Molecular phylogeny and identification of the Egyptian wasps (Hymenoptera: Vespidae) based on COI mitochondrial gene sequences
Loading...
Date
2018-04
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Springer
Series Info
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL;Volume: 28
Doi
Scientific Journal Rankings
Abstract
The Hymenoptera is one of the vital and biggest insect orders comprising the bees, wasps, sawflies, and ants. Wasps are important to natural and biological pest control because they are predators or parasitoids of pest arthropods. This study investigated the genetic diversity among the three wasps, Vespa orientalis Linnaeus, Polistes bucharensis Erichson, and Polistes mongolicus du Buysson, collected from three different governorates in Egypt, using cytochrome oxidase subunit I (COI) DNA barcoding. PCR was performed to amplify COI fragment. The amplified COI regions (710 bp) were sequenced and analyzed. All novel nucleotide sequences of COI gene were deposited into the GenBank database. The genetic distances were estimated using Kimura two-parameter model. In spite of the wide geographical range, minor genetic diversity was observed between some populations of the three wasp species, revealing unrestricted gene flow between them. Phylogenetic relationship analysis was performed, using maximum likelihood (ML) method. The results of the phylogenetic analyses recovered P. bucharensis more closely related to P. dominula and P. gallicus. P. mongolicus collected from Menofia Governorate formed a distinct branch with 99% support. V. orientalis was sister to the yellowjacket Dolichovespula adulterine, with 84% support. It can be concluded that DNA barcode is a powerful tool for rapid and accurate identification of Egyptian wasp species.
Description
Accession Number: WOS:000436822700001
Keywords
University for COI, Egypt, DIVERSITY, CLASSIFICATION, LARVAE, COLEOPTERA;, POPULATIONS, VESPINAE, POLISTES, PAPER WASPS, INSECTA HYMENOPTERA, DNA BARCODES, Phylogeny, Hymenoptera, Vespidae
Citation
Ahrens D, Monaghan MT, Vogler AP (2007) DNA-based taxonomy for associating adults and larvae in multi-species assemblages of chafers (Coleoptera: Scarabaeidae). Mol Phylogenet Evol 44:436–449 CrossRefPubMedGoogle Scholar Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W, Lipman D (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389e3402 CrossRefGoogle Scholar Arens W (2011) Die sozialen Faltenwespen der Peloponnes (Hymenoptera: Vespidae, Polistinae), mit Beschreibung einer neuen Polistes-Art und einem regionalen Polistes-Bestimmungsschlüssel. Linzer Biologische Beiträge 43(1):443–481 Google Scholar Boyer S, Blakemore RJ, Wratten SD (2011) An integrative taxonomic approach to the identification of three new New Zealand endemic earthworm species (Acanthodrilidae, Octochaetidae: Oligochaeta). Zootaxa 2994:21–32 Google Scholar Buck M, Cobb TP, Stahlhut JK, Hanner RH (2012) Unravelling cryptic species diversity in eastern Nearctic paper wasps, Polistes (Fuscopolistes), using male genitalia, morphometrics and DNA barcoding, with descriptions of two new species (Hymenoptera: Vespidae). Zootaxa 3502:1–48 Google Scholar Burns JM, Janzen DH, Hallwachs W (2010) Of many similar species in the Neotropical genus Porphyrogenes (Lepidoptera: Hesperiidae), a new one, repeatedly reared in Costa Rica, is relatively distinct. Proc Entomol Soc Wash 112:32–42 CrossRefGoogle Scholar Carpenter J, Kojima JI, Villemant C (2013) Phylogeny of hornets: a total evidence approach (Hymenoptera, Vespidae, Vespinae, Vespa). J Hymenopt Res 32:1 CrossRefGoogle Scholar Carpenter JM (1987) Phylogenetic relationships and classification of the Vespinae (Hymenoptera: Vespidae). Syst Entomol 12:413–431 CrossRefGoogle Scholar Cervo R, Dapporto L, Beani L, Strassmann JE, Turillazzi S (2008) On status badges and quality signals in the paper wasp Polistes dominulus: body size, facial