In vitro mesenchymal stem cells differentiation into hepatocyte-like cells in the presence and absence of 3D microenvironment
| dc.Affiliation | October University for modern sciences and Arts (MSA) | |
| dc.contributor.author | El-Hamid S.A. | |
| dc.contributor.author | Mogawer A. | |
| dc.contributor.other | Department of Clinical Pathology | |
| dc.contributor.other | Kasr El-Aini School of Medicine | |
| dc.contributor.other | Cairo University | |
| dc.contributor.other | Cairo | |
| dc.contributor.other | Egypt; Department of Biotechnology | |
| dc.contributor.other | MSA University | |
| dc.contributor.other | 6 October | |
| dc.contributor.other | Giza | |
| dc.contributor.other | Egypt | |
| dc.date.accessioned | 2020-01-09T20:42:14Z | |
| dc.date.available | 2020-01-09T20:42:14Z | |
| dc.date.issued | 2014 | |
| dc.description | Scopus | |
| dc.description.abstract | Our study was aimed to select the best growth factor cocktail for differentiation of mesenchymal stem cells (MSCs) into hepatocytes-like cells (HLCs) in absence and presence of three dimensional (3D) microenvironment. Ninety milliliters of bone marrow was aspirated from the iliac bone for separation of MSCs. This study was conducted on 20 patients with advanced liver cirrhosis. They were divided into two groups. Group I; MSCs were plated onto 96-well plates without microenvironment (control group). Group II; MSCs were plated onto 96-well plates with 3D microenvironment. Surface expression of CD271, CD29, and CD34 were analyzed using flow cytometry. MSCs were differentiated in vitro into HLCs by adding four growth factor cocktails in presence and absence of 3D microenvironment. Hepatogenesis was assessed by immunohistochemical analysis of OV6, ?-fetoprotein, albumin, and cytokeratin 18 expressions. There was statistically significant increase in the expression of CD271 and CD29 after MSCs culture (P < 0.001). Heterogeneous cell population composed of MSCs and differentiated HLCs were encountered (40 % hepatocytes and 60 % MSCs). Furthermore, our results showed that growth factor cocktails 1 and 2 gave the best result for differentiation of MSCs into HLCs. MSCs could be feasible, readily available, and novel source for differentiation of MSCs into HLCs for future therapeutic implication. � 2013 Springer-Verlag London. | en_US |
| dc.description.uri | https://www.scimagojr.com/journalsearch.php?q=25930&tip=sid&clean=0 | |
| dc.identifier.doi | https://doi.org/10.1007/s00580-013-1741-5 | |
| dc.identifier.doi | PubMed ID : | |
| dc.identifier.issn | 16185641 | |
| dc.identifier.other | https://doi.org/10.1007/s00580-013-1741-5 | |
| dc.identifier.other | PubMed ID : | |
| dc.identifier.uri | https://t.ly/6x30Z | |
| dc.language.iso | English | en_US |
| dc.publisher | Springer-Verlag London Ltd | en_US |
| dc.relation.ispartofseries | Comparative Clinical Pathology | |
| dc.relation.ispartofseries | 23 | |
| dc.subject | 3D microenvironment | en_US |
| dc.subject | Differentiation | en_US |
| dc.subject | Growth factors | en_US |
| dc.subject | Hepatocyte-like cells | en_US |
| dc.subject | Mesenchymal stem cells | en_US |
| dc.title | In vitro mesenchymal stem cells differentiation into hepatocyte-like cells in the presence and absence of 3D microenvironment | en_US |
| dc.type | Article | en_US |
| dcterms.isReferencedBy | Allen, K.J., Buck, N.E., Williamson, R., Stem cells for the treatment of liver disease (2005) Transpl Immunol, 15, pp. 99-112; Baddoo, M., Hill, K., Wilkinson, R., Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection (2003) J Cell Biochem, 89, pp. 1235-1249; Block, G.D., Locker, J., Bowen, W.C., Petersen, B.E., Katyal, S., Strom, S.C., Population expansion, clonal growth, and specific differentiation patterns in primary cultures of hepatocytes induced by HGF/SF, EGF and TGF ? in a chemically defined (HGM) medium (1996) J Cell Biol, 132 (6), pp. 1133-1149; Boiret, N., Rapatel, C., Veyrat-Masson, R., Characterization of nonexpanded mesenchymal progenitor cells from normal adult human bone marrow (2005) Exp Hematol, 33, pp. 219-225; Bruno, S., Grange, C., Deregibus, M.C., Mesenchymal stem cell-derived microvesicles