Sensitivity Enhancement for Direct Injection Capillary Electrophoresis to Determine Morphine in Human Serum via In-capillary Derivatization
| dc.Affiliation | October University for modern sciences and Arts (MSA) | |
| dc.contributor.author | Emara S. | |
| dc.contributor.author | Zarad W. | |
| dc.contributor.author | Kamal M. | |
| dc.contributor.author | Ali A. | |
| dc.contributor.author | Aboulella Y. | |
| dc.contributor.other | Pharmaceutical Chemistry Department | |
| dc.contributor.other | Faculty of Pharmacy | |
| dc.contributor.other | Misr International University | |
| dc.contributor.other | Km 28 Ismailia Road | |
| dc.contributor.other | Cairo | |
| dc.contributor.other | 44971 | |
| dc.contributor.other | Egypt; Pharmaceutical Analytical Chemistry Department | |
| dc.contributor.other | Faculty of Pharmacy | |
| dc.contributor.other | Modern Sciences and Arts University | |
| dc.contributor.other | 26 July Mehwar Road intersection with Wahat Road 6 | |
| dc.contributor.other | October City | |
| dc.contributor.other | 12573 | |
| dc.contributor.other | Egypt; Laboratory for Single Cell Mass Spectrometry | |
| dc.contributor.other | RIKEN Quantitative Biology Center | |
| dc.contributor.other | 6-2-3 | |
| dc.contributor.other | Furuedai | |
| dc.contributor.other | Suita | |
| dc.contributor.other | Osaka | |
| dc.contributor.other | 565-0874 | |
| dc.contributor.other | Japan | |
| dc.date.accessioned | 2020-01-09T20:40:41Z | |
| dc.date.available | 2020-01-09T20:40:41Z | |
| dc.date.issued | 2019 | |
| dc.description | Scopus | |
| dc.description.abstract | Rapid and simple micellar electrokinetic chromatography (MEKC) with in-capillary derivatization and fluorescence detection has been developed to determine morphine in human serum. The sample was introduced into a background electrolyte (BGE) containing potassium ferricyanide, whereas morphine was oxidized into highly fluorescent product, pseudomorphine. Different parameters for derivatization and subsequent separation were systematically investigated for the analysis of morphine in serum. Efficient performance of the developed MEKC system was carried out in a single run using BGE made up of 70 mM sodium tetraborate decahydrate (pH 10.5), 0.30 mM potassium ferrricyanide, 80 mM sodium dodecyl sulfate, and applied voltage of 9 kV. The combination of MEKC with in-capillary derivatization of morphine was successfully achieved with a high degree of sensitivity. The validation of the method showed good linearity between areas of morphine and the corresponding concentrations over the range of 5-5000 ng/mL. Excellent accuracy and precision were obtained at all concentration levels. The mean recoveries of morphine were ranging from 83.86 to 94.45%. The validated MEKC method successfully permitted determination of morphine in clinical samples after a single oral dose of controlled release morphine sulfate tablets. � 2018 The Author(s). | en_US |
| dc.description.uri | https://www.scimagojr.com/journalsearch.php?q=24023&tip=sid&clean=0 | |
| dc.identifier.doi | https://doi.org/10.1093/chromsci/bmy092 | |
| dc.identifier.doi | PubMed ID 30272145 | |
| dc.identifier.issn | 219665 | |
| dc.identifier.other | https://doi.org/10.1093/chromsci/bmy092 | |
| dc.identifier.other | PubMed ID 30272145 | |
| dc.identifier.uri | https://t.ly/GgJ79 | |
| dc.language.iso | English | en_US |
| dc.publisher | Oxford University Press | en_US |
| dc.relation.ispartofseries | Journal of Chromatographic Science | |
| dc.relation.ispartofseries | 57 | |
| dc.subject | morphine | en_US |
| dc.subject | blood | en_US |
| dc.subject | capillary electrophoresis | en_US |
| dc.subject | human | en_US |
| dc.subject | limit of detection | en_US |
| dc.subject | micellar electrokinetic chromatography | en_US |
| dc.subject | pH | en_US |
| dc.subject | procedures | en_US |
| dc.subject | reproducibility | en_US |
| dc.subject | spectrofluorometry | en_US |
| dc.subject | statistical model | en_US |
| dc.subject | Chromatography, Micellar Electrokinetic Capillary | en_US |
| dc.subject | Electrophoresis, Capillary | en_US |
| dc.subject | Humans | en_US |
| dc.subject | Hydrogen-Ion Concentration | en_US |
| dc.subject | Limit of Detection | en_US |
