Molecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitals

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorWasfi R.
dc.contributor.authorElkhatib W.F.
dc.contributor.authorAshour H.M.
dc.contributor.otherDepartment of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts
dc.contributor.otherGiza
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherAin Shams University
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Pharmacy Practice
dc.contributor.otherSchool of Pharmacy
dc.contributor.otherChapman University
dc.contributor.otherOrange
dc.contributor.otherCA
dc.contributor.otherUnited States; Department of Biological Sciences
dc.contributor.otherCollege of Arts and Sciences
dc.contributor.otherUniversity of South Florida St. Petersburg
dc.contributor.otherSt. Petersburg
dc.contributor.otherFL
dc.contributor.otherUnited States; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherCairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:29Z
dc.date.available2020-01-09T20:41:29Z
dc.date.issued2016
dc.descriptionScopus
dc.description.abstractKlebsiella pneumonia infection rates have increased dramatically. Molecular typing and virulence analysis are powerful tools that can shed light on Klebsiella pneumonia infections. Whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (MDR) patterns, 50% (18/36) indicated carpabenem resistance. Gene prevalence for the AcrAB efflux pump (82.14%) was more than that of the mdtK efflux pump (32.14%) in the MDR isolates. FimH-1 and mrkD genes were prevalent in wound and blood isolates. FimH-1 gene was prevalent in sputum while mrkD gene was prevalent in urine. Serum resistance associated with outer membrane protein coding gene (traT) was found in all blood isolates. IucC, entB, and Irp-1 were detected in 32.14%, 78.5% and 10.7% of MDR isolates, respectively. We used two Polymerase Chain Reaction (PCR) analyses: Enterobacterial Repetitive Intergenic Consensus (ERIC) and Random Amplified Polymorphic DNA (RAPD). ERIC-PCR revealed 21 and RAPD-PCR revealed 18 distinct patterns of isolates with similarity ?80%. ERIC genotyping significantly correlated with resistance patterns and virulence determinants. RAPD genotyping significantly correlated with resistance patterns but not with virulence determinants. Both RAPD and ERIC genotyping methods had no correlation with the capsule types. These findings can help up better predict MDR Klebsiella pneumoniae outbreaks associated with specific genotyping patterns. � The Author(s) 2016.en_US
dc.identifier.doihttps://doi.org/10.1038/srep38929
dc.identifier.doiPubMed ID 28004732
dc.identifier.issn20452322
dc.identifier.otherhttps://doi.org/10.1038/srep38929
dc.identifier.otherPubMed ID 28004732
dc.identifier.urihttps://t.ly/ndvqj
dc.language.isoEnglishen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofseriesScientific Reports
dc.relation.ispartofseries6
dc.subjectbacterial proteinen_US
dc.subjectclinical trialen_US
dc.subjectEgypten_US
dc.subjectfemaleen_US
dc.subjectgeneticsen_US
dc.subjectgenotyping techniqueen_US
dc.subjecthospitalen_US
dc.subjecthumanen_US
dc.subjectisolation and purificationen_US
dc.subjectKlebsiella infectionen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectmaleen_US
dc.subjectmulticenter studyen_US
dc.subjectmultidrug resistanceen_US
dc.subjectpathogenicityen_US
dc.subjectBacterial Proteinsen_US
dc.subjectDrug Resistance, Multiple, Bacterialen_US
dc.subjectEgypten_US
dc.subjectFemaleen_US
dc.subjectGenotyping Techniquesen_US
dc.subjectHospitalsen_US
dc.subjectHumansen_US
dc.subjectKlebsiella Infectionsen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectMaleen_US
dc.titleMolecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitalsen_US
dc.typeArticleen_US
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