isolates. The evaluation was carried out using real time-quantitative reverse transcriptase - polymerase chain reaction (rt-qRT-PCR). Eight coliphage isolates were detected in both Rosetta Branch and drainage water samples. Transmission Electron microscopy revealed that, the isolated coliphages have an isometric head and long-contractile tail; some particles revealed a short tail with full heads, resembling those belong to the myoviridae and siphoviridae family. Restriction enzymes by EcoRI, HindIII and BamHI showed the presence of double stranded (ds) DNA as well as heterogeneity among these phages. The results showed that EcoRI produced 7, 6, 2, 9, 10, 8, 10, 7 unique fragments and HindIII produced 2, 3, 0, 1, 2, 5, 4, 3 unique fragments while BamHI produced only 4, 0, 1, 1, 0, 4, 2, 1 unique fragments, for the eight phage isolates, respectively. Out of fifteen tested sites, two only (El-Rahawy drain outlet and Sabal drain outlet) were found to be polluted with enteroviruses with rate of 3.6X104 and 3.4X104 gene copies per microliters (GC μl-1), respectively using rt-qRT-PCR. | en_US |
| dc.description.sponsorship | ResearchGate | en_US |
| dc.identifier.citation | Accolas, J.P. and Spillmann, H. (1979). The morphology of six bacteriophages of Streptococcus thermophilus. J. Appl. Bacteriol. 47: 135-144. Adeniji, J.A. and Faleve, T.O. (2014). Isolation and Identification of Enteroviruses from Sewage and Sewage-Contaminated Water in Lagos, Nigeria. Food Environ. Virol. 6(2):75-86. Alla, G.; Eugene, K.; Alina, I.; Anatoly, M. and Andrey, L. (2007). The Diversity of Coliphages and Coliforms in Horse Feces Reveals a Complex Pattern of Ecological Interactions. Appl. Environ. Microbiol. 73(19):5975-5981. Amenu, D. (2014). Isolation of Bacteriophages Specific to Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Bio-Genetic J. 2(1):32-36. American Public Health Association “APHA” (2005). Standard Methods for the Examination of Water and Wastewater (21st ed.), Washington, D. C. Azzam, M.I.; Hakim, A.S.; Othman, B.A. and El-Dougdoug, K.A. (2010). Virological evaluation of drinking water in Egypt. Egyptian J. Virol., SP. Issue, 435-452. Azzam, M.I.; Hakim, A.S.; Othman, B.A. and El-Dougdoug, K.A. (2009). Evaluation of microbial typing as indicators for the efficiency of water treatment stations in El-Menofia Rayah, Egypt. Egyptian J. Virol. 6:435-452. Chandra, M.; Thakur, S.; Narang, D. and Saxena, H. M. (2011). Isolation of a bacteriophage against Salmonella Dublin and determination of its physical resistance under varied in vitro conditions. African Journal of Microbiology Research. 5(15):2044-2047. Chongmiao, Z.; Xiaochang, W.; Yongjum, L. and Dangcong, P. (2010). Simultaneous detection of enteroviruses from surface waters by real-time RT-PCR with universal primers. J. Environ. Sci. 22(8):1261-1266. Cormier, J.; Gutierrez, M.; Goodridge, L. and Janes, M. (2014). Concentration of enteric virus indicator from seawater using granular activated carbon. J. Virol. Methods. 196:212-8. Dakkak, A. (2013). "Egypt's Water Crisis-A Recipe for Disaster". Middle East and North Africa. Available at: www.ecomena.org. [Quoted, 23.10.2013]. Dhevagi, P. and Anusuya, S. (2013). A novel approach for pathogen reduction in wastewater treatment. J. Environ. Health Sci. and Eng. 11:12. Donovan, E.; Unice, K.; Roberts, J.D.; Harris, M. and Finley, B. (2008). Risk of gastrointestinal disease associated with exposure to pathogens in the water of the lower Passaic River. Appl. Environ. Microbiol. 