Genetic variability of somaclonal variation in micropropagated Jatropha curcas L.

Abstract

Jatropha curcas L. has immense economic potential, ecological and environmental significance. This work was conducted to develop a protocol for indirect plant regeneration using mature seeds as in vitro establishment plant material and test the feasibility of using Randomly Amplified Polymorphic DNA (RAPD) for establishing a genetic basis for somaclonal variation produced throughout such protocol. Maximum percentage of germination was recorded on wetted cotton with distilled water when cultures were incubated at 28C. Adding different combinations of growth regulators to the MS (Murashige and Skoog) culture medium could induce callus formation and stimulated callus growth. In this concern, the combination of 2.5 mg L-1 BA + 0.1 mg L-1 IBA was an appropriate supplement compared with other tested treatments. Formed calli were transferred to regeneration MS medium containing different combinations of adenine sulphate (AdS) and cytokinins; BA and Kin. Medium contained 25 mg L-1 AdS combined with 1 mg L-1 BA was propitious to plant regeneration. Data on the effect of different growth regulators on multiplication of regenerated shoots revealed that, the treatments devoid of BA caused callus reinitiation, while the highest concentration of BA in absence of both Kin and IBA produced high shoot number of suitable length with lower callus formation level, which indicates that it is the best shoot multiplication treatment . As per the current study RAPD analysis provided a good tool to detect the genetic diversity in J. curcas.