Molecular characterization of Enterococcus spp. clinical isolates from Cairo, Egypt

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorHashem Y.A.
dc.contributor.authorYassin A.S.
dc.contributor.authorAmin M.A.
dc.contributor.otherDepartment of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherModern Sciences and Arts University
dc.contributor.otherGiza
dc.contributor.otherEgypt; Cairo University
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:57Z
dc.date.available2020-01-09T20:41:57Z
dc.date.issued2015
dc.descriptionScopus
dc.description.abstractPurpose: Enterococci are responsible for serious diseases such as bacteraemia, endocarditis and urinary tract infections. The ability of enterococci to cause such diseases is due to acquisition of certain virulence factors such as haemolysin, gelatinase and enterococcus surface protein. This study has been conducted to investigate the occurrence of virulence factors and resistance to various antibiotics with emphasis on vancomycin in the Enterococcus spp. Materials and Methods: Clinical specimens were collected and isolates were identified by proper microscopic, culture and biochemical tests. Susceptibility and degree of resistance of the isolates to various antibiotics were determined. Virulence factors were examined by phenotypic tests followed by molecular methods. Bioinformatics analysis was used to detect regions in the genomes that might have originated from horizontal gene transfer. Result: The presence or absence of virulence genes did not affect the pattern of antimicrobial resistance in Enterococcus isolates; consequently, no relationship was found between virulence factors and resistance to different antibiotics used. Bioinformatics analysis showed that the virulence genes were mainly transferred by transposons. Conclusion: Among the enterococci, environmental factors may interfere in the expression of virulence factors. Horizontal gene transfer plays an important role in the spread of resistance and virulence genes.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=20831&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.4103/0255-0857.148836
dc.identifier.doiPubMed ID 25657162
dc.identifier.issn2550857
dc.identifier.otherhttps://doi.org/10.4103/0255-0857.148836
dc.identifier.otherPubMed ID 25657162
dc.identifier.urihttps://t.ly/8pJ7W
dc.language.isoEnglishen_US
dc.publisherMedknow Publicationsen_US
dc.relation.ispartofseriesIndian Journal of Medical Microbiology
dc.relation.ispartofseries33
dc.subjectOctober University for Modern Sciences and Arts
dc.subjectجامعة أكتوبر للعلوم الحديثة والآداب
dc.subjectUniversity of Modern Sciences and Arts
dc.subjectMSA University
dc.subjectEgypten_US
dc.subjectEnterococcusen_US
dc.subjectResistanceen_US
dc.subjectUrinary tract infectionsen_US
dc.subjectVirulenceen_US
dc.subjectcytolysinen_US
dc.subjectgelatinaseen_US
dc.subjecthemolysinen_US
dc.subjectmembrane proteinen_US
dc.subjectvancomycinen_US
dc.subjectvirulence factoren_US
dc.subjectantiinfective agenten_US
dc.subjectantibiotic resistanceen_US
dc.subjectantibiotic sensitivityen_US
dc.subjectArticleen_US
dc.subjectbacterial geneen_US
dc.subjectbacterial genomeen_US
dc.subjectbacterial virulenceen_US
dc.subjectbacterium cultureen_US
dc.subjectbacterium examinationen_US
dc.subjectbacterium identificationen_US
dc.subjectbacterium isolateen_US
dc.subjectbiochemistryen_US
dc.subjectbioinformaticsen_US
dc.subjectEgypten_US
dc.subjectEnterococcusen_US
dc.subjectEnterococcus faeciumen_US
dc.subjectenvironmental factoren_US
dc.subjectenzyme activityen_US
dc.subjectgene expressionen_US
dc.subjecthorizontal gene transferen_US
dc.subjectmicroscopyen_US
dc.subjectminimum inhibitory concentrationen_US
dc.subjectnonhumanen_US
dc.subjectphenotypeen_US
dc.subjecttransposonen_US
dc.subjectclassificationen_US
dc.subjectdrug effectsen_US
dc.subjectEnterococcusen_US
dc.subjectgeneticsen_US
dc.subjectGram-Positive Bacterial Infectionsen_US
dc.subjecthumanen_US
dc.subjectisolation and purificationen_US
dc.subjectmicrobial sensitivity testen_US
dc.subjectmicrobiologyen_US
dc.subjectUrinary Tract Infectionsen_US
dc.subjectvirulenceen_US
dc.subjectAnti-Bacterial Agentsen_US
dc.subjectDrug Resistance, Bacterialen_US
dc.subjectEgypten_US
dc.subjectEnterococcusen_US
dc.subjectGene Transfer, Horizontalen_US
dc.subjectGenes, Bacterialen_US
dc.subjectGram-Positive Bacterial Infectionsen_US
dc.subjectHumansen_US
dc.subjectMicrobial Sensitivity Testsen_US
dc.subjectUrinary Tract Infectionsen_US
dc.subjectVirulenceen_US
dc.subjectVirulence Factorsen_US
dc.titleMolecular characterization of Enterococcus spp. clinical isolates from Cairo, Egypten_US
dc.typeArticleen_US
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