colour patterns and hierarchical rank. Proc R Soc Lond B 275:1189–1196 https://doi.org/10.1098/rspb.2007.1779 CrossRefGoogle Scholar Cruaud A, Jabbour-Zahab R, Genson G et al (2010) Laying the foundations for a new classification of Agaonidae (Hymenoptera: Chalcidoidea), a multilocus phylogenetic approach. Cladistics 26:359–387 Google Scholar Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17(6):368–376 CrossRefPubMedGoogle Scholar Grissell E (2010) Bees, wasps, and ants: the indispensable role of Hymenoptera in gardens, 1st edn. Timber Press, Portland, p 11 20-21 Google Scholar Haddad NJ, Dvořák L, Adwan O, Mdanat H, Batayna A (2007) New data on Vespid wasp fauna of Jordan (Hymenoptera, Vespidae) Linzer biol. Beitr 39(1):137–142 Google Scholar Haddad NJ, Fuchs S, Haddaden J, Kopelke JP (2005) Record of Sphecophaga vesparum Curtis, a natural enemy of Vespa orientalis in northern Jordan. Zool Middle East 35(1):114–116 Google Scholar Hayashi M, Sota T (2010) Identification of elmid larvae (Coleoptera: Elmidae) from Sanin District of Honshu, Japan, based on mitochondrial DNA sequences. Entomol Sci 13:417–424 CrossRefGoogle Scholar Hebert PDN, Cywinska A, Ball SL, de Waard JR (2003b) Biological identifications through DNA barcodes. Proc R Soc B Biol Sci 270:313–321 CrossRefGoogle Scholar Hebert PDN, deWaard JR, Landry JF (2010) DNA barcodes for 1/1000 of the animal kingdom. Biol Lett 6:359–362 CrossRefPubMedGoogle Scholar Hebert PDN, Penton EH, Burns JM, Janzen DH, Hallwachs W (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proc Natl Acad Sci U S A 101:14 812–14 817 CrossRefGoogle Scholar Hebert PDN, Ratnasingham S, de Waard JR (2003a) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc R Soc Lond Ser B 270:S96–S99 CrossRefGoogle Scholar Hunt JH (2007) The evolution of social wasps. Oxford University Press, USA, p 261 CrossRefGoogle Scholar International Barcode of Life 2010. Lepidoptera barcode of life. Available from URL: http://www.lepbarcoding.org/. [Cited 15 Oct 2010] Google Scholar Jandt JM, Tibbetts EA, Toth AL (2014) Polistes paper wasps: a model genus for the study of social dominance hierarchies. Insect Soc 61(1):11–27 https://doi.org/10.1007/s00040-013-0328-0 CrossRefGoogle Scholar Kaila L, Ståhls G (2006) DNA barcodes: evaluating the potential of COI to differentiate closely related species of Elachista (Lepidoptera: Gelechioidea: Elachistidae) from Australia. Zootaxa 1170:1–26 Google Scholar Kathirithamby J, Hayward A, McMahon DP et al (2010) Conspecifics of a heterotrophic heteronomous species of Strepsiptera (Insecta) are matched by molecular characterization. Syst Entomol 35:234–242 CrossRefGoogle Scholar Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 CrossRefPubMedGoogle Scholar Kishino H, Miyata T, Hasegawa M (1990) Maximum likelihood inference of protein phylogeny and the origin of chloroplasts. J Mol Evol 31(2):151–160 CrossRefGoogle Scholar Kress WJ, Erickson DL, Jones FA et al (2009) Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama. Proc Natl Acad Sci U S A 106:18621–18626 CrossRefPubMedPubMedCentralGoogle Scholar Kumar LS, Sawant AS, Gupta VS, Ranjekar PK (2001) Genetic variation in Indian populations of Scirpophaga incertulas as revealed by RAPD-PCR analysis. Biochem Genet 39(1–2):43–57 CrossRefPubMedGoogle Scholar Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874 CrossRefPubMedGoogle Scholar Langhoff P, Authier A, Buckley TR, Dugdale JS, Rodrigo A, Newcomb RD (2009) DNA barcoding of the endemic New Zealand leafroller moth genera, Ctenopseustis and Planotortrix. Mol Ecol Resour 9:691–698 CrossRefPubMedGoogle Scholar Li YW, Zhou X, Feng G et al (2010) COI and ITS2 sequences delimit species, reveal cryptic taxa and host specificity of fig-associated Sycophila (Hymenoptera, Eurytomidae). Mol Ecol Resour 10:31–40 CrossRefPubMedGoogle Scholar Liu J, Li Q, Kong L, Yu H, Zheng X (2011) Identifying the true oysters (Bivalvia:Ostreidae) with mitochondrial phylogeny and distance-based DNA barcoding. Mol Ecol Resour 11:820–830 CrossRefPubMedGoogle Scholar Lopez-Osorio F, Pickett KM, Carpenter JM, Ballif BA, Agnarsson I (2017) Phylogenomic analysis of yellowjackets and hornets (Hymenoptera: Vespidae, Vespinae). Mol Phylogenet Evol 107:10–15 CrossRefPubMedGoogle Scholar Mc Donnell RJ, Rugman-Jones P, Backeljau T, Breugelmans K, Jordaens K, Stouthamer R, Paine T, Gormally M (2011) Molecular identification of the exotic slug Arion subfuscus sensu stricto (Gastropoda: Pulmonata) in California, with comments on the source location of introduced populations. Biol Invas 13:61–66 CrossRefGoogle Scholar Meier R, Zhang G, Ali F (2008) The use of mean instead of smallest interspecific distances exaggerates the size of the “barcoding gap” and leads to misidentification. Syst Biol 57:809–813 CrossRefPubMedGoogle Scholar Miller KB, Alarie Y, Wolfe GW, Whiting MF (2005) Association of insect life stages using DNA sequences: the larvae of Philodytes umbrinus (Motschulsky) (Coleoptera: Dytiscidae). Syst Entomol 30:499–509 CrossRefGoogle Scholar Murría C, Zamora-Muñoz C, Bonada N, Ribera C, Prat N (2010) Genetic and morphological approaches to the problematic presence of three Hydropsyche species of the pellucidula group (Trichoptera:Hydropsychidae) in the western most Mediterranean Basin. Aquat Insects 32:85–98 CrossRefGoogle Scholar Nugroho H, Kojima J, Carpenter JM (2012) Checklist of vespid species occurring in Indonesian archipelago. Treubia 38:71–186 Google Scholar Pauls SU, Blahnik RJ, Zhou X, Wardwell CT, Holzenthal RW (2010) DNA barcode data confirm new species and reveal cryptic diversity in Chilean Smicridea (Smicridea) (Trichoptera: Hydropsychidae). J N Am Benthol Soc 29:1058–1074 CrossRefGoogle Scholar Perrard A, Lopez-Osorio F, Carpenter JM (2016) Phylogeny, landmark analysis and the use of wing venation to study the evolution of social wasps (Hymenoptera: Vespidae: Vespinae). Cladistics 32:406–425 CrossRefGoogle Scholar Pickett KM, Carpenter MJ (2010) Simultaneous analysis and the origin of eusociality in the Vespidae (Insecta: Hymenoptera). Arthropod Syst Phylogeny 68:3–33 Google Scholar Saito F, Kojima J (2011) Phylogenetic analysis and biogeography of the nocturnal hornets, Provespa (Insecta: Hymenoptera: Vespidae: Vespinae). Species Divers 16:65–74 Google Scholar Schmid-Egger C, van Achterberg K, Neumeyer R, Morinière J, Schmidt S (2017) Revision of the West Palaearctic Polistes Latreille, with the descriptions of two species—an integrative approach using morphology and DNA barcodes (Hymenoptera, Vespidae). Zookeys 713:53–112 CrossRefGoogle Scholar Silva-Brandão KL, Lyra ML, Freitas AVL (2009) Barcoding Lepidoptera: current situation and perspectives on the usefulness of a contentious technique. Neotropical Entomology 38:441–451 CrossRefPubMedGoogle Scholar Sutou M, Kato T, Ito T (2007) Description of the final larval stage and the pupa of Ctenosciara japonica (Diptera: Sciaridae) and their DNA barcodes. Studia Dipterologica 14:17–22 Google Scholar Tibbetts EA (2007) Dispersal decisions and predispersal behavior in Polistes paper wasp workers. Behav Ecol Sociobiol 61:1877–1883 https://doi.org/10.1007/s00265-007-0427-x CrossRefGoogle Scholar Waugh J (2007) DNA barcoding in animal species: progress, potential and pitfalls. BioEssays 29:188–197 CrossRefPubMedGoogle Scholar Zalat SM (1992) The Vespidae (social wasps) of Egypt. J Egypt Ger Soc Zool 9(D):247–258 Google Scholar Zhang M, Kang L (2005) Genetic divergence among geographical populations of the migratory locust in China. Sci China Ser C Life Sci 48(6):551–564 CrossRefGoogle Scholar Žurovcová MA, Havelka JAN, Starý PETR, Vcchtová PA, Chundelová DA (2010) “DNA barcoding” is of limited value for identifying adelgids (Hemiptera: Adelgidae) but supports traditional morphological taxonomy. Eur J Entomol 107:147–156