Effect of the Method of Preparation on the Composition and Cytotoxic Activity of the Essential Oil of Pituranthos tortuosus
Date
2011
Authors
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Zeitschrift für Naturforschung
Series Info
Z. Naturforsch.;66
Doi
Scientific Journal Rankings
Abstract
The aerial parts of Pituranthos tortuosus (Desf.) Benth and Hook (Apiaceae), growing
wild in Egypt, yielded 0.8%, 0.6%, and 1.5% (v/w) of essential oil when prepared by hydrodistillation
(HD), simultaneous hydrodistillation-solvent (n-pentane) extraction (Lickens-
Nickerson, DE), and conventional volatile solvent extraction (preparation of the “absolute”,
SE), respectively. GC-MS analysis showed that the major components in the HD sample
were β-myrcene (18.81%), sabinene (18.49%), trans-iso-elemicin (12.90%), and terpinen-
4-ol (8.09%); those predominent in the DE sample were terpinen-4-ol (29.65%), sabinene
(7.38%), γ-terpinene (7.27%), and β-myrcene (5.53%); while the prominent ones in the SE
sample were terpinen-4-ol (15.40%), dill apiol (7.90%), and allo-ocimene (4E,6Z) (6.00%).
The oil prepared in each case was tested for its cytotoxic activity on three human cancer cell
lines, i.e. liver cancer cell line (HEPG2), colon cancer cell line (HCT116), and breast cancer
cell line (MCF7). The DE sample showed the most potent activity against the three human
cancer cell lines (with IC50 values of 1.67, 1.34, and 3.38 μg/ml against the liver, colon, and
breast cancer cell lines, respectively). Terpinen-4-ol, sabinene, γ-terpinene, and β-myrcene
were isolated from the DE sample and subjected to a similar evaluation of cytotoxic potency;
signifi cant activity was observed.
Description
MSA Google Scholar
Keywords
University of Pituranthos tortuosus
Citation
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