protect against acute tubular injury (2009) J Am Soc Nephrol, 20, pp. 1053-1067; B�hring, H.J., Battula, V.L., Treml, S., Novel markers for the prospective isolation of human MSC (2007) Ann N Y Acad Sci, 1106, pp. 262-271; Chivu, M., Dima, S.O., Stancu, C.I., In vitro hepatic differentiation of human bone marrow mesenchymal stem cells under differential exposure to liver-specific factors (2009) Transl Res, 154 (3), pp. 122-132; Chop, M., Li, Y., Treatment of neural injury with marrow stromal cells (2002) Lancet Neurol, 1, pp. 92-100; Christoforou, N., Gearhart, J.D., Stem cells and their potential in cell-based cardiac therapies (2007) Prog Cardiovasc Dis, 49, pp. 396-413; Darlington, G.J., Molecular mechanisms of liver development and differentiation (1999) Curr Opin Cell Biol, 11 (6), pp. 678-682; Darwish, M.A., Faris, R., Darwish, N., Hepatitis C and cirrhotic liver disease in the Nile delta of Egypt: a community-based study (2001) Am J Trop Med Hyg, 64, pp. 147-153; Forbes, J.S., Stem cell therapy for liver disease-choosing the right tools for the job (2008) Gut, 57, pp. 153-155; Kazemnejad, S., Allameh, A., Seoleimani, M., Functional hepatocyte-like cells derived from human bone marrow mesenchymal stem cells on a novel 3-dimensional biocompatible nanofibrous scaffold (2008) Int J Artif Organs, 31 (6), pp. 500-507; Li, J., Tao, R., Wu, W., 3D PLGA scaffolds improve differentiation and function of bone marrow mesenchymal stem cell-derived hepatocytes (2010) Stem Cells Dev, 19 (9), pp. 1427-1436; Libbrecht, L., Desmet, V., van Damme, B., The immunohistochemical phenotype of dysplastic foci in human liver: correlation with putative progenitor cells (2000) J Hepatol, 33, pp. 76-84. , GJ; Lin, N., Lin, J., Bo, L., Differentiation of bone marrow-derived mesenchymal stem cells into hepatocyte-like cells in an alginate scaffold (2010) Cell Prolif, 43 (5), pp. 427-434; Oh, S.H., Miyazaki, M., Kouchi, H., Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro (2000) Biochem Biophys Res Commun, 279 (2), pp. 500-504; Oyagi, S., Hirose, M., Kojima, M., Therapeutic effect of transplanting HGF-treated bone marrow mesenchymal cells into CCl4-injured rats (2006) J Hepatol, 44, pp. 742-748; Piryaei, A., Valojerdi, M.R., Shahsavani, M., Differentiation of bone marrow-derived mesenchymal stem cells into hepatocyte-like cells on nanofibers and their transplantation into a carbon tetrachloride-induced liver fibrosis model (2011) Stem Cell Rev, 7 (1), pp. 103-118; Schindler, M., Nur-E-Kamal, A., Ahmed, I., Living in three dimensions: 3D nanostructured environments for cell culture and regenerative medicine (2006) Cell Biochem Biophys, 45 (2), pp. 215-227; Schwartz, R.E., Reyes, M., Koodie, L., Jiang, Y., Blackstad, M., Lund, T., Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells (2002) J Clin Invest, 109 (10), pp. 1291-1302; Silva, W.A., Covas, D.T., Panepucci, R.A., The profile of gene expression of human marrow mesenchymal stem cells (2003) Stem Cells, 21, pp. 661-669; Stefania, L., Pietro, A., Regenerative medicine and liver injury: what role for bone marrow derived stem cells? (2007) Curr Stem Cell Res Ther, 2, pp. 83-88; Svegliati-Baroni, G., De Minicis, S., Marzioni, M., Hepatic fibrogenesis in response to chronic liver injury: novel insights on the role of cell-to-cell interaction and transition (2008) Liver Int, 28 (8), pp. 1052-1064; Wang, X., Willenbring, H., Akkari, Y., Cell fusion is the principal source of bone-marrow-derived hepatocytes (2003) Nature, 422, pp. 897-901; Xiao, J.C., Ruck, P., Adam, A., Small epithelial cells in human liver cirrhosis exhibit features of hepatic stem like cells: immunohistochemical, electron microscopic and immunoelectron microscopic findings (2003) Histopathology, 42, pp. 141-149; Xie, J.M., Chen, J.F., Gao, Y., Hepatocyte growth factor and fibroblast growth factor-4-induced differentiation of human bone marrow mesenchymal stem cells into hepatocyte-like cells in vitro (2010) Cell Prolif, 43 (5), pp. 427-434; Yagi, K., Kojima, M., Oyagi, S., Application of mesenchymal stem cells to liver regenerative medicine (2008) Yakugaku Zasshi, 128 (1), pp. 3-9 | |
| dcterms.source | Scopus |