74: 994-1003. Enan, G.; Shaaban, K. A.; Askora, A. and Maher, M. (2013). Coliphages Lysing Escherichia coli Bacteria: Their Morphology and Their Response to Some Physical Factors. Research Journal of Applied Sciences. 8(10):516-522. Espinosa, A.C.; Mazari-Hiriart, M.; Espinosa, R.; Maruri-Avidal, L.; Méndes, E. and Arias, C.F. (2008). Infectivity and genome persistence of rotavirus and astrovirus in groundwater and surface water. Wat. Res. 42: 2618-2628. Hamza, I.A.; Jurzik, L.; Stang, A.; Sure, K.; Überla, K. and Wilhelm, M. (2009). Detection of human viruses in rivers of a densely-populated area in Germany using a virus adsorption elution method optimized for PCR analyses. Wat. Res. 43: 2657-2668. Hamza, I. A; Jurzik, L.; Überla, K. and Wilhelm, M. (2011). Methods to detect infectious human enteric viruses in environmental water samples. International Journal of Hygiene and Environmental Health. 214 (6):424–436. Hegazy, A. M.; El-Salakawy, A. H.; Shaban, M.M.; Yehia, M.M. and Abu Salam, M.S. (2014). Eco-friendly management of enteroviruses in wastewater. Wat. Sci. 27 (54):19-29. Hryniszyn, A.; Skonieczna, M. and Wiszniowski, J. (2013). "Methods for Detection of Viruses in Water and Wastewater," Advances in Microbiology. 3 (5): 442-449. Jebri, S.; Hmaied, F.; Lucena, F.; Saavedra, M.; Yahya, M. and Hamdi, M. (2014). A comparison of two extraction methods for the detection of Enteroviruses in raw sludge. J. Virol. Methods. 200:1-5. Juang, D.F. and Morgan, J.M. (2001). The applicability of the API 20 E and API rapid NET systems for the identification of bacteria from activated sludge. Elect. J. Biotech. 4(1); 0717-3458. Jurzik, L.; Hamzaa, I.A.; Puchert, W.; Überla, K. and Wilhelm, M. (2010). Chemical and microbiological parameters as possible indicators for human enteric viruses in surface water. Int. J. Hyg. Environ. Health. 213:210–216. Jothikumar, N.; Kang, G. and Hill, V.R. (2009). Broadly reactive TaqMan assay for real-time RT-PCR detection of rotavirus in clinical and environmental samples. J. Virol. Methods. 155:126–131. Katzenelson, E.; Fattal, B. and Hostovsky, T. (1976). Organic flocculation: an efficient second-step concentration method for the detection for viruses in tap water. Appl. Environ. Microbial. 59: 1213-1219. Kfir, R.; Hilner, C.; du Preez, M. and Bateman, B. (1995). Studies evaluating the applicability of utilizing the same concentration techniques for the detection of protozoan parasites and viruses in water. Wat. Sci. Techol. 31: 417-423. Lee, S.H. and Sobsey, M.D. (2011). Survival of prototype strains of somatic coliphage families in environmental waters and when exposed to UV low-pressure monochromatic radiation or heat. Wat. Res. 45(12):3723-3734. Lin, J. and Ganesh, A. (2013). Water quality indicators: bacteria, coliphages, enteric viruses. International Journal of Environmental Health Research. 23(6): 484-506. Mans, J.; Netshikweta, R.; Magwalivha, M.; Van Z.W. and taylor M.B. (2013). Diverse norovirus genotypes identified in sewage-polluted river water in South Africa. Epidemiol. Infect. 141 (2): 303-313. Mookerjee, S.; Batabyal, P.; Halder, M. and Palit ,A. (2014). Specificity of coliphages in evaluating marker efficacy: A new insight for water quality indicators. J. Virol. Methods. 208:115-8. Nguyen, X. H.; Fang, T.; Qinqin, B.; Chanjuan, S.; Thanh, T. V. and Chengping, L. (2013). Isolation and characterization of bacteriophages against enterotoxigenic E.coli strains causing swine diseases. African J. of Microbiology Research. 7:1-7. Obire, O.; Lockhart, M. and Putheti, R. (2010). Plaque Assay and Nucleic Acid Analysis of Lytic Coliphages from Creek Water and Domestic Sewage. Academic Journal. 