Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammation
Date
2024-02
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
King Saud University
Series Info
Saudi Pharmaceutical Journal;32 (2024) 101968
Scientific Journal Rankings
Abstract
Asthma is a chronic disease affecting people of all ages. Asthma medications are associated with adverse effects
restricting their long-term usage, demanding newer alternative therapies. This study aimed to investigate the
anti-asthmatic properties of Ruta graveolens extract and its prepared nano-cubosomal dispersion (Ruta-ND).
Firstly, the R. graveolens methanolic extract exhibited higher anti-inflammatory activity on Lipopolysaccharide
(LPS)-activated BEAS-2B cells. To ensure best bioavailability and hence best cellular uptake, R. graveolens extract
was loaded in nano-cubosomal dispersion (ND). Then, the anti-asthmatic effects of Ruta extract and ND were
simultaneously evaluated in rats’ model with ovalbumin-induced allergic asthma. R. graveolens extract and RutaND subsided asthma score and improved lung function by restoring FEV1/FVC ratio to the expected values in
control rats. Also, it showed strong antioxidant and anti-inflammatory activities manifested by lowering levels of
malondialdehyde (MDA), IL-4, IL-7, TGF-β, and Ig-E, and increasing levels of superoxide dismutase (SOD) and
INF-γ in bronchoalveolar lavage fluid. Our research findings also indicate autophagy induction and apoptosis
inhibition by Ruta extract and Ruta-ND. Finally, the HPLC MS/MS phytochemical profiling of R. graveolens
extract evident production of various alkaloids, flavonoids, coumarins, and other phenolics with reported
pharmacological properties corresponding to/emphasize our study findings. In conclusion, R. graveolens
exhibited promise in managing Ova-induced allergic asthma and could be developed as an alternative antiallergic asthma drug.
Description
Keywords
Apoptosis; Autophagy; Beclin-1; Behavior scoring; Ovalbumin Allergic airway; Respiratory function