The effect of 3D-printed polycaprolactone (PCL) scaffold with different surface coatings on the immunogenicity of stem cells from human exfoliated deciduous teeth (SHED)

Simple item page
dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorAhmed N.E.-M.B.
dc.contributor.authorEl Azeem A.F.A.
dc.contributor.authorBayoumi F.S.
dc.contributor.otherDepartment of Oro-Dental Genetics
dc.contributor.otherMedical Research Centre of Excellence
dc.contributor.otherNational Research Centre
dc.contributor.otherCairo
dc.contributor.otherEgypt; Stem Cell Laboratory
dc.contributor.otherCenter of Excellence for Advanced Sciences
dc.contributor.otherNational Research Centre
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Microbiology and Immunology
dc.contributor.otherFaculty of Pharmacy
dc.contributor.otherOctober University of Modern Sciences and Arts (MSA)
dc.contributor.otherCairo
dc.contributor.otherEgypt; Department of Immunogenetics
dc.contributor.otherNational Research Centre
dc.contributor.otherCairo
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:41:01Z
dc.date.available2020-01-09T20:41:01Z
dc.date.issued2018
dc.descriptionScopus
dc.description.abstractBiomaterials have allowed many advances in the field of bone tissue engineering (BTE). Polycaprolactone (PCL) is an FDA degradable polymer that has been used for manufacturing scaffolds in bone tissue engineering. Different modifications have been made to PCL scaffold in order to improve its surface properties and osteoinductive abilities. It is essential that any modification of the engineered scaffold should avoid altering the properties of the seeded cells. Stem cells isolated from human exfoliated deciduous teeth (SHED)-similar to all other mesenchymal stem cells (MSCs)-do not express costimulatory molecules on their surface. These molecules are essential for the completion of T cell activation and therefore the lack of their expression accounts for the low immunogenicity of MSCs. In this study, SHED were isolated and seeded on 3D-printed PCL scaffolds which were either non-coated or coated with either nanohydroxyapatite (N-HAp) or multiwalled carbon nanotubes (MWCNTs). Cells cultured without scaffolds were used as a control. After three-day culture, all cells were collected and analyzed by flow cytometry for the expression of surface co-stimulatory molecules; CD40, CD80, and CD86. Results showed that different coating materials evidently affected the immune-genicity of the seeded SHED. The expression of CD40, CD80, and CD86 markers was significantly higher in cells seeded on MWCNTs/PCL scaffolds, followed by cells seeded on N-HAp/PCL scaffolds when compared to cells seeded on non-coated PCL scaffolds. On the other hand, cells seeded on non-coated PCL scaffolds showed no significant difference in their expression to the specified markers when compared to the control group. The data presented in this study are significant when considering allogeneic MSCs treatments in order to avoid immune rejection. � Nova Science Publishers, Inc.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=19700169102&tip=sid&clean=0
dc.identifier.doihttps://doi.org/
dc.identifier.issn15568539
dc.identifier.otherhttps://doi.org/
dc.identifier.urihttps://t.ly/JXK7Y
dc.language.isoEnglishen_US
dc.publisherNova Science Publishers, Inc.en_US
dc.relation.ispartofseriesJournal of Stem Cells
dc.relation.ispartofseries13
dc.subject3D printingen_US
dc.subjectImmunogenicityen_US
dc.subjectPolycaprolactoneen_US
dc.subjectScaffoldsen_US
dc.subjectStem cellsen_US
dc.subjectB7 antigenen_US
dc.subjectCD40 antigenen_US
dc.subjectCD86 antigenen_US
dc.subjectfluorochromeen_US
