Modulating Effect of Green Tea and Vitamin C in the Management of “Alzheimer's disease: Oxidative Stress, Cell-Based Anti-Inflammatory and Genotoxic Profiles

Abstract

Alzheimer's disease is a progressive brain disorder. Green tea (GT) and vitamin C are known for their antioxidant and anti-inflammatory properties. This study aims to investigate the potential oxidative stress, cell-based anti-inflammatory and genotoxic Profiles for synergistic effect of green tea and vitamin c against to Alzheimer's disease. In vitro assessments included their several antioxidant parameters and ability to inhibit inflammatory markers such as nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and matrix metallopeptidase 1(MPP1) in lipopolysaccharide-activated macrophages. In vivo approach evaluated genotoxicity and their potential neurotherapy against Alzheimer's disease using a rat model. Behavioral, biochemical, and histological evaluations were conducted. Green tea methanolic extract (GTME) and vitamin C showed enhanced antioxidant and anti-inflammatory modulator activities. The neurotoxicity rat’s model exhibited cognitive decline and brain injury in contrast to the control group. While treated rats with GTME and vitamin C exhibited improved cognitive function, behavior and reduced AChE levels the combination exerted a significantly more potent effect. Histological analysis confirmed improved brain tissue structure in rats receiving the combined treatment. Crucially, neither compound exhibited genotoxic properties. The combination of GTME and vitamin C demonstrated a promising therapeutic potential for neurodegenerative diseases due to their synergistic effects.