Genetic diversity analysis of North Africa’s barley using SSR markers

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Date

2012

Journal Title

Journal ISSN

Volume Title

Type

Article

Publisher

Elsevier

Series Info

Journal of Genetic Engineering and Biotechnology;Volume 10, Issue 1, June 2012, Pages 13-21

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Abstract

It was demonstrated that some North Africa barley accessions have diverse tolerance sources for abiotic stresses and a good nutritional quality, but the studies done were incomplete since they were realized separately in each country apart. To implement a more complete analysis, 31 barley accessions originated from North Africa (Algeria, Tunisia and Egypt) were analyzed using 11 SSR markers selected from the seven barley linkage groups for studying the genetic diversity among these chosen barley accessions. Over the 11 SSR markers, a total of 478 reproducible bands were scored with an average of 2.13 alleles/primer and the average polymorphism information content of 0.5. Genetic distance analysis of the 31 accessions showed a large genetic diversity and high number of different groups. The most accessions are clustered according to their eco-geographical origin, according to their pedigree and agronomic characters or according to the caryopsis character (hulled or naked caryopsis). This high number of obtained groups is an invaluable aid in crop improvement strategies and confirms the opinion suggesting that North Africa could be a secondary center of origin of barley. The various growing conditions and the multiple uses of barley in each country may be the cause of the large variability of the barley germplasm in each region.

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Keywords

Hordeum, Genetic diversity, SSR markers, North Africa, Agronomic character, Geographical origin

Citation

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