Comparative Study among the Germination and Propagation of Different Capsicum Annuum Cultivars using Tissue Culture Techniques
| dc.Affiliation | October University for modern sciences and Arts (MSA) | |
| dc.contributor.author | M. Akram, Omar | |
| dc.contributor.author | Gaafar, Mona | |
| dc.contributor.author | Safwat, Gehan | |
| dc.contributor.author | Diab, Ayman A | |
| dc.date.accessioned | 2019-10-21T06:45:54Z | |
| dc.date.available | 2019-10-21T06:45:54Z | |
| dc.date.issued | 2011 | |
| dc.description | MSA Google Scholar | |
| dc.description.abstract | Morphogenetic potential of seed and explants culturing of three capsicum annuum L. ( pepper) genotypes (Gedion, Moaz, and Mohand) were studied to evaluate different plant regeneration protocols and develop a reliable system for plant propagation. An efficient procedure of in vitro plant regeneration through seed and direct shoot bud induction was tested from different explants of capsicum annuum L. Several methods of media preparation with combinations of growth regulators were used, and 2 combinations were found ideal for seed propagation with 7 mg/l 2,4-D or 5 mg/l IBA respectively. Additionally, it was indicated that seed dormancy can effect its propagation also the positioning of the seed or explants onto the media. Propagated seeds demonstrated shoot and root elongation. Regeneration of the explants did not show satisfactory results because most of the explants did not develop into normal shoots but instead developed into calli after 15 days of culture. HPLC Analysis of cultivars demonstrated that fructose suger percentage was higher in the three different types of pepper, followed by sucrose then glucose. Also the HPLC analysis drew out that media supplemented with 7mg/l 2,4-D generally had the highest effect of capsaicinoid sugar content in the following order i.e. cultivar Mohand was showed high significant in sugar content of capsaicinoid followed by Gedion and Moaz. Application of DPPH method illustrated that extracts obtained from Moaz leaves characterized the most proactive antioxidant (vitamin C) properties than Gedion and Mohand. A were noticed. The results of these studies indicated that antioxidant activities of the extract prepared from pepper leaves depended mainly on phenolic compounds. [Omar M. Akram, Mona Gaafar, Gehan Safwat and Ayman Diab. Comparative Study among the Germination and Propagation of Different Capsicum Annuum Cultivars using Tissue Culture Techniques | en_US |
| dc.description.sponsorship | Nature and Science | en_US |
| dc.identifier.citation | Agrawal, S., Chandra, N., and Kothari S.L., 1988. Shoot –tip culture of pepper for micropropagation. Curr. Sci., 57: 1347-1349 Agrawal, S., Chandra N., and Kothari S.L., 1988. Plant regeneration in tissue culture of pepper (capsicum annuum L. cv. Mathania). Plant cell tissue organ culture, 16: 47-55. Andrzej, B., Szczytt, K.N., and Magdalena, J. 2002. Plant regeneration from sweet pepper (capsicum annuum L.) hypocotyls explants. Acta Physiol, Plantarum, 24:257-264. AOAC. 1984. Official Method of Analysis of the Association of Official Analytical Chemists, Sidny Williams. Ed. 14th Ed. Association of Official Analutical Chemists, Inc. Arlington, Virginia, U.S.A Arous, S,. Boussaid, M., and Marrakchi M., 2001. Plant regeneration from zygotic embryo hypocotyls of Tunisian chilli (capsicum annuum L.). J, Applied Hortic., 3:17-22. Arroyo, R., and Revilla, M.A., 1991, In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars. Plant Cell Rep., 10, 414-416. Bray, H.G. and W.V. Thorp. 