Protective effect of Echinops galalensis against CCl4-induced injury on the human hepatoma cell line (Huh7)
Date
2013
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Elsevier
Series Info
Phytochemistry Letters;6
Doi
Scientific Journal Rankings
Abstract
Phytochemical investigation of the flowering aerial parts of Echinops galalensis (Asteraceae) led to the
isolation of a new taraxasteryl triterpene, 3b-acetoxy-taraxast-12, 20(30)-diene-11a-21a-diol (1),
together with nine known metabolites, a-amyrin (2), b-sitosterol (3), erythrodiol (4), lup-20(29)-ene-
1,3-diol (5), 1,5-dicaffeoylquinic acid (6), 3,5-dicaffeoylquinic acid (7), 3,4-dicaffeoylquinic acid (8), 4,5-
dicaffeoylquinic acid (9) and apigenin-7-O-b-D-glucoside (10). The structure of the new compound was
determined by comprehensive analyses of their 1D and 2D NMR, mass spectral (HR-EI) data and
comparison with previously known analogs. The effect of the methanol extract of E. galalensis, its
fractions as well as compounds (1–10) on human hepatoma cell line (Huh7) was evaluated according to
aspartate aminotransferase (AST), alanine transaminase (ALT), superoxide dismutase (SOD) activities
and malondialdehyde (MDA) level before and after exposure of the cells to carbon tetrachloride (CCl4). It
was found that pre-treatment of human hepatoma cell line (Huh7) with the tested samples (100 mg/ml)
prior to CCl4 challenge protected against cell injury. The protective effect of E. galalensis was suggested to
be mediated, at least partly, by its antioxidant activity.
Description
MSA Google Scholar
Keywords
University of Echinops galalensis, Hepatoprotective
Citation
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