| dc.subject | Linear Models | en_US |
| dc.subject | Morphine | en_US |
| dc.subject | Reproducibility of Results | en_US |
| dc.subject | Spectrometry, Fluorescence | en_US |
| dc.title | Sensitivity Enhancement for Direct Injection Capillary Electrophoresis to Determine Morphine in Human Serum via In-capillary Derivatization | en_US |
| dc.type | Article | en_US |
| dcterms.isReferencedBy | Smith, H.S., The metabolism of opioid agents and the clinical impact of their active metabolites (2011) The Clinical Journal of Pain, 27 (9), pp. 824-838; Payne, R., Mathias, S.D., Pasta, D.J., Wanke, L.A., Williams, R., Mahmoud, R., Quality of life and cancer pain: Satisfaction and side effects with transdermal fentanyl versus oral morphine (1998) Journal of Clinical Oncology, 16 (4), pp. 1588-1593; Stout, P.R., Bynum, N.D., Mitchell, J.M., Baylor, M.R., Ropero-Miller, J.D., A comparison of the validity of gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry analysis of urine samples for morphine, codeine, 6-acetylmorphine, and benzoylecgonine (2009) Journal of Analytical Toxicology, 33 (8), pp. 398-408; Goldberger, B.A., Chronister, C.W., Merves, M.L., Quantitation of opioids in blood and urine using gas chromatography-mass spectrometry (GC-MS) (2010) Methods in Molecular Biology, 603, pp. 399-410; Hofmann, U., Seefried, S., Schweizer, E., Ebner, T., Mikus, G., Eichelbaum, M., Highly sensitive gas chromatographic-tandem mass spectrometric method for the determination of morphine and codeine in serum and urine in the femtomolar range (1999) Journal of Chromatography B, 727 (1-2), pp. 81-88; Weinmann, W., Renz, M., Vogt, S., Pollak, S., Automated solid-phase extraction and two-step derivatisation for simultaneous analysis of basic illicit drugs in serum by GC/MS (2000) International Journal of Legal Medicine, 113 (4), pp. 229-235; Dahn, T., Gunn, J., Kriger, S., Terrell, A.R., Quantitation of morphine, codeine, hydrocodone, hydromorphone, oxycodone, oxymorphone, and 6-monoacetylmorphine (6-MAM) in urine, blood, serum, or plasma using liquid chromatography with tandem mass spectrometry detection (2010) Methods in Molecular Biology, 603, pp. 411-422; Eckart, K., R�hrich, J., Breitmeier, D., Ferner, M., Laufenberg-Feldmann, R., Urban, R., Development of a new multi-analyte assay for the simultaneous detection of opioids in serum and other body fluids using liquid chromatography-tandem mass spectrometry (2015) Journal of Chromatography B, 1001, pp. 1-8; Langman, L.J., Korman, E., Stauble, M.E., Boswell, M.V., Baumgartner, R.N., Jortani, S.A., Therapeutic monitoring of opioids: A sensitive lc-ms/ ms method for quantitation of several opioids including hydrocodone and its metabolites (2013) Therapeutic Drug Monitoring, 35 (3), pp. 352-359; Sartori, D., Lewis, T., Breaud, A., Clarke, W., The development of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous quantification of morphine, morphine-3-?-glucuronide, morphine-6-?-glucuronide, hydromorphone, and normorphine in serum (2015) Clinical Biochemistry, 48 (18), pp. 1283-1290; Szkutnik-Fiedler, D., Grze?kowiak, E., Gaca, M., Borowicz, M., Hplcuv determination of morphine in human plasma and its application to the clinical study (2011) Acta Poloniae Pharmaceutica, 68 (4), pp. 473-479; Clavijo, C.F., Hoffman, K.L., Thomas, J.J., Carvalho, B., Chu, L.F., Drover, D.R., A sensitive assay for the quantification of morphine and its active metabolites in human plasma and dried blood spots using high-performance liquid chromatography-tandem mass spectrometry (2011) Analytical and Bioanalytical Chemistry, 400 (3), pp. 715-728; Emara, S., Development of highly sensitive and specific hplc assay for plasma morphine using direct injection technique and post-column derivatization (1998) Biomedical Chromatography, 12 (1), pp. 15-20; Kotb, H.I., El-Kabsh, M.Y., Emara, S.E., Fouad, E.A., Pharmacokinetics of controlled release morphine (MST) in patients with liver cirrhosis (1997) British Journal of Anaesthesia, 79 (6), pp. 804-806; Kotb, H.I., El-Kady, S.A., Emara, S.E., Fouad, E.A., El-Kabsh, M.Y., Pharmacokinetics of controlled release morphine (MST) in patients with liver carcinoma (2005) British Journal of Anaesthesia, 94 (1), pp. 95-99; Chapman, D.J., Joel, S.P., Aherne, G.W., Evaluation of a differential radioimmunoassay technique for the determination of morphine and morphine-6-glucuronide in human plasma (1994) Journal of Pharmaceutical and Biomedical Analysis, 12 (3), pp. 353-360; Schumacher, S., Seitz, H., A novel immunoassay for quantitative drug abuse screening in serum (2016) Journal of Immunological Methods, 436, pp. 34-40; Rodr�guez, J., Contento, A.M., Casta�eda, G., Mu�oz, L., Berciano, M.A., Determination of morphine, codeine, and paclitaxel in human serum and plasma by micellar electrokinetic chromatography (2012) Journal of Separation Science, 35 (17), pp. 2297-2306; Alnajjar, A., Butcher, J.A., McCord, B., Determination of multiple drugs of abuse in human urine using capillary electrophoresis with fluorescence detection (2004) Electrophoresis, 25 (10-11), pp. 1592-1600; Costa, J.L., Morrone, A.R., Resende, R.R., Chasin, A.A., Tavares, M.F., Development of a method for the analysis of drugs of abuse in vitreous humor by capillary electrophoresis with diode array detection (CE-DAD) (2014) Journal of Chromatography B, pp. 84-91. , 945-946; Isbell, T.A., Strickland, E.C., Hitchcock, J., McIntire, G., Colyer, C.L., Capillary electrophoresis-mass spectrometry determination of morphine and its isobaric glucuronide metabolites (2015) Journal of Chromatography B, 980, pp. 65-71; Mi, J.Q., Zhang, X.X., Chang, W.B., Determination of morphine by capillary zone electrophoresis immunoassay combined with laser-induced fluorescence detection (2004) Journal of Immunoassay and Immunochemistry, 25 (1), pp. 57-70; Taylor, R.B., Low, A.S., Reid, R.G., Determination of opiates in urine by capillary electrophoresis (1996) Journal of Chromatography B, 675 (2), pp. 213-223; Wey, A.B., Thormann, W., Head-column field-amplified sample stacking in presence of siphoning: Application to capillary electrophoresis-electrospray ionization mass spectrometry of opioids in urine (2001) Journal of Chromatography. A, 924 (1-2), pp. 507-518; Zhang, X.X., Li, J., Gao, J., Sun, L., Chang, W.B., Determination of morphine by capillary electrophoresis immunoassay in thermally reversible hydrogel-modified buffer and laser-induced fluorescence detection (2000) Journal of Chromatography. A, 895 (1-2), pp. 1-7; Emara, S., Darwish, I., Youssef, D., Masujima, T., On the perspectives of capillary electrophoresis modes for the determination of morphine in human plasma without sample pretreatment (2004) Biomedical Chromatography, 18 (1), pp. 21-27; Bardelmeijer, H.A., Waterval, J.C., Lingeman, H., Vant Hof, R., Bult, A., Underberg, W.J., Pre-on-and post-column derivatization in capillary electrophoresis (1997) Electrophoresis, 18 (12-13), pp. 2214-2227; Taga, A., Sugimura, M., Honda, S., Derivatization of amino acids in a moving zone of o-phthalaldehyde in the middle of a capillary for amino acid analysis by capillary electrophoresis (1998) Journal of Chromatography. A, 802 (1), pp. 243-248; Taga, A., Honda, S., Derivatization at capillary inlet in high-performance capillary electrophoresis Its reliability in quantification (1996) Journal of Chromatography. A, 742 (1-2), pp. 243-250; Taga, A., Nishino, A., Honda, S., Characteristic features of the throughout-capillary technique of in-capillary derivatization in capillary electrophoresis (1998) Journal of Chromatography. A, 822 (2), pp. 271-279; Wintersteige, R., Barary, M.H., El-Yazbi, F.A., Sabry, S.M., Wahbi, A.-A.M., Fluorimetric determination of morphine in codeine (1994) Spectroscopy Letters, 27 (10), pp. 1447-1460; Nelson, P.E., Nolan, S.L., Bedford, K., High-performance liquid chromatography detection of morphine by fluorescence after post-column derivatisation (1982) Journal of Chromatography, 234 (2), pp. 407-414; Nelson, P.E., High-performance liquid chromatography detection of morphine by fluorescence after post-column derivatisation: II. The effect of micelle formation (1984) Journal of Chromatography. A, 298, pp. 59-65; Aguilar, M., Huang, X., Zare, R.N., Determination of metal ion complexes in electroplating solutions using capillary zone electrophoresis with UV detection (1989) Journal of Chromatography. A, 480, pp. 427-431 | |
| dcterms.source | Scopus |