1954. Analysis of phenolic compounds of interest in metabolism. In: Methods of Biochemical Analysis (Ed.): David Glich, 1: 27-52. Burda S., Oleszek, W., 2001. Antioxidant and antiradical activities of flavonoids. J. Agric. Food Chem. 49, 2774-2779. Christopher, T., and. Rajam M.V, 1994. In vitro clonal propagation of capsicum spp. Plant Cell Tissus Organ Culture, 38:25-29. Christopher, T., and. Rajam M.V, 1996. Effect of genotype explants and medium on Iin vitroI regeneration of red pepper. Plant cell tissue Organ Culture, 46: 245-250. Ezura, H., Nishimiya, S., and Kasumi, M., 1993. Efficient regeneration of plants independent of exogenous growth regulators in bell pepper (Capsicum annuum L.). Plant Cell Rep., 12, 676-680. Gunay, A.L., and Rao P.S., 1978, In vitro plant regeneration from hypocotyl and cotyledon explants of red pepper (Capsicum). Plant Sci. Letts., 11, 365-372. Harini, I. and Sita, G.L., 1993. Direct somatic embryos of chili (capsicum annuum L.). Plant Sci., 89: 107-112. Hussain, S., Jain, A., and Kothari S.L, 1999. Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in (capsicum annum L.). Plant Cell Report, 19: 64-68. Hyde, C. L., and Philips G.C., 1996. Silver nitrate promotes shoot development and plant generation of chili pepper (capsicum annuum L.). via organogenesis. In vitro Cell Dev. Biol. Plants, 32: 72-80. Jung JW, Cho MC, and Cho YS., 2006. Fruit quality of once-over harvest pepper (capsicum annuum. L.) Cultivar ‘Saengryeg No 211’ and ‘Saengryeg No 213’. Kor. J.Hort.Sci. Technol.24(2):205-206. Lope-Hernandez J., Oruna-Concha MJ, Simal-Lozana J, Vazquez-Balnoco ME and Gonzalez-Castro MJ. 1996. Chemical composition of pardon pepper (capsicum annuum. L.) grown in Galicia. Food Chem. 57:557-559. Moore, S.; Spackman, D. H., and Stein. W. H. 1958. Chromatography of amino acid on sulfonated polystyrene resins. Anal. Chem. 30 (7): 1185-1190. Morrison, R,A., Koning, R.E., and Evans, D.A. 1986.Handbook of Plant Cell Culture. Pepper, In: D.A. Evans, W. R. Sharp and P. V. Ammirato (eds.). Macmillan, New York. Vol.4. p. 552-573. Philips, G.C., and Hubstenberger, J.F., 1985. Organogenesis in pepper tissue cultures. Plant Cell Tissues Organ Culture, 4: 261-269. PN-A-04019, 1998. Produkty spoýywcze – Oznaczanie zawartosci witaminy C [Food products – Determination of vitamin C content; in Polish]. Pottino, B. G. ,1981. Methods in plant tissue culture. Maryland : Dept. of Hort., Agric. College. 8-29 pp. Radwan, S. S. 1978. Coupling of two dimention thin layer chromatography with gas chromatography for the quantitative analysis of lipids classes and their constituent fayyy acids. J. Chromatog. Sci. 16: 538-542. Ramirez-Malagon, R., and Ochoa-Alejo, N., 1996, An improved and reliable chilli pepper (Capsicum annuum L.) plant regeneration method. Plant Cell Rep., 16, 226-231. Roe J., 1967. Ascorbic acid. The Vitamins 7, 27-35. Steel, R. G. D., and Torrie, J. H. 1980. Principles and procedures of statistics. A biometrical approach. 2nd ed. McGraw-Hill Kogakusha, Ltd., pp. 633. Szasz, A., Nervo, G., and Fari, M., 1995, Screening for in vitro shoot forming capacity of seedling explants in bell pepper (Capsicum annuum L.) genotypes and efficient plant regeneration using thidiazuron. Plant Cell Rep., 14, 666-669. Thomas, W., and Dutcher, R. A. 1924. Picric acid method for carbohydrate J. Amer. Chem. Sci. 46: 162-166 | en_US |
| dc.identifier.issn | 1545-0740 | |
| dc.identifier.uri | http://central-library.msa.edu.eg:8009/xmlui/handle/123456789/458 | |
| dc.language.iso | en | en_US |
| dc.publisher | Nature and Science | en_US |
| dc.relation.ispartofseries | Nature and Science;9 (7) :183-189 | |
| dc.subject | University for Capsicum Annuum L | en_US |
| dc.subject | micropropagation | en_US |
| dc.title | Comparative Study among the Germination and Propagation of Different Capsicum Annuum Cultivars using Tissue Culture Techniques | en_US |
| dc.type | Article | en_US |