2 (7): 335 Othman, B.A. (1997). Isolation of lambdoid bacteriophage β4 EC from sewage polluted drinking water. Proceedings of the 9th Conference of Microbiology, Cairo, pp: 25-27. Okoh, A. I. and Chigor, V. N. (2012). Quantitative RT-PCR Detection of Hepatitis A Virus, Rotaviruses and Enteroviruses in the Buffalo River and Source Water Dams in the Eastern Cape Province of South Africa. Int. J. Environ. Res. 9(11):4017-4032. Okoh, A. L. and Sibanda, T. (2013). Real-time PCR quantitative assessment of hepatitis A virus, rotaviruses and enteroviruses in the Tyume River located in the Eastern Cape Province, South Africa. Water Sci. 39 (2): 295-304. Oliveira, A.; Sillankorva, S.; Quinta, R.; Henriqoes, A.; Sereno, R. and Azeredo, J. (2008). Isolation and characterization of bacteriophages for avian pathogenic E.coli strains. J. of App. Microbiol. 106:1919-1927. Pettibone, G.W. (1992). The use of Lauryl tryptose broth containing 4-methyl lumbelliferyl-beta-D-glucuronide“MUG” to enumerate E.coli from fresh water sediment. Letters in applied microbiology, 15 (5):190-192. Rutjes, S.A.; Italiaander, R.; Berg, H.L.; Lodder, W.J. and Husman, M.R. (2005). Isolation and detection of enteroviruses RNA from large-volume water samples by using the NucliSens mini MAG system and real-time-RT-PCR nucleic acid sequence-based amplification. National Institute for Public Health and the Environment, American Society for Microbiology. Appl. Environ. Microbiol., 71(7): 3734-3740. Sambrook, J.; Fritschi, E.F. and Maniatis, T. (1989). Molecular cloning: a laboratorymanual, Cold Spring Harbor Laboratory Press, New York. Sinclair, R.S.; Jones E.L. and Gerba, C.P. (2009). Viruses in recreational water-borne disease outbreaks: a review. J. Appl. Microbiol. 107:1769-1780. Śliwa-Dominiak, J.; Tokarz-Deptuła, B. and Deptuła, W. (2014). Occurrence of F-Specific RNA Coliphages and microbial indicators in municipal lake water. Pol. J. Environ. Stud. 23(2):467-473. Tan, E.L.; Yong, L.L.; Quak, S.H; Yeo, W.C. and Chow, V.T. (2008). Rapid detection of enterovirus 71 by real-time TaqMan RT-PCR. J. Clin. Virol. 42: 203–206. Watanabe, K; Takesue, S.; Jin-Nai, K. and Yoshikawa, T. (1970). Bacteriophage Active Against the Lactic acid Beverage. Producing Bacterium Lactobacillus casei. Appl. Microbiol. 20:409-415. Wilkes, G.; Edge, T.; Gannon, V.; Jokinen, C.; Lyautey, E.; Medeirose, D.; Neumann, N.; Ruecker, N.; Topp, E. and Lapen, D.R. (2009). Seasonal relationships among indicator bacteria, pathogenic bacteria, Cryptosporidium oocysts, Giardia cysts, and hydrological indices for surface water within an agricultural landscape. Wat. Res., 43:2209-2223. Xiao, Y.Y.; Xing, M.; Yong, L. Z.; Wen, Q.X.; Xia, N.H.; Yu, G.C. and Kang, D. G. (2012). Real-Time PCR Detection of Enteric Viruses in Source Water and Treated Drinking Water in Wuhan, China. Current Microbiology J. 65 (3):244-253. Yates, M. V. (2007). Classical indicators in the 21st century – far and beyond the coliform. Wat. Environ. Res. 79:279–286. Zahraa, J. J.; Amin, S. B. and Akeel, H. A. (2014). Bacteriolytic Activity of Coliphages on Diarrhea Associated E. coli. Journal of Natural Sciences Research. 4(6):69-75. | en_US |
| dc.identifier.uri | http://central-library.msa.edu.eg:8009/xmlui/handle/123456789/467 | |
| dc.language.iso | en | en_US |
| dc.publisher | ResearchGate | en_US |
| dc.relation.ispartofseries | Egyptian J. Virol;2 | |
| dc.subject | University of Coliphages | en_US |
| dc.subject | Drainage water | en_US |
| dc.title | Rapid quantitative detection of Enteric viruses in River Nile and drainage water, Egypt | en_US |
| dc.type | Article | en_US |