An eco-friendly direct injection HPLC method for methyldopa determination in serum by mixed-mode chromatography using a single protein-coated column

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorEmara S.
dc.contributor.authorMasujima T.
dc.contributor.authorZarad W.
dc.contributor.authorKamal M.
dc.contributor.authorFouad M.
dc.contributor.authorEl-Bagary R.
dc.contributor.otherPharmaceutical Chemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherMisr International University
dc.contributor.otherKm 28 Ismailia Road
dc.contributor.otherCairo
dc.contributor.otherEgypt; P.I. Lab. Single Cell MS
dc.contributor.otherRIKEN Quantitative Biology Center
dc.contributor.other6-2-3
dc.contributor.otherFuruedai
dc.contributor.otherSuita
dc.contributor.otherOsaka 565-0874
dc.contributor.otherJapan; Pharmaceutical Analytical Chemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherModern Sciences and Arts University
dc.contributor.other26 July Mehwar Road Intersection With Wahat Road
dc.contributor.other6 October City
dc.contributor.otherEgypt; Pharmaceutical Chemistry Department
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherKasr El Aini St.
dc.contributor.otherCairo
dc.contributor.other11562
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:42:01Z
dc.date.available2020-01-09T20:42:01Z
dc.date.issued2015
dc.descriptionScopus
dc.description.abstractA simple, rapid and environment-friendly direct injection HPLC method for the determination of methyldopa (MTD) in human serum has been developed and validated. The method was based on cleanup and separation of MTD from serum by mixed-mode liquid chromatography using a single protein-coated TSK gel ODS-80 TM analytical column (50 x 4.0 mm i.d., 5 ?m). The protein-coated column exhibited excellent resolution, selectivity and functioned in two chromatographic modes: size-exclusion chromatography [i.e., solid-phase extraction (SPE) for serum proteins] and reversed-phase chromatography for the final separation of MTD. SPE and HPLC separation were carried out simultaneously with a green mobile phase consisting of acetate buffer (0.1 M, pH 2.4) at a flow rate of 1 mL/min and at room temperature (23 � 1�C). The eluent was monitored at emission and excitation wavelengths of 320 and 270 nm, respectively. A calibration curve was linear over the range of 0.1-30 ?g/mL with a detection limit of 0.027 ?g/mL. This online SPE method was successfully applied to real samples obtained from patients receiving MTD therapy. � The Author 2015. Published by Oxford University Press. All rights reserved.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=24023&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1093/chromsci/bmv024
dc.identifier.doiPubMed ID 25834172
dc.identifier.issn219665
dc.identifier.otherhttps://doi.org/10.1093/chromsci/bmv024
dc.identifier.otherPubMed ID 25834172
dc.identifier.urihttps://t.ly/NXMvx
dc.language.isoEnglishen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofseriesJournal of Chromatographic Science
dc.relation.ispartofseries53
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectUniversity for Modern Sciences and Arts
dc.subjectMSA University
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectmethyldopaen_US
dc.subjectadulten_US
dc.subjectblooden_US
dc.subjectdevicesen_US
dc.subjecthigh performance liquid chromatographyen_US
dc.subjecthumanen_US
dc.subjectlimit of detectionen_US
dc.subjectmaleen_US
dc.subjectproceduresen_US
dc.subjectreproducibilityen_US
dc.subjectsize exclusion chromatographyen_US
dc.subjectsolid phase extractionen_US
dc.subjectstatistical modelen_US
dc.subjectAdulten_US
dc.subjectChromatography, Gelen_US
dc.subjectChromatography, High Pressure Liquiden_US
dc.subjectHumansen_US
dc.subjectLimit of Detectionen_US
dc.subjectLinear Modelsen_US
dc.subjectMaleen_US
dc.subjectMethyldopaen_US
dc.subjectReproducibility of Resultsen_US
dc.subjectSolid Phase Extractionen_US
dc.titleAn eco-friendly direct injection HPLC method for methyldopa determination in serum by mixed-mode chromatography using a single protein-coated columnen_US
dc.typeArticleen_US
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