Antifibrotic effects of gallic acid on hepatic stellate cells: In vitro and in vivo mechanistic study

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorEl-Lakkany N.M.
dc.contributor.authorEl-Maadawy W.H.
dc.contributor.authorSeif el-Din S.H.
dc.contributor.authorSaleh S.
dc.contributor.authorSafar M.M.
dc.contributor.authorEzzat, Shahira M
dc.contributor.authorMohamed S.H.
dc.contributor.authorBotros S.S.
dc.contributor.authorDemerdash Z.
dc.contributor.authorHammam O.A.
dc.contributor.otherDepartment of Pharmacology
dc.contributor.otherTheodor Bilharz Research Institute
dc.contributor.otherWarak El-Hadar
dc.contributor.otherImbaba P.O. Box 30
dc.contributor.otherGiza
dc.contributor.other12411
dc.contributor.otherEgypt; Department of Pharmacology and Toxicology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.other11562
dc.contributor.otherEgypt; Department of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.other11562
dc.contributor.otherEgypt; Department of Immunology
dc.contributor.otherTheodor Bilharz Research Institute
dc.contributor.otherWarak El-Hadar
dc.contributor.otherImbaba P.O. Box 30
dc.contributor.otherGiza
dc.contributor.other12411
dc.contributor.otherEgypt; Department of Pathology
dc.contributor.otherTheodor Bilharz Research Institute
dc.contributor.otherWarak El-Hadar
dc.contributor.otherImbaba P.O. Box 30
dc.contributor.otherGiza
dc.contributor.other12411
dc.contributor.otherEgypt; Department of Pharmacology and Biochemistry
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherThe British University in Egypt
dc.contributor.otherSuez Desert Road
dc.contributor.otherP.O. Box 43
dc.contributor.otherElSherouk City
dc.contributor.otherCairo 11837
dc.contributor.otherEgypt; Department of Pharmacognosy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA)
dc.contributor.other6th of October
dc.contributor.otherGiza
dc.contributor.other12566
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:46Z
dc.date.available2020-01-09T20:40:46Z
dc.date.issued2019
dc.descriptionScopus
dc.descriptionMSA Google Scholar
dc.description.abstractFew studies reported the antifibrotic effects of gallic acid (GA) despite its known hepatoprotective and antioxidant activities. Accordingly, this study investigated the antifibrotic effects of GA through clarifying its mechanisms on hepatic stellate cells� (HSCs) activation, proliferation and/or apoptosis. In vitro effects of GA on HSC-T6 activation/proliferation, morphology and safety on hepatocytes were assessed. In vivo, hepatic fibrosis was induced via chronic thioacetamide (TAA)-intoxication. TAA-intoxicated rats were treated with silyamrin or GA. At end of experiment, liver functions, hepatic MDA, GSH, PDGF-BB, TGF-?1, TIMP-1 and hydroxyproline were determined. Histological analysis and Sirius red staining of hepatic sections, expressions of alpha-smooth muscle actin (?-SMA), proliferating cellular nuclear antigen (PCNA) and caspase-3 were examined. In vitro, GA resulted in a concentration and time-dependent inhibition in HSCs activation, proliferation (IC50= 45 and 19 ?g/mL at 24 and 48 h respectively); restored the quiescent morphology of some activated HSCs plus its safety on hepatocytes. In vivo, GA reduced ALT, AST, MDA, PDGF-BB levels, collagen deposition and fibrosis score (S1 vs S4); increased caspase-3 expression and restored GSH stores, TGF-?1 level, ?-SMA and PCNA expressions. In conclusion, GA counteracted the progression of hepatic fibrosis through reduction of HSCs proliferation/activation mutually with their apoptosis induction. � 2018 Center for Food and Biomolecules, National Taiwan Universityen_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=21100287117&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1016/j.jtcme.2018.01.010
dc.identifier.doiPubMed ID :
dc.identifier.issn22254110
dc.identifier.otherhttps://doi.org/10.1016/j.jtcme.2018.01.010
dc.identifier.otherPubMed ID :
dc.identifier.urihttps://t.ly/7OJlB
dc.language.isoEnglishen_US
dc.publisherNational Taiwan Universityen_US
dc.relation.ispartofseriesJournal of Traditional and Complementary Medicine
dc.relation.ispartofseries9
dc.subjectApoptosisen_US
dc.subjectGallic aciden_US
dc.subjectHepatic stellate cellsen_US
dc.subjectHepatocytesen_US
dc.subjectProliferation/activationen_US
dc.titleAntifibrotic effects of gallic acid on hepatic stellate cells: In vitro and in vivo mechanistic studyen_US
dc.typeArticleen_US
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