Ethosomes and lipid-coated chitosan nanocarriers for skin delivery of a chlorophyll derivative: A potential treatment of squamous cell carcinoma by photodynamic therapy

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dc.AffiliationOctober University for modern sciences and Arts (MSA)
dc.contributor.authorNasr S.
dc.contributor.authorRady M.
dc.contributor.authorGomaa I.
dc.contributor.authorSyrovet T.
dc.contributor.authorSimmet T.
dc.contributor.authorFayad W.
dc.contributor.authorAbdel-Kader M.
dc.contributor.otherInstitute of Pharmacology of Natural Products & Clinical Pharmacology
dc.contributor.otherUlm University
dc.contributor.otherUlm
dc.contributor.otherD-89081
dc.contributor.otherGermany; Department of Chemistry
dc.contributor.otherSchool of Sciences and Engineering
dc.contributor.otherAmerican University in Cairo (AUC)
dc.contributor.otherEgypt; Pharmaceutical Technology Department
dc.contributor.otherFaculty of Pharmacy and Biotechnology
dc.contributor.otherGerman University in Cairo (GUC). Main Entrance of Al-Tagamoa Al-Khames New Cairo City
dc.contributor.otherEgypt; Faculty of Pharmacy
dc.contributor.otherOctober University for Modern Sciences and Arts (MSA)
dc.contributor.otherEgypt; Drug Bioassay-Cell Culture Laboratory
dc.contributor.otherPharmacognosy Department
dc.contributor.otherNational Research Centre
dc.contributor.otherDokki
dc.contributor.otherGiza
dc.contributor.other12622
dc.contributor.otherEgypt; National Institute of Laser Enhanced Sciences (NILES)
dc.contributor.otherCairo University (CU)
dc.contributor.otherGiza
dc.contributor.otherEgypt
dc.date.accessioned2020-01-09T20:40:33Z
dc.date.available2020-01-09T20:40:33Z
dc.date.issued2019
dc.descriptionScopus
dc.description.abstractPhotodynamic therapy (PDT) is a localized treatment strategy used for skin cancers such as squamous cell carcinoma (SCC), the second most common form of skin cancer. PDT combines a photosensitizer, laser source and tissue oxygen. In this study, the selected photosensitizer, ferrous chlorophyllin (Fe-CHL) was loaded in ethosomes and lipid coated chitosan (PC/CHI) nanocarriers to enhance skin delivery of Fe-CHL for potential PDT of squamous carcinoma. The nanocarrier formulations were characterized and studied for their skin retention and penetration depth of Fe-CHL across mouse skin ex vivo using high performance liquid chromatography and confocal microscopy. Confocal microscope images of mouse skin showed deeper penetration of ethosomes down to the dermis when compared to PC/CHI that was confined to the epidermis, although they showed no significant difference in skin retention. Immunohistochemistry (IHC) staining with HE, ki67 and TUNEL show maintained skin structure and no cytotoxic effects of the nanocarrier gel formulations before laser exposure to mouse skin. The nanocarriers were also studied for their PDT effect against human SCC monolayer and three-dimensional (3-D) spheroids. When compared to ethosomes, PC/CHI showed higher cytotoxicity in MTT assay and live confocal microscopy showed cell disintegration after laser exposure. For 3-D spheroids, PC/CHI also showed higher cytotoxicity using acid phosphatase assay and a decrease in spheroid size was observed using light microscopy. In conclusion, both types of nanocarriers can be used for their potential treatment of SCC using PDT depending on the tumour localization in the skin. � 2019 Elsevier B.V.en_US
dc.description.urihttps://www.scimagojr.com/journalsearch.php?q=22454&tip=sid&clean=0
dc.identifier.doihttps://doi.org/10.1016/j.ijpharm.2019.118528
dc.identifier.doiPubMed ID 31323373
dc.identifier.issn3785173
dc.identifier.otherhttps://doi.org/10.1016/j.ijpharm.2019.118528
dc.identifier.otherPubMed ID 31323373
dc.identifier.urihttps://t.ly/JX385
dc.language.isoEnglishen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofseriesInternational Journal of Pharmaceutics
dc.relation.ispartofseries568
dc.subjectChlorophyll derivativesen_US
dc.subjectNanocarriersen_US
dc.subjectPhotodynamic therapyen_US
dc.subjectSkin canceren_US
dc.subjectSkin deliveryen_US
dc.subjectacid phosphataseen_US
dc.subjectchitosan nanoparticleen_US
dc.subjectchlorophyllen_US
dc.subjectKi 67 antigenen_US
dc.subjectanimal tissueen_US
dc.subjectArticleen_US
dc.subjectconfocal microscopyen_US
dc.subjectcontrolled studyen_US
dc.subjectcytotoxicity testen_US
dc.subjectdrug delivery systemen_US
dc.subjectdrug formulationen_US
dc.subjectex vivo studyen_US
dc.subjecthigh performance liquid chromatographyen_US
dc.subjectimmunohistochemistryen_US
dc.subjectmicroscopyen_US
dc.subjectmouseen_US
dc.subjectMTT assayen_US
dc.subjectnonhumanen_US
dc.subjectphotodynamic therapyen_US
dc.subjectpriority journalen_US
dc.subjectskin structureen_US
dc.subjectsquamous cell skin carcinomaen_US
dc.subjecttumor localizationen_US
dc.subjectTUNEL assayen_US
dc.subjectzeta potentialen_US
dc.titleEthosomes and lipid-coated chitosan nanocarriers for skin delivery of a chlorophyll derivative: A potential treatment of squamous cell carcinoma by photodynamic therapyen_US
dc.typeArticleen_US
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