Integrating Artifcial Intelligence with Quality by Design in the Formulation of Lecithin/Chitosan Nanoparticles of a Poorly Water‑Soluble Drug
Date
2023-08
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Springer International Publishing AG
Series Info
AAPS PharmSciTech;(2023) 24:169
Scientific Journal Rankings
Abstract
The aim of the current study is to explore the potential of artifcial intelligence (AI) when integrated with Quality by Design
(QbD) approach in the formulation of a poorly water-soluble drug, for its potential use in carcinoma. Silymarin is used as
a model drug for its potential efectiveness in liver cancer. A detailed QbD approach was applied. The efect of the critical
process parameters was studied on each of the particle size, size distribution, and entrapment efciency. Response surface
designs were applied in the screening and optimization of lecithin/chitosan nanoparticles, to obtain an optimized formula.
The release rate was tested, where artifcial neural network models were used to predict the % release of the drug from the
optimized formula at diferent time intervals. The optimized formula was tested for its cytotoxicity. A design space was
established, with an optimized formula having a molar ratio of 18.33:1 lecithin:chitosan and 38.35 mg silymarin. This resulted
in nanoparticles with a size of 161 nm, a polydispersity index of 0.2, and an entrapment efciency of 97%. The optimized
formula showed a zeta potential of +38 mV, with well-developed spherical particles. AI successfully showed high predic-
tion ability of the drug’s release rate. The optimized formula showed an enhancement in the cytotoxic efect of silymarin
with a decreased IC50 compared to standard silymarin. Lecithin/chitosan nanoparticles were successfully formulated, with
deep process and product understanding. Several tools were used as AI which could shift pharmaceutical formulations from
experience-dependent studies to data-driven methodologies in the future.
Description
Keywords
artifcial neural network , deep learning , hepatocellular carcinoma , Ishikawa diagram , lecithin chitosan nanoparticles , quality by design , response surface design