dc.subjectmulti walled nanotubeen_US
dc.subjectpolycaprolactoneen_US
dc.subjectArticleen_US
dc.subjectdeciduous toothen_US
dc.subjectflow cytometryen_US
dc.subjecthumanen_US
dc.subjectimmunogenicityen_US
dc.subjectin vitro studyen_US
dc.subjectmesenchymal stem cellen_US
dc.subjectprotein expressionen_US
dc.subjectthree dimensional printingen_US
dc.titleThe effect of 3D-printed polycaprolactone (PCL) scaffold with different surface coatings on the immunogenicity of stem cells from human exfoliated deciduous teeth (SHED)en_US
dc.typeArticleen_US
dcterms.isReferencedByYang, F., Wang, J., Hou, J., Guo, H., Liu, C., Bone regeneration using cell-mediated responsive degradable PEG-based scaffolds incorporating with rhBMP-2 (2013) Biomaterials, 34, pp. 1514-1528; Athanasiou, V.T., Papachristou, D.J., Panagopoulos, A., Saridis, A., Scopa, C.D., Megas, P., Histological comparison of autograft, allograft-DBM, xenograft, and synthetic grafts in a trabecular bone defect: An experimental study in rabbits (2010) Med Sci Monit, 16 (1), pp. 24-31; Rawashdeh, M.A., Morbidity of iliac crest donor site following open bone harvesting in cleft lip and palate patients (2008) Int J Oral Maxillofac Surg, 37, pp. 223-227; Al-Ahmady, H.H., Abd Elazeem, A.F., Ahmed, N.E.B., Shawkat, W.M., Elmasry, M., Abdelrahman, M.A., Abderazik, M.A., Combining autologous bone marrow mononuclear cells seeded on a collagen sponge with nano-hydroxyapatite, and platelet-rich fibrin: Reporting a novel strategy for alveolar cleft bone regeneration (2018) J Craniomaxillofac Surg, 46, pp. 1593-1600; Wang, X., Li, G., Guo, J., Yang, L., Liu, Y., Sun, Q., Li, R., Yu, W., Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet-rich fibrin granules for bone regeneration in a rabbit calvarial critical-size defect model (2017) Exp Ther Med, 13 (5), pp. 1891-1899; Uchiyama, H., Yamato, M., Sasaki, R., Sekine, H., Yang, J., Ogiuchi, H., Ando, T., Okano, T., In vivo 3D analysis with micro-computed tomography of rat calvaria bone regeneration using periosteal cell sheets fabricated on temperature-responsive culture dishes (2011) J Tissue Eng Regen Med, 5, pp. 483-490; Seitz, H., Rieder, W., Irsen, S., Leukers, B., Tille, C., Three?dimensional printing of porous ceramic scaffolds for bone tissue engineering (2005) J. Biomed. Mater. Res, 74B, pp. 782-788; McNeil, S.E., Griffiths, H.R., Perrie, Y., Polycaprolactone fibers as a potential delivery system for collagen to support bone regeneration (2011) Curr Drug Deliv, 8, pp. 448-455; Hiep, N.J., Khon, H.C., Hai, N.D., Byong-Taek, L., Toi, V.V., Hung, L.T., Biocompatibility of PCL/PLGA-BCP porous scaffold for bone tissue engineering applications (2017) J Biomater Sci Polym, 28 (9), pp. 864-878; Salerno, A., Fernndez-Gutirrez, M., Romn Del Barrio, J.S., Domingo, C., Bio-safe fabrication of PLA scaffolds for bone tissue engineering by combining phase separation, porogen leaching and scCO2 drying (2014) J. Supercrit. Fluid., 97, pp. 238-246; Baolin, G., Ma, P.X., Synthetic biodegradable functional polymers for tissue engineering: A brief review (2014) Sci China Chem, 57 (4), pp. 490-500; Petrie, A.C., Cooper, J.J., Sefcik, L.S., Tholpady, S.S., Ogle, R.C., Botchwey, E.A., Osteogenic differentiation of dura mater stem cells cultured in vitro on three-dimensional porous scaffolds of poly (Epsilon-Caprolactone) fabricated via co-extrusion and gas foaming (2008) Acta Biomater, 4, pp. 1187-1197; Kim, H.J., Lee, J.H., Im, G.I., Chondrogenesis using mesenchymal stem cells and PCL scaffolds (2010) J. Biomed. Mater. Res. A, 92, pp. 659-666; Izquierdo, R., Garcia-Giralt, N., Rodriguez, M.T., Caceres, E., Garcia, S.J., Gomez, R.J., Monleon, M., Suay, J., Biodegradable