An outbreak of methicillin resistant staphylococcus epidermidis among neonates in a hospital in Saudi Arabia

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorEl Hafez M.A.
dc.contributor.authorKhalaf N.G.
dc.contributor.authorEl Ahmady M.
dc.contributor.authorEl Aziz A.A.
dc.contributor.authorEl Gawad Hashim A.
dc.contributor.otherDepartment of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherHelwan University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherModern Arts and Science University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Medicine
dc.contributor.otherZagazig University
dc.contributor.otherElSharkiya
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherTanta University
dc.contributor.otherTanta
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-25T19:58:29Z
dc.date.available2020-01-25T19:58:29Z
dc.date.issued2011
dc.descriptionScopus
dc.description.abstractIntroduction: Staphylococcus epidermidis is a pathogen associated with nosocomial infection in neonatal intensive care units (NICU). This study investigates an outbreak of methicillin resistant S. epidermidis in an NICU in a hospital in Saudi Arabia. Methodology: A total of 41 isolates identified as Gram-positive cocci were obtained from blood culture, umbilical wound swabs and endotracheal aspirate specimens of neonates, of which 29 were identified as S. epidermidis. Bacterial identification at the species level and determination of antibiotic resistance were performed by MicroScan (Dade Behring, USA). Genotyping was completed using randomly amplified polymorphic DNA (RAPD) and the mecA gene was detected by PCR. Results: All 29 S. epidermidis isolates were found to be resistant to oxacillin and were positive for the mecA gene. The isolates showed several multidrug-resistance patterns; the resistance rates to gentamicin, erythromycin, clindamycin, and trimethoprim/sulfamethoxazole were 89.7%, 86.2%, 75.9% and 72.4%, respectively. All isolates were susceptible to vancomycin, teicoplanin, rifampin, synercid, and ciprofloxacin. Several genotypic and phenotypic patterns were detected among the S. epidermidis isolates: antibiogram typing showed seven different patterns, one of which was shared by 65% of the isolates, whereas the most prevalent RAPD genotype was shared by only five S. epidermidis isolates, and did not correlate with antibiotic resistance phenotype. Conclusion: The diverse clonal origin of tested isolates indicates the presence of multiple S. epidermidis strains among neonates in the NICU setting. 2011 El Hafez et al.en_US
dc.identifier.doihttps://doi.org/
dc.identifier.issn20366590
dc.identifier.otherhttps://doi.org/
dc.identifier.urihttps://t.ly/py839
dc.language.isoEnglishen_US
dc.relation.ispartofseriesJournal of Infection in Developing Countries
dc.relation.ispartofseries5
dc.subjectMethicillin resistanceen_US
dc.subjectNICUen_US
dc.subjectSaudi Arabiaen_US
dc.subjectStaphylococcus epidermidisen_US
dc.subjectciprofloxacinen_US
dc.subjectclindamycinen_US
dc.subjectcotrimoxazoleen_US
dc.subjectdalfopristin plus quinupristinen_US
dc.subjecterythromycinen_US
dc.subjectgentamicinen_US
dc.subjectoxacillinen_US
dc.subjectrifampicinen_US
dc.subjectteicoplaninen_US
dc.subjectvancomycinen_US
dc.subjectantibiotic resistanceen_US
dc.subjectarticleen_US
dc.subjectbacterial geneen_US
dc.subjectbacterium identificationen_US
dc.subjectbacterium isolateen_US
dc.subjectblood cultureen_US
dc.subjectclinical articleen_US
dc.subjectepidemicen_US
dc.subjectfemaleen_US
dc.subjectgene identificationen_US
dc.subjectgenotypeen_US
dc.subjecthospital infectionen_US
dc.subjecthumanen_US
dc.subjectmaleen_US
dc.subjectmecA geneen_US
dc.subjectmethicillin resistant Staphylococcus epidermis infectionen_US
dc.subjectnewbornen_US
dc.subjectphenotypeen_US
dc.subjectrandom amplified polymorphic DNAen_US
dc.subjectreal time polymerase chain reactionen_US
dc.subjectSaudi Arabiaen_US
dc.subjectStaphylococcus infectionen_US
dc.subjecttracheal aspiration procedureen_US
dc.subjectwound assessmenten_US
dc.subjectBacterial Proteinsen_US
dc.subjectBlooden_US
dc.subjectCluster Analysisen_US
dc.subjectCross Infectionen_US
dc.subjectDisease Outbreaksen_US
dc.subjectFemaleen_US
dc.subjectGenotypeen_US
dc.subjectHumansen_US
dc.subjectInfant, Newbornen_US
dc.subjectMaleen_US
dc.subjectMethicillin Resistanceen_US
dc.subjectMolecular Epidemiologyen_US
dc.subjectMolecular Typingen_US
dc.subjectPolymorphism, Geneticen_US
dc.subjectRandom Amplified Polymorphic DNA Techniqueen_US
dc.subjectSaudi Arabiaen_US
dc.subjectStaphylococcal Infectionsen_US
dc.subjectStaphylococcus epidermidisen_US
dc.subjectTracheaen_US
dc.subjectUmbilicusen_US
dc.subjectBacteria (microorganisms)en_US
dc.subjectPosibacteriaen_US
dc.subjectStaphylococcus epidermidisen_US
dc.titleAn outbreak of methicillin resistant staphylococcus epidermidis among neonates in a hospital in Saudi Arabiaen_US
dc.typeArticleen_US
