Studying the influence of formulation and process variables on Vancomycin-loaded polymeric nanoparticles as potential carrier for enhanced ophthalmic delivery

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorYousry C.
dc.contributor.authorElkheshen S.A.
dc.contributor.authorEl-laithy H.M.
dc.contributor.authorEssam T.
dc.contributor.authorFahmy R.H.
dc.contributor.otherDepartment of Pharmaceutics and Industrial Pharmacy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherKasr El-Aini St
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Pharmaceutics and Pharmaceutical Technology
dc.contributor.otherFaculty of Pharmaceutical Sciences and Pharmaceutical Industries
dc.contributor.otherFuture University in Egypt
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Pharmaceutics and Industrial Pharmacy
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherUniversity of Modern Science and Art
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherKasr El-Aini St
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Pharmaceutics
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAhram Canadian University
dc.contributor.other6th of October City
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:22Z
dc.date.available2020-01-09T20:41:22Z
dc.date.issued2017
dc.descriptionScopus
dc.description.abstractOcular topically applied Vancomycin (VCM) suffers poor bioavailability due to its high molecular weight and hydrophilicity. In the present investigation, VCM-loaded polymeric nanoparticles (PNPs) were developed aiming to enhance its ocular bioavailability through prolonging its release pattern and ophthalmic residence. PNPs were prepared utilizing double emulsion (W/O/O), solvent evaporation technique. 23 � 41 full factorial design was applied to evaluate individual and combined influences of polymer type, Eudragit� RS100, sonication time, and Span�80 concentration on PNPs particle size, encapsulation efficiency, and zeta potential. Further, the optimized formulae were incorporated in 1% Carbopol�-based gel. In-vivo evaluation of the optimized formulae was performed via Draize test followed by microbiological susceptibility testing on albino rabbits. Results revealed successful formulation of VCM-loaded PNPs was achieved with particle sizes reaching 155 nm and up to 88% encapsulation. Draize test confirmed the optimized formulae as non-irritating and safe for ophthalmic administration. Microbiological susceptibility testing confirmed prolonged residence, higher Cmax. with more than two folds increment in the AUC(0.25�24) of VCM-PNPs over control groups. Thus, VCM-loaded PNPs represent promising carriers with superior achievements for enhanced Vancomycin ophthalmic delivery over the traditional use of commercially available VCM parenteral powder after constitution into a solution by the ophthalmologists. � 2017 Elsevier B.V.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=21331&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1016/j.ejps.2017.01.013
dc.identifier.doiPubMed ID 28089661
dc.identifier.issn9280987
dc.identifier.otherhttps://doi.org/10.1016/j.ejps.2017.01.013
dc.identifier.otherPubMed ID 28089661
dc.identifier.urihttps://t.ly/DXrml
dc.language.isoEnglishen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofseriesEuropean Journal of Pharmaceutical Sciences
dc.relation.ispartofseries100
dc.subjectDraize testen_US
dc.subjectMicrobiological susceptibility testingen_US
dc.subjectOcular infectionen_US
dc.subjectPoly (D,L-lactide- coglycolide) (PLGA)en_US
dc.subjectPolycaprolactone (PCL)en_US
dc.subjectVancomycinen_US
dc.subjectcarbomeren_US
dc.subjecteudragiten_US
dc.subjectnanoparticleen_US
dc.subjectpolymeren_US
dc.subjectsolventen_US
dc.subjectsorbitan oleateen_US
dc.subjectvancomycinen_US
dc.subjectacrylic acid resinen_US
dc.subjectantiinfective agenten_US
dc.subjectdrug carrieren_US
dc.subjecteudragit rsen_US
dc.subjectgelen_US
dc.subjectnanoparticleen_US
dc.subjectvancomycinen_US
dc.subjectanimal tissueen_US
dc.subjectarea under the curveen_US
dc.subjectArticleen_US
dc.subjectcontrolled studyen_US
dc.subjectdrug bioavailabilityen_US
dc.subjectdrug delivery systemen_US
dc.subjectdrug formulationen_US
dc.subjectdrug releaseen_US
dc.subjectemulsionen_US
dc.subjectencapsulationen_US
dc.subjectfactorial designen_US
dc.subjectin vivo studyen_US
dc.subjectmaximum plasma concentrationen_US
dc.subjectnonhumanen_US
dc.subjectparticle sizeen_US
dc.subjectpriority journalen_US
dc.subjectultrasounden_US
dc.subjectzeta potentialen_US
dc.subjectanimalen_US
dc.subjectchemistryen_US
dc.subjectdrug effectsen_US
dc.subjectdrug formulationen_US
dc.subjectgelen_US
dc.subjectintraocular drug administrationen_US
dc.subjectpHen_US
dc.subjectrabbits and haresen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectAcrylic Resinsen_US
dc.subjectAdministration, Ophthalmicen_US
dc.subjectAnimalsen_US
dc.subjectAnti-Bacterial Agentsen_US
dc.subjectDrug Carriersen_US
dc.subjectDrug Compoundingen_US
dc.subjectDrug Liberationen_US
dc.subjectGelsen_US
dc.subjectHydrogen-Ion Concentrationen_US
dc.subjectNanoparticlesen_US
dc.subjectRabbitsen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectVancomycinen_US
dc.titleStudying the influence of formulation and process variables on Vancomycin-loaded polymeric nanoparticles as potential carrier for enhanced ophthalmic deliveryen_US
dc.typeArticleen_US
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