PCL scaffolds with an interconnected spherical pore network for tissue engineering (2008) J. Biomed. Mater. Res. A, 85, pp. 25-35; Luong-Van, E., Grondahl, L., Chua, K.N., Leong, K.W., Nurcombe, V., Cool, S.M., Controlled release of heparin from poly (Epsilon-Caprolactone) electrospun fibers (2006) Biomaterials, 27, pp. 2042-2050; Zelenkov, T., Mora, M.J., Barresi, A.A., Granero, G.E., Fissore, D., On the production of chitosan-coated polycaprolactone nanoparticles in a confined impinging jet reactor (2018) J Pharm Sci J, 107 (4), pp. 1157-1166; Sousa, I., Mendes, A., Brtolo, B.J., PCL scaffolds with collagen bioactivator for applications in tissue engineering (2013) Procedia Eng, 59, pp. 279-284; Miura, M., Gronthos, S., Zhao, M., Lu, B., Fisher, L.W., Robey, P.G., Shi, S., SHED: Stem cells from human exfoliated deciduous teeth (2003) Proc Natl Acad Sci USA, 100, pp. 5807-5812; Behnia, A., Haghighat, A., Talebi, A., Nourbakhsh, N., Heidari, F., Transplantation of stem cells from human exfoliated deciduous teeth for bone regeneration in the dog mandibular defect (2014) World J Stem Cells, 6 (4), pp. 505-510; Vakhrushev, I.V., Antonov, E.N., Popova, A.V., Konstantinova, E.V., Karalkin, P.A., Kholodenko, I.V., Lupatov, A.Y., Yarygin, K.N., Design of tissue engineering implants for bone tissue regeneration of the basis of new generation poly-lactoglycolide scaffolds and multipotent mesenchymal stem cells from human exfoliated deciduous teeth (SHED cells) (2012) Bull Exp Biol Med, 153 (1), pp. 143-147; Nakajima, K., Kunimatsu, R., Ando, K., Ando, T., Hayashi, Y., Kihara, T., Hiraki, T., Tanimoto, K., Comparison of the bone regeneration ability between stem cells from human exfoliated deciduous teeth, human dental pulp stem cells and human bone marrow mesenchymal stem cells (2018) Biochem Biophys Res Commun, 497 (3), pp. 876-882; Le Blanc, K., Tammik, L., Sundberg, B., Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex (2003) Scand J Immunol, 57 (1), pp. 11-20; Aggarwal, S., Pittenger, M.F., Human mesenchymal stem cells modulate allogeneic immune cell responses (2005) Blood, 105 (4), pp. 1815-1822; Ryan, J.M., Barry, F.P., Murphy, J.M., Mesenchymal stem cells avoid allogeneic rejection (2005) J Inflamm, 2 (1), p. 8; Di Nicola, M., Carlo-Stella, C., Magni, M., Milanesi, M., Longoni, P.D., Matteucci, P., Grisanti, S., Gianni, A.M., Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli (2002) Blood, 99 (10), pp. 3838-3843; Magee, C.N., Boenisch, O., Najafian, N., The role of costimulatory molecules in directing the functional differentiation of allo-reactive t helper cells (2012) Am J Transplant, 12 (10), pp. 2588-2600; Abouelsayed, A., Ebrahim, M.R., El Hotaby, W., Hassan, S.A., Al-Ashkar, E., Terhertz acoustic phonon detection from a compact surface layer of spherical nanoparticles powder mixture of aluminum, alumina and multi-walled carbon nanotube (2017) Spectrochim. Acta a Mol. Biomol. Spectrosc, 185 (2017), pp. 179-187; Tarafder, S., Koch, A., Jun, Y., Chou, C., Awadallah, M.R., Lee, C.H., Micro-precise spatiotemporal delivery system embedded in 3D printing for complex tissue regeneration (2016) Biofabrication, 8; Nyberg, E., Rindone, A., Dorafshar, A., Grayson, W.L., Comparison of 3D-printed poly-epsilon-caprolactone scaffolds functionalized with tricalcium Phosphate, hydroxyapatite, bio-oss, or decellularized Bone Matrix (2016) Tissue Eng. Part A, 23, pp. 503-514; Jeong, C.G., Hollister, S.J., A comparison of the influence of material on in vitro cartilage tissue engineering with PCL, PGS, and POC 3D scaffold architecture seeded with chondrocytes (2010) Biomaterials, 31, pp. 4304-4312; Ho, C.M., Mishra, A., Lin, P.T., Ng, S.H., Yeong, W.Y., Kim, Y.J., Yoon, Y.J., 3D printed polycaprolactone carbon nanotube composite scaffolds for cardiac tissue engineering (2016) Macromol. Biosci., 17; Park, S.A., Lee, H.J., Kim, K.S., Lee, S.J., Lee, J.T., Kim, S.Y., Chang, N.H., In vivo evaluation of 3D-printed poly-poly-caprolactone scaffold implantation combined with-TCP powder for alveolar bone augmentation in a beagle defect model (2018) Materials, 11 (2), p. 238. , caprolactone; Heydari, Z., Mohebbi-Kalhori, D., Afarani, M.S., Engineered electrospun polycaprolactone (PCL)/ octa-calcium phosphate (OCP) scaffold for bone tissue engineering (2017) Mater Sci Eng C Mater Biol Appl, 81, pp. 127-132; Malikmammadov, E., Tanir, T.E., Kiziltay, A., Hasirci, V., Hasirci, N., PCL and PCL-based materials in biomedical applications (2017) J Biomater Sci Polym Ed, 29 (7), pp. 863-893; Kinoshita, Y., Maeda, M., Recent developments of functional scaffolds for craniomaxillofacial bone tissue engineering applications (2013) Sci. World J, p. 21; Wei, G., Ma, P.X., Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering (2004) Biomaterials, 25 (19), pp. 4749-4757; Zancanela, D.C., de Faria, A.N., Simao, A.M., Goncalves, R.R., Ramos, A.P., Ciancaglini, P., Multi and single walled carbon nanotubes: Effects on cell responses and bio-mineralization of osteoblasts cultures (2016) J Mater Sci Mater Med, 27 (3), p. 62; Usui, Y., Aoki, K., Narita, N., Murakami, N., Nakamura, I., Nakamura, K., Ishigaki, N., Saito, N., Carbon nanotubes with high bone tissue compatibility and bone formation acceleration effects (2008) Small, 4 (2), pp. 240-246; Li, X., Liu, H., Niu, X., Yo, B., Fan, Y., Feng, Q., Cui, F., Watari, F., The use of carbon nanotubes to induce osteogenic differentiation of human adipose-derived MSCs in vitro and ectopic bone formation in vivo (2012) Biomaterials, 33 (19), pp. 4818-4827; Wu, D., Zhang, Y., Zhang, M., Yu, W., Selective localization of multiwalled carbon nanotubes in poly (?-capro-lactone)/polylactide blend (2009) Biomacromolecules, 10 (2), pp. 417-424; Chen, G.X., Kim, H.S., Park, B.H., Synthesis of poly (L-lactide) functionalized multiwalled carbon nanotubes by ring opening polymerization (2007) Macromol Chem Phys, 208 (4), pp. 389-398; Mattioli-Belmonte, M., Vozzi, G., Whulanza, Y., Seggiani, M., Fantauzzi, V., Orsini, G., Ahluwalia, A., Tuning polycaprolactone�carbon nanotube composites for bone tissue engineering scaffolds (2012) Mater Sci Eng C, 32 (2), pp. 152-159; Kim, J.W., Shin, K.H., Koh, Y.H., Hah, M.J., Moon, J., Kim, H.E., Production of poly("-caprolactone)/hydroxyapatite composite scaffolds with a tailored macro/micro-porous structure, high mechanical properties, and excellent bioactivity (2017) Materials, 10, p. 1123; Liu, H., Lu, K., Macary, P.A., Wong, K.L., Heng, A., Cao, T., Kemeny, D.M., Soluble molecules are key in maintaining the immunomodulatory activity of murine mesenchymal stromal cells (2012) J Cell Sci, 125 (1), pp. 200-208; Patrikoski, M., Sivula, J., Huhtala, H., Helminen, M., Salo, F., Mannerstrom, B., Miettinena, S., Different culture conditions modulate the immunological properties of adipose stem cells (2014) Stem Cells Transl Med, 3 (10), pp. 1220-1230; Ahmed, N.E.-B., Murakami, M., Kaneko, S., Nakashima, M., The effects of hypoxia on the stemness properties of human dental pulp stem cells (DPSCs) (2016) Sci Rep, 6
dcterms.sourceScopus

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
avatar_scholar_128.png
Size:
2.73 KB
Format:
Portable Network Graphics
Description:
Download

Collections

Faculty Of Pharmacy Research Paper