dcterms.isReferencedByWisplinghoff, H., Bischoff, T., Tallent, S.M., Seifert, H., Wenzel, R.P., Edmond, M.B., Nosocomial bloodstream infections in US hospitals: Analysis of 24,179 cases from a prospective nationwide surveillance study (2004) Clin Infect Dis, 39, pp. 309-317; Fluit, A.C., Schmitz, F.J., Verhoef, J., Multi-resistance to antimicrobial agents for the ten most frequently isolated bacterial pathogens (2001) Int J Antimicrob Agents, 18, pp. 147-160; Cimiotti, J.P., Wu, F., Larson, E., Emergence of resistant staphylococci on the hands of new graduate nurses (2004) Infect Control Hosp Epidemiol, 25, pp. 431-435; Krediet, T.G., Mascini, E.M., van Rooij, E., Vlooswijk, J., Paauw, A., Gerards, L.J., Fleer, A., Molecular epidemiology of coagulase-negative staphylococci causing sepsis in a neonatal intensive care unit over an 11-year period (2004) J Clin Microbiol, 42, pp. 992-995; Villari, P., Sarnataro, C., Iacuzio, L., Molecular epidemiology of Staphylococcus epidermidis in a neonatal intensive care unit over a three-year period (2000) J Clin Microbiol, 38, pp. 1740-1746; Haque, K.N., Chagia, A.H., Shaheed, M.M., Half a decade of neonatal sepsis, Riyadh, Saudi Arabia (1990) J Trop Pediatr, 36, pp. 20-23; Haque, K.N., Remo, C., Bahakim, H., Comparison of two types of intravenous immunoglobulins in the treatment of neonatal sepsis (1995) Clin Exp Immunol, 101, pp. 328-333; Babay, H.A., Twum-Danso, K., Kambal, A.M., Al-Otaibi, F.E., Bloodstream Infections in Pediatric Patients (2005) Saudi Med J, 26, pp. 1555-1561; Ahmed, M.M., Bahlas, S., Bacteriological profile and antimicrobial resistance patterns of clinical bacterial isolates in a University Hospital (2009) Travel Med Infect Dis, 7, pp. 235-238; Twum-Danso, K., Grant, C., Al-Suleiman, S.A., Abdel-Khader, S., Al-Awami, M.S., Al-Breiki, H., Taha, S., Wosornu, L., Microbiology of postoperative wound infection: A prospective study of 1770 wounds (1992) J Hosp Infect, 21, pp. 29-37; Wisplinghoff, H., Rosato, A.E., Enright, M.C., Noto, M., Craig, W., Archer, G.L., Related clones containing SCCmec type IV predominate among clinically significant Staphylococcus epidermidis isolates (2003) Antimicrob Agents Chemother, 47, pp. 3574-3579; Miragaia, M., Couto, I., Pereira, S.F., Kristinsson, K.G., Westh, H., Jarlov, J.O., Carrico, J., de Lencastre, H., Molecular characterization of methicillin-resistant Staphylococcus epidermidis clones: Evidence of geographic dissemination (2002) J Clin Microbiol, 40, pp. 430-438; Vermont, C.L., Hartwig, N.G., Fleer, A., de Man, P., Verbrugh, H., van den Anker, J., de Groot, R., van Belkum, A., Persistence of clones of coagulase-negative staphylococci among premature neonates in neonatal intensive care units: Two-center study of bacterial genotyping and patient risk factors (1998) J Clin Microbiol, 36, pp. 2485-2490; Raimundo, O., Heussler, H., Bruhn, J.B., Suntrarachun, S., Kelly, N., Deighton, M.A., Garland, S.M., Molecular epidemiology of coagulase-negative staphylococcal bacteraemia in a newborn intensive care unit (2002) J Hosp Infect, 51, pp. 33-42; Khashu, M., Osiovich, H., Henry, D., Al Khotani, A., Solimano, A., Speert, D.P., Persistent bacteremia and severe thrombocytopenia caused by coagulase-negative Staphylococcus in a neonatal intensive care unit (2006) Pediatrics, 117, pp. 340-348; Burnie, J.P., Naderi-Nasab, M., Loudon, K.W., Matthews, R.C., An epidemiological study of blood culture isolates of coagulase-negative staphylococci demonstrating hospital-acquired infection (1997) J Clin Microbiol, 35, pp. 1746-1750; Marsou, R., Idrissi, L., Benhammida, H., Zouhdi, M., Boudouma, M., Goldner, M., Relationship of Staphylococcal isolates in a Moroccan hospital by comparing phenotypical and genotypical tests (2001) Pathol Biol (Paris), 49, pp. 109-114; Abdallah, I.M., Araj, G.F., Matar, G.M., Abdelnour, G., Uwaydah, M., Abdelnoor, A.M., Polymerase chain reaction identification of coagulase-negative Staphylococci and of strain diversity and spread of Staphylococcus epidermidis in a major medical center in Lebanon (2006) Infect Control Hosp Epidemiol, 27, pp. 781-783; (2006), CLSI Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Performance Standards for Antimicrobial Disk Susceptibility Tests- Clinical Laboratory Standards Institute (CLSI) Seventh Edition Approved Standard M7-A7 CLSI Wayne, PA, USA; Ferreira, R.B., Iorio, N.L., Malvar, K.L., Nunes, A.P., Fonseca, L.S., Bastos, C.C., Santos, K.R., Coagulase-negative staphylococci: Comparison of phenotypic and genotypic oxacillin susceptibility tests and evaluation of the agar screening test by using different concentrations of oxacillin (2003) J Clin Microbiol, 41, pp. 3609-3614; Krediet, T.G., Jones, M.E., Janssen, K., Gerards, L.J., Fleer, A., Prevalence of molecular types and mecA gene carriage of coagulase-negative Staphylococci in a neonatal intensive care unit: Relation to nosocomial septicemia (2001) J Clin Microbiol, 39, pp. 3376-3378
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